Ligand dTTP

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Basic Ligand Information

Molecular Structure

C₁₀H₁₇N₂O₁₄P₃

dTTP

NHVNXKFIZYSCEB-XLPZGREQSA-N

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Synonyms:

2'-deoxy-beta-L-thymidine-5'-triphosphate, 2'-deoxythymidine 5'-triphosphate, 2'-dTTP, 5'-dTTP, D-beta-deoxythymidine-triphosphate, deoxythymidine 5'-triphosphate, thymidine 5'-triphosphate, thymidine triphosphate, TTP

Related pathways

BRENDA

dTDPLrhamnose biosynthesis, macrolide antibiotic biosynthesis, pyrimidine metabolism

KEGG

Nucleotide metabolism, Pyrimidine metabolism

MetaCyc

dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose biosynthesis, dTDP-3-acetamido-alpha-D-fucose biosynthesis, dTDP-4-O-demethyl-beta-L-noviose biosynthesis, dTDP-6-deoxy-alpha-D-allose biosynthesis, dTDP-alpha-D-forosamine biosynthesis

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Show all BRENDA pathways known for dTTP

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (23 results)

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2.7.7.24

14 entries

2.7.7.64

TTP + alpha-D-glucose 1-phosphate = diphosphate + TDP-D-glucose

721524

2.7.7.9

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose

dTTP + H2O = dTDP + phosphate

210044, 210046, 210049, 210045, 210043, 210047, 210048, 210050, 210051, 688933, 697608

3.6.1.5

TTP + 2 H2O = TMP + 2 phosphate

700083

5.6.2.4

wound dsDNA + dTTP + H2O = unwound ssDNA + dTDP + phosphate

768319

5.6.2.6

2 entries

In Vivo Product in Enzyme-catalyzed Reactions (12 results)

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2.7.1.40

TDP + phosphoenolpyruvate = TTP + pyruvate

2.7.4.1

TDP + (phosphate)n+1 = TTP + (phosphate)n

645125

2.7.4.3

ADP + TDP = AMP + TTP

Substrate in Enzyme-catalyzed Reactions (848 results)

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1.1.98.6

TTP + formate = dTTP + CO2 + H2O

740665

2.6.99.1

dTTP + depurinated DNA = deoxyribose triphosphate + purinated DNA

640205

2.7.1.1

TTP + D-glucose = TDP + D-glucose 6-phosphate

676958, 739403

2.7.1.11

TTP + D-fructose 6-phosphate = TDP + D-fructose 1,6-bisphosphate

TTP + D-tagatose 6-phosphate = TDP + D-tagatose 1,6-bisphosphate

640993

2.7.1.165

TTP + (R)-glycerate = TDP + 2-phospho-(R)-glycerate

685823

2.7.1.20

TTP + adenosine = TDP + AMP

TTP + 3'-dephospho-CoA = TDP + CoA

759246

2.7.1.30

TTP + glycerol = TDP + glycerol 3-phosphate

641289

2.7.1.33

pantothenate + dTTP = 4'-phosphopantothenate + dTDP

TTP + D-fructose = TDP + D-fructose 6-phosphate

641501, 662020

2.7.1.45

TTP + 2-dehydro-3-deoxy-D-gluconate = TDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate

676988

2.7.1.48

dTTP + uridine = dTDP + UMP

641643

2.7.1.50

TTP + 4-methyl-5-(2-hydroxyethyl)thiazole = TDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole

737878

2.7.1.56

TTP + D-fructose 1-phosphate = TDP + D-fructose 1,6-bisphosphate

640483, 727057

2.7.1.6

dTTP + D-galactose = ?

721979

2.7.1.63

TTP + glucose = TDP + D-glucose 6-phosphate

641755

2.7.1.67

TTP + 1-phosphatidyl-1D-myo-inositol = TDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate

641787

2.7.1.7

TTP + D-mannose = TDP + D-mannose 6-phosphate

TTP + 5'-dephospho-DNA = TDP + 5'-phospho-DNA

642004

2.7.1.B20

TTP + inosine = TDP + inosine 5'-phosphate

697882

2.7.2.1

TTP + acetate = TDP + acetyl phosphate

642184, 642178, 727804, 730304

2.7.2.3

2'-dTTP + 3-phospho-D-glycerate = 2'-dTDP + 3-phospho-D-glyceroyl phosphate

TTP + (R,S)-5-phosphomevalonate = TDP + (R,S)-5-diphosphomevalonate

dTTP + dTMP = dTDP

dTTP + UMP = dTDP + UDP

663751

2.7.4.B7

dTTP + (phosphate)n = dTDP + (phosphate)n+1

762661

2.7.7.13

dTTP + alpha-D-mannose 1-phosphate = alpha-dTDP-mannose + diphosphate

dTTP + alpha-D-galactose 1-phosphate = dTDPgalactose + ?

dTTP + tobramycin = diphosphate + 2''-deoxythymidylyltobramycin

dTTP + RNAn = ?

dTTP + valienol 1-phosphate = diphosphate + dTDP-valienol

dTTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + dTDP-N-acetyl-alpha-D-glucosamine

TTP + H2O = ?

thymidine 5'-triphosphate + H2O = ?

208385

3.1.5.B1

dTTP + H2O = deoxythymidine + triphosphate

730044, 749906, 771379, 772963

3.6.1.1

TTP + H2O = ? + phosphate

677801

3.6.1.12

dTTP + H2O = dTMP + diphosphate

722230, 734816, 733297

3.6.1.15

3 entries

3.6.1.23

dTTP + H2O = dTMP + diphosphate

209996, 209998, 209990, 209997, 657286, 648212, 656212, 711327, 756316

dTTP + H2O = ?

dTTP + H2O = dTMP + diphosphate

TTP + oxaloacetate = TDP + phosphoenolpyruvate + CO2

4636

5.6.1.2

TTP + H2O + a dynein associated with a microtubule at position n = TDP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)

247009, 247007

5.6.1.5

TTP + H2O + polypeptide = TDP + phosphate + unfolded polypeptide

TTP + H2O + actin = TDP + phosphate + actin

654976

5.6.2.3

dTTP + H2O = dTDP + phosphate

dTTP + H2O + wound DNA = dTDP + phosphate + unwound DNA

775949

6.2.1.22

dTTP + acetate + citrate (pro-3S)-lyase = dTMP + diphosphate + citrate(pro-3S)-lyase

697

6.2.1.26

TTP + o-succinylbenzoate + CoA = TMP + diphosphate + o-succinylbenzoyl-CoA

648943

6.3.1.2

TTP + L-Glu + NH4+ = TDP + phosphate + L-Gln

TTP + gamma-L-glutamyl-L-cysteine + glycine = TDP + phosphate + glutathione

775688

6.3.3.2

dTTP + 5-formyltetrahydrofolate = dTDP + phosphate + 5,10-methylenetetrahydrofolate

1322

6.3.3.3

TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 = TDP + phosphate + dethiobiotin + Na+ + OH-

746545

6CZC, 3D-view

6.5.1.1

ATP + dTTP = P1-(5'-adenosyl),P4-(5'-thymidyl)tetraphosphate + diphosphate

722217

6.5.1.6

TTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = TMP + diphosphate + (deoxyribonucleotide)n+m

672745

Product in Enzyme-catalyzed Reactions (22 results)

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1.1.98.6

TTP + formate = dTTP + CO2 + H2O

2.7.1.40

2 entries

2.7.3.8

TDP + phosphoramide = TTP + NH3

2.7.4.1

TDP + (phosphate)n+1 = TTP + (phosphate)n

645125

2.7.4.3

ADP + TDP = AMP + TTP

2.7.4.6

3 entries

2.7.4.B7

dTDP + (phosphate)n+1 = dTTP + (phosphate)n

2.7.7.24

7 entries

2.7.7.9

diphosphate + TDP-glucose = TTP + alpha-D-glucose 1-phosphate

643736, 643748, 643751, 643743, -

3.5.4.13

4 entries

Enzyme Cofactor/Cosubstrate (15 results)

can partly substitute for ATP

775949

6.3.4.21

2 entries

Activator in Enzyme-catalyzed Reactions (416 results)

stimulation, cytosolic isozyme I

less effective than ATP

low activating effect

727105

Inhibitor in Enzyme-catalyzed Reactions (406 results)

2 mM, 71% inhibition of NADP+ reduction

poor

10 mM, strong

5 mM, 12% inhibition

1 mM, 29% inhibition

1 mM, 13% inhibition

allosteric inhibition

0.1 mM, 30% inhibition

with ATP as substrate

636985

2.7.1.52

4.8 mM, 45% inhibition in presence of ATP

more than 95% inhibition at 0.3 mM

642023

2.7.4.14

inhibits reaction with ATP and UMP, CMP or dCMP

47% inhibition at 1 mM

strong, competitive with CTP, kinetics

643238

2.7.7.43

10 mM in presence of 3 mM CTP, 71% inhibition

IC50 above 0.5 mM

inhibits uridylyl removing activity

competitive inhibitor

competitive to ATP

competitive

70% inhibition at 1 mM

246829

4.1.1.35

0.2 mM, 96% inhibition; 0.2 mM, 97% inhibition

97% and 99% inhibition of RNA and DNA helicase activity at 2.5 mM, respectively

769158

5.6.2.4

inhibition of NTPase activity of NS3 protein by NTP derivatives

696161

5.6.2.5

97% and 99% inhibition of RNA and DNA helicase activity at 2.5 mM, respectively

769158

5.6.2.6

inhibition of NTPase activity of NS3 protein by NTP derivatives

less than 40% residual activity at 20 mM

727879

6.3.4.16

0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C

722163

6.3.4.5

slight

1576

3D Structure of Enzyme-Ligand-Complex (PDB) (1666 results)

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (112 results)

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2.7.1.1

1.42

30°C, pH 8.0

676958

2.7.1.20

0.3

at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol

0.4

1.64

pH 7.6, 37°C, recombinant enzyme

107

3.68

pH and temperature not specified in the publication

11.8

wild-type, pH 7.5, 37°C

746545

K_M Value (167 results)

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1.17.4.1

0.0015

positive effector of CDP reduction

437939

2.7.1.1

0.231

30°C, pH 8.0

0.245

at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol

0.723

1.9

pH 10, 37°C

pH 7.5, 25°C

0.001

cosubstrate 2'-deoxycytidine, 37°C, pH 7.4

8.1

pH 9.0, 37°C, reaction with N-acetylneuraminate

0.0004

0.299

1.23

pH and temperature not specified in the publication

0.25

pH 7.6, 25°C

0.0066

0.023

wild-type, pH 7.5, 37°C

746545

K_i Value (27 results)

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1.1.98.6

0.05

substrate GTP, pH not specified in the publication, temperature not specified in the publication

0.04

pH 8.5, 37°C

643238

2.7.8.11

1.4

pH 9.0, 37°C

645311

3.5.4.13

0.1

pH 6.8, 25°C

652395

3.6.1.15

2.8

pH 7.5, 25°C, inhibition of ATPase activity

656494

4.2.1.46

0.00567

pH 7.5, 30°C

746731

5.6.2.4

0.116

inhibition of NTPase activity of NS3 protein by NTP derivatives

696161

5.6.2.6

0.116

inhibition of NTPase activity of NS3 protein by NTP derivatives

696161

IC₅₀ Value (6 results)

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2.7.1.145

0.012

IC50: 0.012 mM, reaction with thymidine, fusion protein with glutathione S-transferase

636843

2.7.1.21

0.0038

Hill coefficient 1.6, pH 7.4, 37°C

739404

2.7.4.22

0.4

wild type enzyme, at pH 7.4 and 30°C

723289

2.7.7.48

0.5

IC50 above 0.5 mM

643393

4.2.1.46

0.032

pH 7.5, 30°C

746731

6.3.3.3

0.622

wild-type, pH 7.5, 37°C

746545

References & Links

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Show Reference (448 entries)

Links to other databases for dTTP

Ligand dTTP

Basic Ligand Information

Related pathways

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (23 results)

In Vivo Product in Enzyme-catalyzed Reactions (12 results)

Substrate in Enzyme-catalyzed Reactions (848 results)

Product in Enzyme-catalyzed Reactions (22 results)

Enzyme Cofactor/Cosubstrate (15 results)

Activator in Enzyme-catalyzed Reactions (416 results)

Inhibitor in Enzyme-catalyzed Reactions (406 results)

3D Structure of Enzyme-Ligand-Complex (PDB) (1666 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (112 results)

KM Value (167 results)

Ki Value (27 results)

IC50 Value (6 results)

References & Links

Literature References (448)

Links to other databases for dTTP

k_cat Value (Turnover Number) (112 results)

K_M Value (167 results)

K_i Value (27 results)

IC₅₀ Value (6 results)