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Ligand 4-nitrophenyl octanoate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (36 results)

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Show Substrate (36 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.1

p-nitrophenyl octanoate + H2O = p-nitrophenol + octanoate

285594

-

3.1.1.1

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

670779, 653767, 669032, 667247, 653765, 721892, 680960, 692120, 715403, 668716, 719148

-

3.1.1.1

4-nitrophenyl octanoate + H2O = 4 nitrophenol + octanoate

725749

-

3.1.1.1

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

171001, 170988, 171021, 729255, 171029, 649395, 652627, 723816, 713856, 171022, 652181, 667780, 714366, 727876, 721413, 722994, 725745, 725749, 724065, 726707, 729856, 749622, 678016, 679887, 669032, 713766, 713786, 724578, 724971, 728547, 726693, 743730, 749674

-

3.1.1.1

p-nitrophenyl caprylate + H2O = p-nitrophenol + caprylate

677751

-

3.1.1.1

p-nitrophenyl octanoate = p-nitrophenol + decanoate

677663

-

3.1.1.1

p-nitrophenyl octanoate = p-nitrophenol + octanoate

677663

-

3.1.1.1

6 entries

3.1.1.108

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

753524

-

3.1.1.13

p-nitrophenyl caprylate + H2O = p-nitrophenol + caprylic acid

133824

-

3.1.1.13

p-nitrophenyl octanoate + H2O = p-nitrophenol + octanoic acid

664194, 664868

-

3.1.1.2

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

750166, 651605, 692889, 693586, 729872

-

3.1.1.2

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

652628

-

3.1.1.23

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylic acid

651780

-

3.1.1.23

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

651084

-

3.1.1.3

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

666830, 691356, 682608, 653738, 665288, 679881, 679885, 667017, 651025, 692415, 650306, 681358, 650654, 666825, 714583, 713783, 713778, 714856, 715206, 714501

-

3.1.1.3

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

682643, 692254, 722957, 741707, 651141, 650688, 653102, 694108, 716181, 727566

-

3.1.1.13

2 entries

3.1.1.2

2 entries

3.1.1.23

2 entries

3.1.1.3

2 entries

3.1.1.4

p-nitrophenyl octanoate + H2O = p-nitrophenol + octanoic acid

666811

-

3.1.1.41

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

651823

-

3.1.1.5

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

729689

-

3.1.1.60

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

750754

-

3.1.1.72

p-nitrophenyl caprylate + H2O = p-nitrophenol + caprylate

697655

-

3.1.1.73

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

715967

-

3.1.1.73

p-nitrophenyl caprylate + H2O = p-nitrophenol + caprylate

707264

-

3.1.1.74

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

730129, 730094

-

3.1.1.74

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

208331, 729653, 208318, 729000

-

3.1.1.73

2 entries

3.1.1.74

2 entries

3.1.1.75

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

730415, 664977

-

3.1.1.76

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

715799, 665995, 679540

-

3.1.1.76

4-nitrophenyl octanoate + H2O = ?

729063, 729031

-

3.1.1.76

4-nitrophenyl octanoate + H2O = octanoate + 4-nitrophenol

716724, 713824

-

3.1.1.79

4-nitrophenol caprylate + H2O = 4-nitrophenol + caprylate

750177

-

3.1.1.79

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

750496, 750161

-

3.1.1.79

p-nitrophenyl caprylate + H2O = ?

690779

-

3.1.1.88

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

732458

-

3.1.1.88

4-nitrophenyl octanoate + H2O = 4-nitrophenol + octanoate

751477, 751480

-

3.4.19.1

4-nitrophenyl caprylate + H2O = 4-nitrophenol + caprylate

754201

-

3.4.19.1

p-nitrophenyl caprylate + H2O = nitrophenol + caprylate

669473

-

3.1.1.76

3 entries

3.1.1.79

3 entries

3.1.1.88

2 entries

3.4.19.1

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (51 results)

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Show Turnover Number (51 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.1

0.24

-

-

285594

3.1.1.1

0.0313

-

pH 7.0, 70°C, mutant enzyme D524A

727876

3.1.1.1

0.032

-

pH 7.0, 70°C, mutant enzyme D524N

727876

3.1.1.1

0.125

-

pH 7.0, 37°C

667247

3.1.1.1

0.5

-

in 2.5 mM Bicine buffer, pH 8.3, at 30°C

729255

3.1.1.1

1.11

-

recombinant mutant NaM1H2, pH 8.5, 30°C

749674

3.1.1.1

1.7

-

pH 7.4, 37°C, wild-type enzyme

719148

3.1.1.1

6.36

-

pH 8.0, 80°C

729856

3.1.1.1

7.8

-

pH 7.4, 37°C, mutant enzyme R210A

719148

3.1.1.1

8.67

-

pH 7.0, 70°C, wild-type enzyme

727876

3.1.1.1

13

-

60°C, pH 7.5

677751

3.1.1.1

21.1

-

pH 9.0, 80°C

725749

3.1.1.1

24

-

wild-type, pH 8.0, 25°C

667780

3.1.1.1

25.2

-

-

679887

3.1.1.1

25.2

-

pH 7.5, 70°C

679887

3.1.1.1

51

-

pH 8.0, 50°C

649395

3.1.1.1

79.3

-

pH 7.0, 70°C

724578

3.1.1.1

360

-

pH 7.0, 30°C

669032

3.1.1.1

360

-

pH 7.0, 30°C

669032

3.1.1.1

692

-

37°C

171022

3.1.1.1

770

-

70°C, pH 7.1

721413

3.1.1.1

831

-

80°C, pH 8.0, wild-type enzyme

723816

3.1.1.1

881

-

60°C

171022

3.1.1.1

1230

-

45°C

171022

3.1.1.1

1250

-

-

171021

3.1.1.1

1376

-

mutant M211V, pH 7.1, 70°C

652181

3.1.1.1

1380

-

mutant M211V, pH 7.1, 70°C

652181

3.1.1.1

1491

-

pH 8.0, 60°C

725745

3.1.1.1

1600

-

pH 7.1, 70°C

726707

3.1.1.1

1645

-

mutant M211S/R215L, pH 7.1, 70°C

652181

3.1.1.1

1650

-

mutant M211S/R215L, pH 7.1, 70°C

652181

3.1.1.1

1740

-

mutant M211T, pH 7.1, 70°C

652181

3.1.1.1

1855

-

mutant M211S, pH 7.1, 70°C

652181

3.1.1.1

1860

-

mutant M211S, pH 7.1, 70°C

652181

3.1.1.1

1960

-

wild-type enzyme, pH 7.1, 70°C

652181

3.1.1.1

3080

-

mutant R215L, pH 7.1, 70°C

652181

3.1.1.1

35 entries

3.1.1.108

1.24

-

at pH 7.5 and 55°C

753524

3.1.1.2

410

-

recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C

692889

3.1.1.3

24.05

-

wild-type, pH 8.0, 45°C

741707

3.1.1.3

42.34

-

mutant T18K/T22I/E230I, pH 8.0, 55°C

741707

3.1.1.3

43.71

-

mutant T18K/T22I/S56C/N63C/V189C/E230I/D238C, pH 8.0, 60°C

741707

3.1.1.3

55.12

-

mutant enzyme S154L/S293L, at pH 6.0 and 25°C

715206

3.1.1.3

4 entries

3.1.1.74

187

-

pH 7.5, 30°C, recombinant enzyme

729653

3.1.1.88

149.7

-

pH 6.5, 55°C

732458

3.4.19.1

6.6

-

80°C, wild-type enzyme

669473

3.4.19.1

8.1

-

80°C, mutant enzyme R526E

669473

3.4.19.1

9.4

-

80°C, mutant enzyme R526K

669473

3.4.19.1

9.6

-

80°C, mutant enzyme R526A

669473

3.4.19.1

20.8

-

80°C, mutant enzyme R526I

669473

3.4.19.1

28.5

-

80°C, mutant enzyme R526L

669473

3.4.19.1

30.9

-

80°C, mutant enzyme R526V

669473

3.4.19.1

7 entries

K_M Value (63 results)

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Show KM Value (63 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.1.1.1

0.0047

-

60°C, pH 7.5

677751

3.1.1.1

0.0117

-

pH 7.0, 70°C, mutant enzyme D524N

727876

3.1.1.1

0.012

-

-

171021

3.1.1.1

0.014

-

pH 7.0, 37°C

667247

3.1.1.1

0.0165

-

37°C

171022

3.1.1.1

0.017

-

37°C

670779

3.1.1.1

0.022

-

mutant M211S/R215L, pH 7.1, 70°C

652181

3.1.1.1

0.0232

-

pH 7.0, 70°C, wild-type enzyme

727876

3.1.1.1

0.025

-

mutant R215L, pH 7.1, 70°C

652181

3.1.1.1

0.0254

-

45°C

171022

3.1.1.1

0.0266

-

pH 7.0, 70°C, mutant enzyme D524A

727876

3.1.1.1

0.028

-

pH 7.4, 37°C, wild-type enzyme

719148

3.1.1.1

0.028

-

wild-type enzyme, pH 7.1, 70°C

652181

3.1.1.1

0.029

-

pH 8.0, 60°C

725745

3.1.1.1

0.033

-

in 2.5 mM Bicine buffer, pH 8.3, at 30°C

729255

3.1.1.1

0.0335

-

60°C

171022

3.1.1.1

0.037

-

mutant M211S, pH 7.1, 70°C

652181

3.1.1.1

0.04

-

wild-type, pH 8.0, 25°C

667780

3.1.1.1

0.043

-

pH 7.4, 37°C, mutant enzyme R210A

719148

3.1.1.1

0.051

-

pH 7.1, 70°C

726707

3.1.1.1

0.06

-

mutant M211V, pH 7.1, 70°C

652181

3.1.1.1

0.0606

-

pH 8.0, 25°C

678016

3.1.1.1

0.068

-

pH 7.0, 30°C

669032

3.1.1.1

0.068

-

pH 7.0, 30°C

669032

3.1.1.1

0.072

-

pH 7.5, 30°C

652627

3.1.1.1

0.1

-

mutant M211T, pH 7.1, 70°C

652181

3.1.1.1

0.119

-

70°C, pH 7.1

721413

3.1.1.1

0.15

-

-

171001

3.1.1.1

0.152

-

pH 9.0, 80°C

725749

3.1.1.1

0.153

-

pH 9.0, 80°C

725749

3.1.1.1

0.19

-

recombinant mutant NaM1H2, pH 8.5, 30°C

749674

3.1.1.1

0.2

-

pH 8.0, 50°C

649395

3.1.1.1

0.289

-

80°C, pH 8.0, wild-type enzyme

723816

3.1.1.1

0.63

-

-

679887

3.1.1.1

2.64

-

pH 8.0, 80°C

729856

3.1.1.1

14.5

-

pH 7.0, 70°C

724578

3.1.1.1

25.2

-

pH 7.5, 70°C

679887

3.1.1.1

37 entries

3.1.1.108

0.005

-

at pH 7.5 and 55°C

753524

3.1.1.13

0.144

-

mutant G505D/S506D, pH 7.4, 37°C

664194

3.1.1.13

0.316

-

mutant S506D, pH 7.4, 37°C

664194

3.1.1.13

1.639

-

mutant S506N, pH 7.4, 37°C

664194

3.1.1.13

1.782

-

mutant S506K, pH 7.4, 37°C

664194

3.1.1.2

0.028

-

recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C

692889

3.1.1.2

0.23

-

EstB, pH 7.5, 30°C

652628

3.1.1.3

0.27

-

mutant enzyme S154L/S293L, at pH 6.0 and 25°C

715206

3.1.1.3

0.31

-

in Tris-HCl buffer (50 mM, pH 8.2), at 37°C

692415

3.1.1.3

1.17

-

wild-type, pH 8.0, 45°C

741707

3.1.1.3

1.53

-

mutant T18K/T22I/S56C/N63C/V189C/E230I/D238C, pH 8.0, 60°C

741707

3.1.1.3

2.01

-

mutant T18K/T22I/E230I, pH 8.0, 55°C

741707

3.1.1.74

0.207

-

pH 7.5, 30°C, recombinant enzyme

729653

3.1.1.74

0.59

-

cutinase II

208318

3.1.1.74

0.88

-

cutinase I

208318

3.1.1.74

1.7

-

-

208331

3.1.1.74

7.24

-

pH 8.0, 25°C, recombinant enzyme

730094

3.1.1.13

4 entries

3.1.1.2

2 entries

3.1.1.3

5 entries

3.1.1.74

5 entries

3.1.1.76

0.122

-

pH 9.0, 37°C

729063

3.1.1.88

0.0332

-

pH 6.5, 55°C

732458

3.4.19.1

35.7

-

80°C, mutant enzyme R526V

669473

3.4.19.1

38.2

-

80°C, mutant enzyme R526K

669473

3.4.19.1

38.2

-

80°C, mutant enzyme R526L

669473

3.4.19.1

40.1

-

80°C, mutant enzyme R526I

669473

3.4.19.1

41.6

-

80°C, mutant enzyme R526A

669473

3.4.19.1

43.3

-

80°C, wild-type enzyme

669473

3.4.19.1

114.6

-

80°C, mutant enzyme R526E

669473

3.4.19.1

7 entries

References & Links

Literature References (119)

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Show Reference (119 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

133824

8753777

Catalytic properties of the purified rat hepatic cytosolic cholesteryl ester hydrolase

1996

Natarajan, R.; Ghosh, S.; McLean Grogan, W.

Biochem. Biophys. Res. Commun.

225

413-419

170988

6468391

Plant biochemistry of xenobiotics. Purification and properties of a wheat esterase hydrolyzing the plasticizer chemical, bis(2-ethylhexyl)phthalate

1984

Krell, H.W.; Sandermann, H.

Eur. J. Biochem.

143

57-62

171001

738990

Purification and enzymatic properties of rat serum carboxylesterase

1978

Hashinotsume, M.; Higashino, K.; Hada, T.; Yamamura, Y.

J. Biochem.

84

1325-1333

171021

7576531

Purification and partial characterization of a novel thermophilic carboxylesterase with high mesophilic specific activity

1995

Wood, A.N.P.; Fernandez-Lafuente, R.; Cowan, D.A.

Enzyme Microb. Technol.

17

816-825

171022

8181479

Purification and characterization of a thermostable carboxylesterase from the thermoacidophilic eubacterium Bacillus acidocaldarius

1994

Manco, G.; di Gennaro, S.; de Rosa, M.; Rossi, M.

Eur. J. Biochem.

221

965-972

171029

-

Purification and some properties of carboxylesterase from Arthrobacter globiformis stereoselective hydrolysis of ethyl chrysanthemate

1993

Nishizawa, M.; Gomi, H.; Kishimoto, F.

Biosci. Biotechnol. Biochem.

57

594-598

208318

239740

Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi

1975

Purdy, R.E.; Kolattukudy, P.E.

Biochemistry

14

2832-2840

208331

3130804

Purification and characterization of cutinase from a fluorescent Pseudomonas putida bacterial strain isolated from phyllosphere

1988

Sebastian, J.; Kolattukudy, P.E.

Arch. Biochem. Biophys.

263

77-85

285594

7041828

Multifunctionality of liver alcohol dehydrogenase: kinetic and mechanistic studies of esterase reaction

1982

Tsai, C.S.

Arch. Biochem. Biophys.

213

635-642

649395

10968629

Purification and characterization of a thermostable esterase from the moderate thermophile Bacillus circulans

2000

Kademi, A.; Ait-Abdelkader, N.; Fakhreddine, L.; Baratti, J.

Appl. Microbiol. Biotechnol.

54

173-179

650306

10825531

Overproduction in Escherichia coli, purification and characterization of a family I.3 lipase from Pseudomonas sp. MIS38

2000

Amada, K.; Haruki, M.; Imanaka, T.; Morikawa, M.; Kanaya, S.

Biochim. Biophys. Acta

1478

201-210

650654

14550014

Production, purification, characterization, and applications of lipases

2001

Sharma, R.; Chisti, Y.; Banerjee, U.C.

Biotechnol. Adv.

19

627-662

650688

-

Properties of recombinant Rhizomucor miehei lipase with amino acid substitutions of Phe94 in the substrate binding domain

2001

Oh, S.W.; Gaskin, D.J.H.; Kwon, D.Y.; Vulfson, E.N.

Biotechnol. Lett.

23

563-568

651025

-

Biochemical characterization of a recombinant thermoalkalophilic lipase and assessment of its substrate enantioselectivity

2002

Sunna, A.; Hunter, L.; Hutton, C.A.; Bergquist, P.L.

Enzyme Microb. Technol.

31

472-476

651084

11029590

A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase

2000

Eggert, T.; Pencreac'h, G.; Douchet, I.; Verger, R.; Jaeger, K.E.

Eur. J. Biochem.

267

6459-6469

651141

12444965

Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1

2002

Snellman, E.A.; Sullivan, E.R.; Colwell, R.R.

Eur. J. Biochem.

269

5771-5779

651605

10792515

Characterization of an arylesterase from Lactobacillus helveticus CNRZ32

2000

Fenster, K.M.; Parkin, K.L.; Steele, J.L.

J. Appl. Microbiol.

88

572-583

651780

11226879

Monoacylglycerol lipase from moderately thermophilic Bacillus sp. strain H-257: molecular cloning, sequencing, and expression in Escherichia coli of the gene

2001

Kitaura, S.; Suzuki, K.; Imamura, S.

J. Biochem.

129

397-402

651823

-

Isolation, analysis, and expression of lipase with cephalosporin-C deacetylation activity from Staphylococcus sp

2001

Lee, H.W.; Ko, J.Y.; Kim, W.J.; Byun, S.M.

J. Biochem. Mol. Biol.

34

274-277

652181

11447219

Residues at the active site of the esterase 2 from Alicyclobacillus acidocaldarius involved in substrate specificity and catalytic activity at high temperature

2001

Manco, G.; Mandrich, L.; Rossi, M.

J. Biol. Chem.

276

37482-37490

652627

12741535

Intracellular esterase from Lactobacillus casei LILA: nucleotide sequencing, purification, and characterization

2003

Fenster, K.M.; Parkin, K.L.; Steele, J.L.

J. Dairy Sci.

86

1118-1129

652628

12939078

Nucleotide sequencing, purification, and biochemical properties of an arylesterase from Lactobacillus casei LILA

2003

Fenster, K.M.; Parkin, K.L.; Steele, J.L.

J. Dairy Sci.

86

2547-2557

653102

11337984

Purification and characterization of an extracellular lipase from Penicillium candidum

2001

Ruiz, B.; Farres, A.; Langley, E.; Masso, F.; Sanchez, S.

Lipids

36

283-289

653738

11483000

Optimization of a thermostable lipase from Bacillus stearothermophilus P1: overexpression, purification, and characterization

2001

Sinchaikul, S.; Sookkheo, B.; Phutrakul, S.; Pan, F.M.; Chen, S.T.

Protein Expr. Purif.

22

388-398

653765

12729729

Construction and characterization of a recombinant esterase with high activity and enantioselectivity to (S)-ketoprofen ethyl ester

2003

Choi, G.S.; Kim, J.Y.; Kim, J.H.; Ryu, Y.W.; Kim, G.J.

Protein Expr. Purif.

29

85-93

653767

12880765

Cloning, heterologous expression, renaturation, and characterization of a cold-adapted esterase with unique primary structure from a psychrotroph Pseudomonas sp. strain B11-1

2003

Suzuki, T.; Nakayama, T.; Choo, D.W.; Hirano, Y.; Kurihara, T.; Nishino, T.; Esaki, N.

Protein Expr. Purif.

30

171-178

664194

15866484

Acidic residues emulate a phosphorylation switch to enhance the activity of rat hepatic neutral cytosolic cholesterol esterase

2005

Milad-Kodsi, E.; Langston, T.B.; Gergis, M.R.; Grogan, W.M.

Biochim. Biophys. Acta

1734

62-73

664868

16463077

Purification, characterization, and molecular cloning of organic-solvent-tolerant cholesterol esterase from cyclohexane-tolerant Burkholderia cepacia strain ST-200

2006

Takeda, Y.; Aono, R.; Doukyu, N.

Extremophiles

10

269-177

664977

12855176

Identification and characterisation of the catalytic triad of the alkaliphilic thermotolerant PHA depolymerase PhaZ7 of Paucimonas lemoignei

2003

Braaz, R.; Handrick, R.; Jendrossek, D.

FEMS Microbiol. Lett.

224

107-112

665288

16448188

Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase

2006

Chang, S.W.; Lee, G.C.; Shaw, J.F.

J. Agric. Food Chem.

54

815-822

665995

15995648

Molecular characterization of extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase genes from Pseudomonas alcaligenes strains

2005

Kim, D.Y.; Kim, H.C.; Kim, S.Y.; Rhee, Y.H.

J. Microbiol.

43

285-294

666811

15135393

A novel phospholipase A2/esterase from hyperthermophilic archaeon Aeropyrum pernix K1

2004

Wang, B.; Lu, D.; Gao, R.; Yang, Z.; Cao, S.; Feng, Y.

Protein Expr. Purif.

35

199-205

666825

15596365

High-level heterologous expression and properties of a novel lipase from Ralstonia sp. M1

2005

Quyen, D.T.; Le, T.T.G.; Nguyen, T.T.; Oh, T.K.; Lee, J.K.

Protein Expr. Purif.

39

97-106

666830

15802219

Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3

2005

Kumar, S.; Kikon, K.; Upadhyay, A.; Kanwar, S.S.; Gupta, R.

Protein Expr. Purif.

41

38-44

667017

15942926

An extracellular lipase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ALIP1 gene and characterization of the purified recombinant enzyme

2005

Böer, E.; Mock, H.P.; Bode, R.; Gellissen, G.; Kunze, G.

Yeast

22

523-535

667247

15184114

Characterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96

2004

Choi, Y.J.; Miguez, C.B.; Lee, B.H.

Appl. Environ. Microbiol.

70

3213-3221

667780

14726205

Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly->Pro substitution near the active site on its catalytic activity and stability

2004

Kulakova, L.; Galkin, A.; Nakayama, T.; Nishino, T.; Esaki, N.

Biochim. Biophys. Acta

1696

59-65

668716

15033540

Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus

2004

Ewis, H.E.; Abdelal, A.T.; Lu, C.D.

Gene

329

187-195

669032

16448191

Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112

2006

Wu, P.C.; Liu, Y.H.; Wang, Z.Y.; Zhang, X.Y.; Li, H.; Liang, W.Q.; Luo, N.; Hu, J.M.; Lu, J.Q.; Luan, T.G.; Cao, L.X.

J. Agric. Food Chem.

54

836-842

669473

16670095

Discrimination of esterase and peptidase activities of acylaminoacyl peptidase from hyperthermophilic Aeropyrum pernix K1 by a single mutation

2006

Wang, Q.; Yang, G.; Liu, Y.; Feng, Y.

J. Biol. Chem.

281

18618-18625

670779

16269247

A novel esterase from Bacillus subtilis (RRL 1789): purification and characterization of the enzyme

2006

Kaiser, P.; Raina, C.; Parshad, R.; Johri, S.; Verma, V.; Andrabi, K.I.; Qazi, G.N.

Protein Expr. Purif.

45

262-268

677663

16672512

Isolation and biochemical characterization of two novel metagenome-derived esterases

2006

Elend, C.; Schmeisser, C.; Leggewie, C.; Babiak, P.; Carballeira, J.D.; Steele, H.L.; Reymond, J.; Jaeger, K.; Streit, W.R.

Appl. Environ. Microbiol.

72

3637-3645

677751

17106678

Characterization of a thermostable carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima

2007

Kakugawa, S.; Fushinobu, S.; Wakagi, T.; Shoun, H.

Appl. Microbiol. Biotechnol.

74

585-591

678016

3128284

Purification and characterization of a heat-stable esterase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius.

1988

Sobek, H.; Görisch, H.

Biochem. J.

250

453-458

679540

-

Characterization of an extracellular poly(3-hydroxyoctanoate) depolymerase from the marine isolate, Pseudomonas luteola M13-4

2006

Rhee, Y.H.; Kim, Y.H.; Shin, K.

Enzyme Microb. Technol.

38

529-535

679881

17328767

Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment

2007

Hardeman, F.; Sjoeling, S.

FEMS Microbiol. Ecol.

59

524-534

679885

12480114

Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase

2002

Ruiz, C.; Blanco, A.; Pastor, F.I.; Diaz, P.

FEMS Microbiol. Lett.

217

263-267

679887

15212797

A novel thermostable esterase from the thermoacidophilic archaeon Sulfolobus tokodaii strain 7.

2004

Suzuki, Y.; Miyamoto, K.; Ohta, H.

FEMS Microbiol. Lett.

236

97-102

680960

16621096

Molecular cloning of carboxylesterase gene and biochemical characterization of encoded protein from Bacillus subtilis (RRL BB1)

2006

Maqbool, Q.U.; Johri, S.; Rasool, S.; Riyaz-ul-Hassan, S.; Verma, V.; Nargotra, A.; Koul, S.; Qazi, G.N.

J. Biotechnol.

125

1-10

681358

18051356

Molecular cloning and functional expression of esf gene encoding enantioselective lipase from Serratia marcescens ES-2 for kinetic resolution of optically active (S)-flurbiprofen

2007

Lee, K.W.; Bae, H.A.; Lee, Y.H.

J. Microbiol. Biotechnol.

17

74-80

682608

-

Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt

2007

Soliman, N.A.; Knoll, M.; Abdel-Fattah, Y.R.; Schmid, R.D.; Lange, S.

Process Biochem.

42

1090-1100

682643

16290008

Purification and properties of a novel extracellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate

2006

Kanwar, S.S.; Ghazi, I.A.; Chimni, S.S.; Joshi, G.K.; Rao, G.V.; Kaushal, R.K.; Gupta, R.; Punj, V.

Protein Expr. Purif.

46

421-428

690779

19116143

Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase

2009

Nam, K.H.; Kim, M.Y.; Kim, S.J.; Priyadarshi, A.; Lee, W.H.; Hwang, K.Y.

Biochem. Biophys. Res. Commun.

379

553-556

691356

18809324

Membrane-bound synthetic lipase specifically cultured under solid-state fermentation and submerged fermentation by Rhizopus chinensis: A comparative investigation

2008

Sun, S.Y.; Xu, Y.

Bioresour. Technol.

100

1336-1342

692120

-

Esterase activity of bovine serum albumin up to 160 °C: A new benchmark for biocatalysis

2008

Cordova, J.; Ryan, J.D.; Boonyaratanakornkit, B.B.; Clark, D.S.

Enzyme Microb. Technol.

42

278-283

692254

18437283

The cold-active Lip1 lipase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 is a member of a new bacterial lipolytic enzyme family

2008

de Pascale, D.; Cusano, A.M.; Autore, F.; Parrilli, E.; di Prisco, G.; Marino, G.; Tutino, M.L.

Extremophiles

12

311-323

692415

19055033

Partial characterization of a lipase from gypsy moth (Lymantria dispar L.) larval midgut

2008

Mrdakovic, M.; Lazarevic, J.; Peric-Mataruga, V.; Ilijin, L.; Vlahovic, M.

Folia Biol. (Krakow)

56

103-110

692889

18931117

A novel thermostable arylesterase from the archaeon Sulfolobus solfataricus P1: purification, characterization, and expression

2008

Park, Y.J.; Yoon, S.J.; Lee, H.B.

J. Bacteriol.

190

8086-8095

693586

18337701

Cloning and characterization of thermostable esterase from Archaeoglobus fulgidus

2008

Kim, S.B.; Lee, W.; Ryu, Y.W.

J. Microbiol.

46

100-107

694108

19107605

Gene cloning, expression, and characterization of the Geobacillus thermoleovorans CCR11 thermoalkaliphilic lipase

2008

Quintana-Castro, R.; Diaz, P.; Valerio-Alfaro, G.; Garcia, H.S.; Oliart-Ros, R.

Mol. Biotechnol.

42

75-83

697655

22578869

Purification and properties of an acetylxylan esterase from Thermobifida fusca

2008

Yang, C.; Liu, W.

Enzyme Microb. Technol.

42

181-186

707264

20127235

Thermostable feruloyl esterase for the bioproduction of ferulic acid from triticale bran

2010

Abokitse, K.; Wu, M.; Bergeron, H.; Grosse, S.; Lau, P.C.

Appl. Microbiol. Biotechnol.

87

195-203

713766

-

Purification and characterization of a novel extracellular carboxylesterase from the moderately halophilic bacterium Thalassobacillus sp. strain DF-E4

2011

Lv, X.; Guo, L.; Song, L.; Fu, Q.; Zhao, K.; Li, A.; Luo, X.; Lu, W.

Ann. Microbiol.

61

281-290

713778

20574645

Molecular and enzymatic characterization of a subfamily I.4 lipase from an edible oil-degrader Bacillus sp. HH-01

2011

Kamijo, T.; Saito, A.; Ema, S.; Yoh, I.; Hayashi, H.; Nagata, R.; Nagata, Y.; Ando, A.

Antonie van Leeuwenhoek

99

179-187

713783

20177822

Isolation and characterization of a novel thermophilic-organic solvent stable lipase from Acinetobacter baylyi

2010

Uttatree, S.; Winayanuwattikun, P.; Charoenpanich, J.

Appl. Biochem. Biotechnol.

162

1362-1376

713786

20237863

Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01

2010

Liu, X.; Xu, J.H.; Pan, J.; Zhao, J.

Appl. Biochem. Biotechnol.

162

1574-1584

713824

20400568

Production of chiral (R)-3-hydroxyoctanoic acid monomers, catalyzed by Pseudomonas fluorescens GK13 poly(3-hydroxyoctanoic acid) depolymerase

2010

Gangoiti, J.; Santos, M.; Llama, M.J.; Serra, J.L.

Appl. Environ. Microbiol.

76

3554-3560

713856

21908637

A novel metagenome-derived carboxylesterase that hydrolyzes beta-lactam antibiotics

2011

Jeon, J.H.; Kim, S.J.; Lee, H.S.; Cha, S.S.; Lee, J.H.; Yoon, S.H.; Koo, B.S.; Lee, C.M.; Choi, S.H.; Lee, S.H.; Kang, S.G.; Lee, J.H.

Appl. Environ. Microbiol.

77

7830-7836

714366

20850500

Insights into the fatty acid chain length specificity of the carboxylesterase PA3859 from Pseudomonas aeruginosa: A combined structural, biochemical and computational study

2010

Pesaresi, A.; Lamba, D.

Biochimie

92

1787-1792

714501

21145735

Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis

2011

Dheeman, D.S.; Henehan, G.T.; Frias, J.M.

Biores. Technol.

102

3373-3379

714583

21805187

Cloning, expression and characterization of a new lipase from Yarrowia lipolytica

2011

Zhao, H.; Zheng, L.; Wang, X.; Liu, Y.; Xu, L.; Yan, Y.

Biotechnol. Lett.

33

2445-2452

714856

25919623

Enzymatic properties and expression patterns of five extracellular lipases of Fusarium graminearum in vitro

2010

Nguyen, L.; Dao, T.; Zivkovic, T.; Fehrholz, M.; Schaefer, W.; Salomon, S.

Enzyme Microb. Technol.

46

479-486

715206

21747717

A novel cold-active lipase from Candida albicans: cloning, expression and characterization of the recombinant enzyme

2011

Lan, D.M.; Yang, N.; Wang, W.K.; Shen, Y.F.; Yang, B.; Wang, Y.H.

Int. J. Mol. Sci.

12

3950-3965

715403

20587647

A novel thermostable carboxylesterase from Geobacillus thermodenitrificans: evidence for a new carboxylesterase family

2010

Charbonneau, D.M.; Meddeb-Mouelhi, F.; Beauregard, M.

J. Biochem.

148

299-308

715799

21104001

Characterization of a novel immobilized biocatalyst obtained by matrix-assisted refolding of recombinant polyhydroxyoctanoate depolymerase from Pseudomonas putida KT2442 isolated from inclusion bodies

2011

Arroyo, M.; Garcia-Hidalgo, J.; Villalon, M.; de Eugenio, L.; Hormigo, D.; Acebal, C.; Garcia, J.L.; Prieto, M.A.; de la Mata, I.

J. Ind. Microbiol. Biotechnol.

38

1203-1209

715967

21757926

Molecular cloning, overexpression and characterization of a novel feruloyl esterase from a soil metagenomic library

2011

Sang, S.L.; Li, G.; Hu, X.P.; Liu, Y.H.

J. Mol. Microbiol. Biotechnol.

20

196-203

716181

21678056

Characterization of a thermostable lipase showing loss of secondary structure at ambient temperature

2012

Sharma, P.K.; Singh, K.; Singh, R.; Capalash, N.; Ali, A.; Mohammad, O.; Kaur, J.

Mol. Biol. Rep.

39

2795-2804

716724

-

Purification and characterization of an extracellular medium-chain length polyhydroxyalkanoate depolymerase from Thermus thermophilus HB8

2011

Papaneophytou, C.; Velali, E.; Pantazaki, A.

Polym. Degrad. Stab.

96

670-678

719148

20386955

Biochemical characterization and mutational improvement of a thermophilic esterase from Sulfolobus solfataricus P2

2010

Shang, Y.S.; Zhang, X.E.; Wang, X.D.; Guo, Y.C.; Zhang, Z.P.; Zhou, Y.F.

Biotechnol. Lett.

32

1151-1157

721413

10620337

Cloning, overexpression, and properties of a new thermophilic and thermostable esterase with sequence similarity to hormone-sensitive lipase subfamily from the archaeon Archaeoglobus fulgidus

2000

Manco, G.; Giosue, E.; DAuria, S.; Herman, P.; Carrea, G.; Rossi, M.

Arch. Biochem. Biophys.

373

182-192

721892

-

Thermophilic esterase from the archaeon Archaeoglobus fulgidus physically immobilized on hydrophobic macroporous resin: A novel biocatalyst for polyester synthesis

2011

Li, G.; Li, Q.

Biotechnol. Bioprocess Eng.

16

1201-1207

722957

19447113

Structure of the alkalohyperthermophilic Archaeoglobus fulgidus lipase contains a unique C-terminal domain essential for long-chain substrate binding

2009

Chen, C.K.; Lee, G.C.; Ko, T.P.; Guo, R.T.; Huang, L.M.; Liu, H.J.; Ho, Y.F.; Shaw, J.F.; Wang, A.H.

J. Mol. Biol.

390

672-685

722994

-

Ring-opening polymerization of epsilon-caprolactone catalyzed by a novel thermophilic esterase from the archaeon Archaeoglobus fulgidus

2009

Ma, J.; Li, Q.; Song, B.; Liu, D.; Zheng, B.; Zhang, Z.; Feng, Y.

J. Mol. Catal. B

56

151-157

723816

23099882

Alteration of substrate specificities of thermophilic alpha/beta hydrolases through domain swapping and domain interface optimization

2012

Zhou, X.; Wang, H.; Zhang, Y.; Gao, L.; Feng, Y.

Acta Biochim. Biophys. Sin.

44

965-973

724065

21847512

Exceptional thermal stability and organic solvent tolerance of an esterase expressed from a thermophilic host

2012

Mei, Y.; Peng, N.; Zhao, S.; Hu, Y.; Wang, H.; Liang, Y.; She, Q.

Appl. Microbiol. Biotechnol.

93

1965-1974

724578

15564667

Thermostable esterase from a thermoacidophilic archaeon: purification and characterization for enzymatic resolution of a chiral compound

2004

Kim, S.; Lee, S.B.

Biosci. Biotechnol. Biochem.

68

2289-2298

724971

22748144

Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii

2012

Angkawidjaja, C.; Koga, Y.; Takano, K.; Kanaya, S.

FEBS J.

279

3071-3084

725745

-

Cloning, expression, purification, and characterization of a thermostable esterase from the archaeon Sulfolobus solfataricus P1

2013

Nam, J.-K.; Park, Y.-J.; Lee, H.-B.

J. Mol. Catal. B

94

95-103

725749

-

Characterization of a novel thermophilic pyrethroid-hydrolyzing carboxylesterase from Sulfolobus tokodaii into a new family

2013

Weia, T.; Fenga, S.; Shenb, Y.; Hea, P.; Maa, G.; Yua, X.; Zhang, F.; Mao, D.

J. Mol. Catal. B

97

225-232

726693

21643808

Enhanced activity and enantioselectivity of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1 by acetone treatment

2011

Cong, F.; Xing, K.; Gao, R.; Cao, S.; Zhang, G.

Appl. Biochem. Biotechnol.

165

795-801

726707

12147492

Extremely stable and versatile carboxylesterase from a hyperthermophilic archaeon

2002

Hotta, Y.; Ezaki, S.; Atomi, H.; Imanaka, T.

Appl. Environ. Microbiol.

68

3925-3931

727566

24789461

Characterization of an organic solvent-tolerant lipase from Haloarcula sp. G41 and its application for biodiesel production

2014

Li, X.; Yu, H.Y.

Folia Microbiol. (Praha)

59

455-463

727876

21084296

Structure and catalysis of acylaminoacyl peptidase: closed and open subunits of a dimer oligopeptidase

2011

Harmat, V.; Domokos, K.; Menyhard, D.K.; Pallo, A.; Szeltner, Z.; Szamosi, I.; Beke-Somfai, T.; Naray-Szabo, G., Polgar, L.

J. Biol. Chem.

286

1987-1998

728547

19759821

Solution behavior and activity of a halophilic esterase under high salt concentration

2009

Rao, L.; Zhao, X.; Pan, F.; Li, Y.; Xue, Y.; Ma, Y.; Lu, J.R.

PLoS One

4

e6980

729000

23604968

Production optimization and characterization of recombinant cutinases from Thermobifida fusca sp. NRRL B-8184

2013

Hegde, K.; Veeranki, V.D.

Appl. Biochem. Biotechnol.

170

654-675

729031

22865072

Characterization of a novel subgroup of extracellular medium-chain-length polyhydroxyalkanoate depolymerases from actinobacteria

2012

Gangoiti, J.; Santos, M.; Prieto, M.A.; de la Mata, I.; Serra, J.L.; Llama, M.J.

Appl. Environ. Microbiol.

78

7229-7237

729063

22695803

Polyester hydrolytic and synthetic activity catalyzed by the medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces venezuelae SO1

2013

Santos, M.; Gangoiti, J.; Keul, H.; Moeller, M.; Serra, J.L.; Llama, M.J.

Appl. Microbiol. Biotechnol.

97

211-222

729255

24832101

Structural characterization and function determination of a nonspecific carboxylate esterase from the amidohydrolase superfamily with a promiscuous ability to hydrolyze methylphosphonate esters

2014

Xiang, D.F.; Kumaran, D.; Swaminathan, S.; Raushel, F.M.

Biochemistry

53

3476-3485

729653

22579385

Thiol-functionalization of acrylic ester monomers catalyzed by immobilized Humicola insolens cutinase

2012

Kazenwadel, C.; Eiben, S.; Maurer, S.; Beuttler, H.; Wetzl, D.; Hauer, B.; Koschorreck, K.

Enzyme Microb. Technol.

51

9-15

729689

22622648

High level expression and characterization of a thermostable lysophospholipase from Thermococcus kodakarensis KOD1

2012

Cui, Z.; Wang, Y.; Pham, B.P.; Ping, F.; Pan, H.; Cheong, G.W.; Zhang, S.; Jia, B.

Extremophiles

16

619-625

729856

25250909

Biochemical characterization of a carboxylesterase from the archaeon Pyrobaculum sp. 1860 and a rational explanation of its substrate specificity and thermostability

2014

Shao, H.; Xu, L.; Yan, Y.

Int. J. Mol. Sci.

15

16885-16910

729872

24856385

Characterization of a versatile arylesterase from Lactobacillus plantarum active on wine esters

2014

Esteban-Torres, M.; Barcenilla, J.M.; Mancheno, J.M.; de las Rivas, B.; Munoz, R.

J. Agric. Food Chem.

62

5118-5125

730094

24910973

High level expression of Glomerella cingulata cutinase in dense cultures of Pichia pastoris grown under fed-batch conditions

2014

Seman, W.M.; Bakar, S.A.; Bukhari, N.A.; Gaspar, S.M.; Othman, R.; Nathan, S.; Mahadi, N.M.; Jahim, J.; Murad, A.M.; Bakar, F.D.

J. Biotechnol.

184

219-228

730129

23863287

Novel cutinase from Pseudomonas cepacia NRRL B 2320: purification, characterization and identification of cutinase encoding genes

2013

Dutta, K.; Krishnamoorthy, H.; Venkata Dasu, V.

J. Gen. Appl. Microbiol.

59

171-184

730415

24007310

Biochemical analysis and structure determination of Paucimonas lemoignei poly(3-hydroxybutyrate) (PHB) depolymerase PhaZ7 muteins reveal the PHB binding site and details of substrate-enzyme interactions

2013

Jendrossek, D.; Hermawan, S.; Subedi, B.; Papageorgiou, A.C.

Mol. Microbiol.

90

649-664

732458

22409882

Identification and characterization of a novel thermostable pyrethroid-hydrolyzing enzyme isolated through metagenomic approach

2012

Fan, X.; Liu, X.; Huang, R.; Liu, Y.

Microb. Cell Fact.

11

33

741707

29101200

Enhancing the thermostability of Rhizomucor miehei lipase with a limited screening library by rational-design point mutations and disulfide bonds

2018

Li, G.; Fang, X.; Su, F.; Chen, Y.; Xu, L.; Yan, Y.

Appl. Environ. Microbiol.

84

e02129-17

743730

24859676

Thermophilic esterase from Thermomyces lanuginosus molecular cloning, functional expression and biochemical characterization

2014

Li, X.J.; Zheng, R.C.; Wu, Z.M.; Ding, X.; Zheng, Y.G.

Protein Expr. Purif.

101

1-7

749622

27364331

Functional characterization of a marine Bacillus esterase and its utilization in the stereo-selective production of D-methyl lactate

2016

Huang, J.; Zhang, Y.; Hu, Y.

Appl. Biochem. Biotechnol.

180

1467-1481

749674

29453256

Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family

2018

Adesioye, F.A.; Makhalanyane, T.P.; Vikram, S.; Sewell, B.T.; Schubert, W.D.; Cowan, D.A.

Appl. Environ. Microbiol.

84

e02695-17

750161

28044227

A novel, versatile family IV carboxylesterase exhibits high stability and activity in a broad pH spectrum

2017

Dukunde, A.; Schneider, D.; Lu, M.; Brady, S.; Daniel, R.

Biotechnol. Lett.

39

577-587

750166

26443058

Characterization of cross-linked immobilized arylesterase from Gluconobacter oxydans 621H with activity toward cephalosporin C and 7-aminocephalosporanic acid

2016

Inmaculada, N.; Francisco, G.

Biotechnol. Prog.

32

36-42

750177

27222124

Identification of amino acids related to catalytic function of Sulfolobus solfataricus P1 carboxylesterase by site-directed mutagenesis and molecular modeling

2016

Choi, Y.H.; Lee, Y.N.; Park, Y.J.; Yoon, S.J.; Lee, H.B.

BMB Rep.

49

349-354

750496

28710671

Functional characterization of hormone sensitive-like lipase from Bacillus halodurans synthesis and recovery of pNP-laurate with high yields

2017

Dua, A.; Gupta, R.

Extremophiles

21

871-889

750754

-

Biodegradation of seven phthalate esters by Bacillus mojavensis B1811

2018

Zhang, J.; Zhang, C.; Zhu, Y.; Li, J.; Li, X.

Int. Biodeter. Biodegrad.

132

200-207

751477

28893251

Identification and immobilization of a novel cold-adapted esterase, and its potential for bioremediation of pyrethroid-contaminated vegetables

2017

Fan, X.; Liang, W.; Li, Y.; Li, H.; Liu, X.

Microb. Cell Fact.

16

149

751480

28490329

Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostability

2017

Liu, X.; Liang, M.; Liu, Y.; Fan, X.

Microb. Cell Fact.

16

81

753524

31053933

Identification of a novel esterase from the thermophilic bacterium Geobacillus thermodenitrificans NG80-2

2019

Curci, N.; Strazzulli, A.; De Lise, F.; Iacono, R.; Maurelli, L.; Dal Piaz, F.; Cobucci-Ponzano, B.; Moracci, M.

Extremophiles

23

407-419

754201

29235404

Understanding the interactions of different substrates with wild-type and mutant acylaminoacyl peptidase using molecular dynamics simulations

2018

Zhu, J.; Wang, Y.; Li, X.; Han, W.; Zhao, L.

J. Biomol. Struct. Dyn.

36

4285-4302

Links to other databases for 4-nitrophenyl octanoate

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ChEBI

PubChem

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