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Ligand poly(3-hydroxybutanoate)n

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Natural Substrate (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.1.1.75

poly(3-hydroxybutyrate) + H2O = ?

716045, 713926, 729031, 730415, 728941

-

3.1.1.76

poly(3-hydroxybutyrate) + H2O = ?

729031

-

3.1.1.76

poly(3-hydroxybutyrate) + H2O = oligo(3-hydroxybutyrate)

665202, 664975

-

3.1.1.76

2 entries

Substrate in Enzyme-catalyzed Reactions (11 results)

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Show Substrate (11 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.30

poly-3-hydroxybutyrate + NAD+ = acetoacetate + NADH + H+

695653

-

3.1.1.75

poly (3-hydroxybutanoate) n + n H2O = poly (3-hydroxybutanoate) n-1 + (R)-3-hydroxybutanoate

752076

-

3.1.1.75

poly (3-hydroxybutyrate) + H2O = ?

694834, 701454

-

3.1.1.75

poly(3-hydroxybutyrate) + H2O = 3-hydroxybutyrate + 3-hydroxybutyryl-3-hydroxybutyrate

730744

-

3.1.1.75

poly(3-hydroxybutyrate) + H2O = ?

729468, 716045, 713926, 729031, 690485, 693659, 730415, 728941, 729044, 701802

-

3.1.1.75

poly(3-hydroxybutyric acid) + H2O = ?

208341, 208351, 208342, 208343, 680988, 208348

-

3.1.1.75

poly-3-hydroxybutyrate + H2O = ?

702902

-

3.1.1.75

polyhydroxybutyrate + H2O = 3-hydroxybutyrate + ?

690603

-

3.1.1.76

poly(3-hydroxybutyrate) + H2O = ?

729031

-

3.1.1.76

poly(3-hydroxybutyrate) + H2O = oligo(3-hydroxybutyrate)

665202, 664294, 664975

-

3.1.1.76

poly(3-hydroxybutyrate) + H2O = oligo(3-hydroxybutyrate) + D-(-)-3-hydroxybutyrate dimer

664975

-

3.1.1.75

7 entries

3.1.1.76

3 entries

Product in Enzyme-catalyzed Reactions (2 results)

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Show Product (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.304

3-hydroxybutyryl-CoA + [3-hydroxybutanoate]n = [3-hydroxybutanoate](n+1) + CoA

-

-

2.3.1.304

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n = [3-hydroxybutyrate](n+1) + CoA

-

-

2.3.1.304

2 entries

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.14.13.25

MMO activity dramatically decreases when cellular poly-beta-hydroxybutyrate accumulates in the second stage. The more poly-beta-hydroxybutyrate are accumulated, the slower MMO activity decreases. Cellular poly-beta-hydroxybutyrate content has some influence on the maintenance of the MMO activity

701759

-

Enzyme Kinetic Parameters

K_M Value (7 results)

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Show KM Value (7 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.1.1.75

-999

-

0.018 mg/ml

208348

3.1.1.75

-999

-

0.018 mg/ml, isoform E1, 0.07 mg/ml, isoform E2

208351

3.1.1.75

-999

-

0.06 mg/ml, P(3HB) depolymerase B, 0.1 mg/ml, P(3HB) depolymerase A

208342

3.1.1.75

-999

-

0.17 mg/ml

208343

3.1.1.75

-999

-

0.27mg/ml, in 200 mM NaCl

208341

3.1.1.75

-999

-

values in mg/ml

208341, 208342

3.1.1.75

6 entries

3.1.1.76

2.8

-

pH 7.9, 30°C

665202

References & Links

Literature References (27)

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Show Reference (27 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

208341

7646009

Properties of poly(3-hydroxybutyrate) depolymerase from a marine bacterium, Alcaligenes faecalis AE122

1995

Kita, K.; Ishimaru, K.; Teraoka, M.; Yanase, H.; Kato, N.

Appl. Environ. Microbiol.

61

1727-1730

208342

-

Purification and properties of poly(3-hydroxyvaleric acid) depolymerase from Pseudomonas lemoignei

1993

Muller, B.; Jendrossek, D.

Appl. Microbiol. Biotechnol.

37-8

487-492

208343

1929416

Extracellular poly(3-hydroxybutyrate) depolymerase from Penicillium funiculosum: General characteristics and active site studies

1991

Brucato, C.L.; Wong, S.S.

Arch. Biochem. Biophys.

290

497-502

208348

-

Thermostable poly(3-hydroxybutyrate) depolymerase of a thermophilic strain of Leptothrix sp. isolated from a hot spring

1998

Takeda, M.; Koizumi, J.I.; Yabe, K.; Adachi, K.

J. Ferment. Bioeng.

85

375-380

208351

-

Purification and characterization of extracellular poly(3-hydroxybutyrate) depolymerases produced by Agrobacterium sp. K-03

1996

Nojima, S.; Mineki, S.; Iida, M.

J. Ferment. Bioeng.

81

72-75

664294

12099829

Enzymatic hydrolysis of bacterial poly(3-hydroxybutyrate-co-3-hydroxypropionate)s by poly(3-hydroxyalkanoate) depolymerase from Acidovorax sp. TP4

2002

Wang, Y.; Inagawa, Y.; Saito, T.; Kasuya, K.i.; Doi, Y.; Inoue, Y.

Biomacromolecules

3

828-834

664975

11267773

Identification of the intracellular polyhydroxyalkanoate depolymerase gene of Paracoccus denitrificans and some properties of the gene product

2001

Gao, D.; Maehara, A.; Yamane, T.; Ueda, S.

FEMS Microbiol. Lett.

196

159-164

665202

-

Polyhydroxyalkanoate degrading hydrolase-like activities by Pseudomonas sp. isolated from soil

2004

Colak, A.; Güner, S.

Int. Biodeter. Biodegrad.

53

103-109

680988

17543409

Studies on intracellular degradation of polyhydroxyalkanoic acid-polyethylene glycol copolymer accumulated by Azotobacter chroococcum MAL-201

2007

Saha, S.P.; Patra, A.; Paul, A.K.

J. Biotechnol.

132

325-330

690485

18340545

Secretion pathway for the poly(3-hydroxybutyrate) depolymerase in Ralstonia pickettii T1

2008

Sugimoto, A.; Shiraki, M.; Hatakeyama, S.; Saito, T.

Antonie van Leeuwenhoek

94

223-232

690603

19214501

An extracellular polyhydroxybutyrate depolymerase in Thermus thermophilus HB8

2009

Papaneophytou, C.P.; Pantazaki, A.A.; Kyriakidis, D.A.

Appl. Microbiol. Biotechnol.

83

659-668

693659

18706425

Structural basis of poly(3-hydroxybutyrate) hydrolysis by PhaZ7 depolymerase from Paucimonas lemoignei

2008

Papageorgiou, A.C.; Hermawan, S.; Singh, C.B.; Jendrossek, D.

J. Mol. Biol.

382

1184-1194

694834

-

Purification and characterization of extracellular poly(beta -hydroxybutyrate) depolymerase from Penicillium sp. DS9701-D2

2009

Zhou, H.; Wang, Z.; Chen, S.; Liu, D.; Xia, H.

Polym. -Plast. Technol. Eng.

48

58-63

695653

19219638

Characterization of a novel 3-hydroxybutyrate dehydrogenase from Ralstonia pickettii T1

2009

Takanashi, M.; Shiraki, M.; Saito, T.

Antonie van Leeuwenhoek

95

249-262

701454

19133504

A novel poly(3-hydroxybutyrate)-degrading Streptoverticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase

2008

Shah, A.A.; Hasan, F.; Hameed, A.; Ahmed, S.

Acta Biol. Hung.

59

489-499

701759

19052919

The methane monooxygenase intrinsic activity of kinds of methanotrophs

2009

Zhang, Y.; Xin, J.; Chen, L.; Xia, C.

Appl. Biochem. Biotechnol.

157

431-441

701802

19561190

Identification and characterization of a novel intracellular poly(3-hydroxybutyrate) depolymerase from Bacillus megaterium

2009

Chen, H.J.; Pan, S.C.; Shaw, G.C.

Appl. Environ. Microbiol.

75

5290-5299

702902

20346169

Identification and characterization of the intracellular poly-3-hydroxybutyrate depolymerase enzyme PhaZ of Sinorhizobium meliloti

2010

Trainer, M.A.; Capstick, D.; Zachertowska, A.; Lam, K.N.; Clark, S.R.; Charles, T.C.

BMC Microbiol.

10

92

713926

21845385

Extracellular production of Streptomyces exfoliatus poly(3-hydroxybutyrate) depolymerase in Rhodococcus sp. T104: determination of optimal biocatalyst conditions

2012

Garcia-Hidalgo, J.; Hormigo, D.; Prieto, M.A.; Arroyo, M.; de la Mata, I.

Appl. Microbiol. Biotechnol.

93

1975-1988

716045

-

Purification and properties of extracellular poly(3-hydroxybutyrate) depolymerase produced by Aspergillus fumigatus 202

2010

Bhatt, R.; Patel, K.; Trivedi, U.

J. Polym. Environ.

18

141-147

728941

22537946

Examination of PHB depolymerases in Ralstonia eutropha: further elucidation of the roles of enzymes in PHB homeostasis

2012

Brigham, C.J.; Reimer, E.N.; Rha, C.; Sinskey, A.J.

AMB Express

2

26

729031

22865072

Characterization of a novel subgroup of extracellular medium-chain-length polyhydroxyalkanoate depolymerases from actinobacteria

2012

Gangoiti, J.; Santos, M.; Prieto, M.A.; de la Mata, I.; Serra, J.L.; Llama, M.J.

Appl. Environ. Microbiol.

78

7229-7237

729044

24907326

To be or not to be a poly(3-hydroxybutyrate) (PHB) depolymerase: PhaZd1 (PhaZ6) and PhaZd2 (PhaZ7) of Ralstonia eutropha, highly active PHB depolymerases with no detectable role in mobilization of accumulated PHB

2014

Sznajder, A.; Jendrossek, D.

Appl. Environ. Microbiol.

80

4936-4946

729468

100020549

Purification and characterization of two extracellular polyhydroxyalkanoate depolymerases from Pseudomonas mendocina

2013

Mao, H.; Jiang, H.; Su, T.; Wang, Z.

Biotechnol. Lett.

35

1919-1924

730415

24007310

Biochemical analysis and structure determination of Paucimonas lemoignei poly(3-hydroxybutyrate) (PHB) depolymerase PhaZ7 muteins reveal the PHB binding site and details of substrate-enzyme interactions

2013

Jendrossek, D.; Hermawan, S.; Subedi, B.; Papageorgiou, A.C.

Mol. Microbiol.

90

649-664

730744

23951224

Novel extracellular PHB depolymerase from Streptomyces ascomycinicus: PHB copolymers degradation in acidic conditions

2013

Garcia-Hidalgo, J.; Hormigo, D.; Arroyo, M.; de la Mata, I.

PLoS ONE

8

e71699

752076

-

Efficient production of (R)-3-hydroxybutyric acid by Pseudomonas sp. DS1001a and its extracellular poly(3-hydroxybutyrate) depolymerase

2016

Li, F.; Zhang, C.; Liu, Y.; Liu, D.; Xia, H.; Chen, S.

Process Biochem.

51

369-373

Links to other databases for poly(3-hydroxybutanoate)n

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