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Ligand isomaltotriose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.70

isomaltotriose + H2O = ?

732004

-

7.5.2.2

ATP + H2O + isomaltotriose/out = ADP + phosphate + isomaltotriose/in

289127

-

In Vivo Product in Enzyme-catalyzed Reactions (7 results)

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Show Natural Product (7 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.11

dextran + H2O = isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides

0

-

3.2.1.11

dextran T10 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T2000 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T40 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T500 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T70 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

6 entries

7.5.2.2

ATP + H2O + isomaltotriose/out = ADP + phosphate + isomaltotriose/in

289127

-

Substrate in Enzyme-catalyzed Reactions (17 results)

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Show Substrate (17 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.64

beta-D-glucose-1-phosphate + isomaltotriose = ?

674950

-

2.4.1.7

sucrose + isomaltotriose = ?

679550

-

3.2.1.10

isomaltotriose + H2O = 3 alpha-D-glucose

326251, 326246, 326254, 326261, 690556

-

3.2.1.10

isomaltriose + H2O = ?

654304, 729747

-

3.2.1.10

2 entries

3.2.1.11

isomaltotriose + H2O = ?

135730

-

3.2.1.20

isomaltotriose + H2O = ?

692850

-

3.2.1.20

isomaltotriose + H2O = alpha-D-glucose

393269, 393280, 393346, 393292, 393347, 393298, 393353, 393270

-

3.2.1.20

2 entries

3.2.1.205

isomaltotriose + 2 H2O = beta-D-glucose + 2 D-glucose

742891

-

3.2.1.3

isomaltotriose + H2O = ?

136199, 136231

-

3.2.1.3

isomaltotriose + H2O = ? + beta-D-glucose

663981

-

3.2.1.3

isomaltotriose + H2O = beta-D-glucose

654806

-

3.2.1.70

isomaltotriose + H2O = ?

732004

-

3.2.1.70

isomaltotriose + H2O = beta-D-glucose

208777, 208778

-

3.2.1.70

isomaltotriose + H2O = D-glucose

699532, 664233

-

3.2.1.3

3 entries

3.2.1.70

3 entries

3.2.1.94

isomaltotriose + H2O = alpha-isomaltose + D-glucose

137017, 137018, 655809, 655808

-

5.4.99.11

isomaltotriose = ?

746984

-

7.5.2.2

ATP + H2O + isomaltotriose/out = ADP + phosphate + isomaltotriose/in

289127

-

Product in Enzyme-catalyzed Reactions (35 results)

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Show Product (35 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.5

sucrose + isomaltose = D-fructose + isomaltotriose

0

-

3.2.1.11

dextran + H2O = d-glucose + isomaltose + isomaltotriose + ?

0

-

3.2.1.11

dextran + H2O = D-glucose + isomaltotriose + isomaltose + isomaltooligosaccharide

0

-

3.2.1.11

dextran + H2O = isomaltose + isomaltotriose + D-glucose + isomaltooligosaccharides

0

-

3.2.1.11

dextran + H2O = isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides

0

-

3.2.1.11

dextran + H2O = isomaltotriose

0

-

3.2.1.11

dextran + H2O = isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T10 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T10 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

dextran T100 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

dextran T20 + H2O = isomaltose + isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T2000 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T2000 + H2O = isomaltose + isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T2000 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

dextran T2000 + H2O = isomaltotriose + isomaltotetraose + isomaltopentaose

0

-

3.2.1.11

dextran T250 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

dextran T3 + H2O = isomaltose + isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T40 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T40 + H2O = isomaltose + isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T40 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

dextran T5 + H2O = isomaltose + isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T500 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T500 + H2O = isomaltose + isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T500 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

dextran T60-90 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

dextran T70 + H2O = isomaltose + isomaltotriose

0

-

3.2.1.11

dextran T70 + H2O = isomaltose + isomaltotriose + D-glucose

0

-

3.2.1.11

dextran T70 + H2O = isomaltose + isomaltotriose + isomaltooligosaccharides

0

-

3.2.1.11

glucan + H2O = D-glucose + isomaltose + isomaltotriose + ?

0

-

3.2.1.11

isomaltohexaose + H2O = D-glucose + isomaltose + isomaltotriose + isomaltotetraose + isomaltopentaose

0

-

3.2.1.11

29 entries

3.2.1.115

2(3)-O-alpha-D-glucosyl-isomaltotriose + H2O = isomaltotriose + alpha-D-glucose

0

-

3.2.1.20

isomaltose = isomaltotriose + alpha-D-glucose

393345

-

3.2.1.95

dextran + H2O = isomaltotriose

208775, 0

-

3.2.1.95

isomaltodextrin + H2O = isomaltotriose

208775

-

3.2.1.95

2 entries

7.5.2.2

ATP + H2O + isomaltotriose/out = ADP + phosphate + isomaltotriose/in

289127

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (12 results)

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Show Turnover Number (12 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.10

12

-

-

690556

3.2.1.10

12.5

-

-

690556

3.2.1.10

15

-

pH 7.0, 30°C

729747

3.2.1.10

18

-

pH 7.0, 30°C

729747

3.2.1.10

21

-

pH 7.0, 30°C

729747

3.2.1.10

5 entries

3.2.1.205

1.07

-

pH 6.0, 35°C

742891

3.2.1.3

0.64

-

recombinant glucoamylase

654806

3.2.1.70

12.2

-

pH 6.0, 37°C, mutant enzyme W238N

664233

3.2.1.70

33.1

-

pH 6.0, 37°C, mutant enzyme W238A

664233

3.2.1.70

77.5

-

pH 6.0, 37°C, mutant enzyme W238P

664233

3.2.1.70

475

-

purified recombinant enzyme, pH 6.0, 37°C

732004

3.2.1.70

541

-

pH 6.0, 37°C, wild-type enzyme

664233

3.2.1.70

5 entries

K_M Value (19 results)

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Show KM Value (19 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.10

1.7

-

-

326261

3.2.1.10

3

-

-

326251

3.2.1.10

4.4

-

-

326246

3.2.1.10

5.5

-

-

690556

3.2.1.10

11

-

-

326254

3.2.1.10

16.7

-

-

690556

3.2.1.10

72

-

pH 7.0, 30°C

729747

3.2.1.10

83

-

pH 7.0, 30°C

729747

3.2.1.10

128

-

pH 7.0, 30°C

729747

3.2.1.11

25

-

-

135730

3.2.1.11

31

-

-

135730

3.2.1.10

9 entries

3.2.1.11

2 entries

3.2.1.20

6.7

-

-

393346

3.2.1.205

4.36

-

pH 6.0, 35°C

742891

3.2.1.3

18

-

recombinant glucoamylase

654806

3.2.1.70

3.17

-

pH 6.0, 37°C, wild-type enzyme

664233

3.2.1.70

4.96

-

pH 6.0, 37°C, mutant enzyme W238N

664233

3.2.1.70

10.2

-

pH 6.0, 37°C, mutant enzyme W238P

664233

3.2.1.70

11.4

-

pH 6.0, 37°C, mutant enzyme W238A

664233

3.2.1.70

14.1

-

purified recombinant enzyme, pH 6.0, 37°C

732004

3.2.1.70

5 entries

References & Links

Literature References (37)

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Show Reference (37 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

135730

4755424

Studies on mold dextranases

1973

Hiraoka, N.; Tsuji, H.; Fukumoto, J.; Yamamoto, T.; Tsuru, D.

Int. J. Pept. Protein Res.

5

161-169

136199

6434506

Fungal glucoamylases

1983

Manjunath, P.; Shenoy, B.C.; Raghavendra Roa, M.R.

J. Appl. Biochem.

5

235-260

136231

8679632

Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering

1996

Fierobe, H.P.

Biochemistry

35

8696-8704

137017

-

Quantitative study of the anomeric forms of isomaltose and glucose produced by isomalto-dextranase and glucodextranase

1987

Takayanagi, T.; Okada, G.; Chiba, S.

Agric. Biol. Chem.

51

2337-2341

137018

-

Purification and some properties of the isomaltodextranase of Actinomadura strain R10 and comparison with that of Arthrobacter globiformis T6

1981

Sawai, T.; Ohara, S.; Ichimi, Y.; Okaji, S.; Hisada, K.; Fukaya, N.

Carbohydr. Res.

89

289-299

208775

4210084

Studies on dextranase. II. New exo-dextranase from Brevibacterium fuscum var. Dextranlyticum

1974

Sugiura, M.; Ito, A.; Yamaguchi, T.

Biochim. Biophys. Acta

350

61-70

208777

2380687

Nucleotide sequence of the dextran glucosidase (dexB) gene of Streptococcus mutans

1990

Russel, R.R.B.; Ferretti, J.J.

J. Gen. Microbiol.

136

803-810

208778

7504068

Metabolism of polysaccharides by the Streptococcus mutans dexB gene product

1993

Whiting, G.C.; Sutcliffe, I.C.; Russel, R.R.B.

J. Gen. Microbiol.

139

2019-2026

289127

1537846

A binding protein-dependent transport system in Streptococcus mutans responsible for multiple sugar metabolism

1992

Russell, R.R.B.; Aduse-Opoku, J.; Sutcliffe, I.C.; Tao, L.; Ferretti, J.J.

J. Biol. Chem.

267

4631-4637

326246

3089779

Purification and characterization of Bacillus coagulans oligo-1,6-glucosidase

1986

Suzuki, Y.; Tomura, Y.

Eur. J. Biochem.

158

77-83

326251

-

Assignment of a p-nitrophenyl-alpha-D-glucopyranoside hydrolyzing alpha-glucosidase of Bacillus cereus ATCC 7064 to an exo-oligo-1,6-glucosidase

1982

Suzuki, Y.; Aoki, R.; Hayashi, H.

Biochim. Biophys. Acta

704

476-483

326254

365246

Hydrolysis of low molecular weight isomaltosaccharides by a p-nitrophenyl-alpha-D-glucopyranoside-hydrolyzing alpha-glucosidase from a thermophile, Bacillus thermoglucosidius KP 1006

1979

Suzuki, Y.; Ueda, Y.; Nakamura, N.; Abe, S.

Biochim. Biophys. Acta

566

62-66

326261

2346949

Purification and properties of an oligo-1,6-glucosidase from alkalophilic Bacillus species

1990

Yamamoto, M.; Horikoshi, K.

Carbohydr. Res.

197

227-235

393269

-

Thermostable extracellular alpha-amylase and alpha-glucosidase of Lipomyces starkeyi

1985

Kelly, C.T.; Moriarty, M.E.; Fogarty, W.M.

Appl. Microbiol. Biotechnol.

22

352-358

393270

-

Separation and characterization of an alpha-glucosidase and maltase from Bacillus amyloliquefaciens

1985

Fogarty, W.M.; Kelly, C.T.; Kadam, S.K.

Can. J. Microbiol.

31

670-674

393280

-

Extracellular alpha-glucosidase of an alkalophilic microorganism, Bacillus sp. ATCC 21591

1983

Kelly, C.T.; O'Reilly, F.; Fogarty, W.M.

FEMS Microbiol. Lett.

20

55-59

393292

339955

alpha-Glucosidase, a membrane-bound enzyme of alpha-glucan metabolism in Bacillus amyloliquefaciens. Purification and partial characterization

1978

Urlaub, H.; Woeber, G.

Biochim. Biophys. Acta

522

161-173

393298

-

Purification and properties of alpha-glucosidase from Penicillium oxalicum

1977

Yamasaki, Y.; Suzuki, Y.; Ozawa, J.

Agric. Biol. Chem.

41

1451-1458

393345

7785860

Transglucosylation of a fungal alpha-glucosidase. The enzyme properties and correlation of isomaltooligosaccharide production

1995

Duan, K.J.; Sheu, D.C.; Lin, C.T.

Ann. N. Y. Acad. Sci.

750

325-328

393346

7764968

Purification and characterization of a Bacillus sp. SAM1606 thermostable alpha-glucosidase with transglucosylation activity

1994

Nakao, M.; Nakayama, T.; Harada, M.; Kakudo, A.; Ikemoto, H.; Kobayashi, S.; Shibano, Y.

Appl. Microbiol. Biotechnol.

41

337-343

393347

-

Characterization of thermostable alpha-glucosidase from Clostridium thermosulfuricum 39E

1991

Saha, B.C.; Zeikus, J.G.

Appl. Microbiol. Biotechnol.

35

568-571

393353

1708385

Characterization of a neopullulanase and an alpha-glucosidase from Bacteroides thetaiotaomicron 95-1

1991

Smith, K.A.; Salyers, A.A.

J. Bacteriol.

173

2962-2968

654304

12658515

Cloning and characterization of two alpha-glucosidases from Bifidobacterium adolescentis DSM20083

2003

van den Broek, L.A.M.; Struijs, K.; Verdoes, J.C.; Beldman, G.; Voragen, A.G.J.

Appl. Microbiol. Biotechnol.

61

55-60

654806

11150611

Glucoamylase: structure/function relationships, and protein engineering

2000

Sauer, J.; Sigurskjold, B.W.; Christensen, U.; Frandsen, T.P.; Mirgorodskaya, E.; Harrison, M.; Roepstorff, P.; Svensson, B.

Biochim. Biophys. Acta

1543

275-293

655808

-

Evidence for a single active site on isomalto-dextranase with hydrolysis activities of alpha-1,6- and alpha-1,4-glucosidic linkages

2001

Takayanagi, T.; Kimura, A.; Matsui, H.; Okada, G.; Chiba, S.

J. Appl. Glycosci.

48

55-61

655809

-

Evaluation of subsite affinities of isomalto-dextranase from Arthrobacter globiformis

2002

Takayanagi, T.; Kimura, A.; Matsui, H.

J. Appl. Glycosci.

49

123-127

663981

16509822

The family 21 carbohydrate-binding module of glucoamylase from Rhizopus oryzae consists of two sites playing distinct roles in ligand binding

2006

Chou, W.I.; Pai, T.W.; Liu, S.H.; Hsiung, B.K.; Chang, M.D.

Biochem. J.

396

469-477

664233

16503208

Structural elements in dextran glucosidase responsible for high specificity to long chain substrate

2006

Saburi, W.; Mori, H.; Saito, S.; Okuyama, M.; Kimura, A.

Biochim. Biophys. Acta

1764

688-698

674950

16781466

Acceptor specificity of trehalose phosphorylase from Thermoanaerobacter brockii: production of novel nonreducing trisaccharide, 6-O-alpha-D-galactopyranosyl trehalose

2006

Maruta, K.; Watanabe, H.; Nishimoto, T.; Kubota, M.; Chaen, H.; Fukuda, S.; Kurimoto, M.; Tsujisaka, Y.

J. Biosci. Bioeng.

101

385-390

679550

-

Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli

2006

Lee, J.; Yoon, S.; Nam, S.; Moon, Y.; Moon, Y.; Kim, D.

Enzyme Microb. Technol.

39

612-620

690556

19114534

Characterization of two novel alpha-glucosidases from Bifidobacterium breve UCC2003

2009

Pokusaeva, K.; OConnell-Motherway, M.; Zomer, A.; Fitzgerald, G.F.; van Sinderen, D.

Appl. Environ. Microbiol.

75

1135-1143

692850

18223075

Molecular and physiological role of the trehalose-hydrolyzing alpha-glucosidase from Thermus thermophilus HB27

2008

Alarico, S.; da Costa, M.S.; Empadinhas, N.

J. Bacteriol.

190

2298-2305

699532

18395742

Substrate recognition mechanism of alpha-1,6-glucosidic linkage hydrolyzing enzyme, dextran glucosidase from Streptococcus mutans

2008

Hondoh, H.; Saburi, W.; Mori, H.; Okuyama, M.; Nakada, T.; Matsuura, Y.; Kimura, A.

J. Mol. Biol.

378

913-922

729747

24649402

Similarities and differences in the biochemical and enzymological properties of the four isomaltases from Saccharomyces cerevisiae

2014

Deng, X.; Petitjean, M.; Teste, M.; Kooli, W.; Tranier, S.; Francois, J.; Parrou, J.

FEBS Open Bio

4

200-212

732004

22685275

Enzymology and structure of the GH13_31 glucan 1,6-alpha-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM

2012

Moeller, M.S.; Fredslund, F.; Majumder, A.; Nakai, H.; Poulsen, J.C.; Lo Leggio, L.; Svensson, B.; Abou Hachem, M.

J. Bacteriol.

194

4249-4259

742891

27302067

Two novel glycoside hydrolases responsible for the catabolism of cyclobis-(1->6)-alpha-nigerosyl

2016

Tagami, T.; Miyano, E.; Sadahiro, J.; Okuyama, M.; Iwasaki, T.; Kimura, A.

J. Biol. Chem.

291

16438-16447

746984

28042032

Characterization of divergent pseudo-sucrose isomerase from Azotobacter vinelandii Deciphering the absence of sucrose isomerase activity

2017

Jung, J.H.; Kim, M.J.; Jeong, W.S.; Seo, D.H.; Ha, S.J.; Kim, Y.W.; Park, C.S.

Biochem. Biophys. Res. Commun.

483

115-121

Links to other databases for isomaltotriose

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ChEBI

PubChem