Ligand dATP

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Basic Ligand Information

Molecular Structure

C₁₀H₁₆N₅O₁₂P₃

dATP

SUYVUBYJARFZHO-RRKCRQDMSA-N

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Synonyms:

2'-dATP, 2'-deoxy-adenosine 5'-triphosphate, 2'-deoxy-ATP, 2'-deoxyadenosine-3'-triphosphate, 2'-deoxyadenosine 5'-triphosphate, 2'-deoxyATP, 2-dATP, 2-deoxyadenosine 5'-triphosphate, 2-DeoxyATP, 5'-dATP, Deoxy-ATP, deoxyadenosine 5'-triphosphate, deoxyATP

Related pathways

BRENDA

purine metabolism

KEGG

Nucleotide metabolism, Purine metabolism

MetaCyc

adenosine deoxyribonucleotides de novo biosynthesis I, adenosine deoxyribonucleotides de novo biosynthesis II, purine deoxyribonucleosides salvage

Show all BRENDA pathways known for dATP

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (14 results)

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2.5.1.17

cob(I)alamin + dATP + H2O + H+ = deoxyadenosylcobalamin + diphosphate + phosphate

674576

2.7.1.33

dATP + (R)-pantothenate = dADP + (R)-4'-phosphopantothenate

dATP + H2O = dADP + phosphate

684774, 696275, 757096

3.6.1.9

dATP + H2O = ?

246817

5.6.2.4

wound dsDNA + dATP + H2O = unwound ssDNA + dADP + phosphate

768319, 775695

5.6.2.5

wound dsRNA + dATP + H2O = unwound RNA + dADP + phosphate

dATP + acetate + CoA = dAMP + diphosphate + acetyl-CoA

652101

6.5.1.1

dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = dAMP + diphosphate + (deoxyribonucleotide)m+n

662058

In Vivo Product in Enzyme-catalyzed Reactions (23 results)

dADP + (phosphate)n+1 = dATP + (phosphate)n

645125

Substrate in Enzyme-catalyzed Reactions (674 results)

peroxiredoxin-(S-hydroxy-S-oxocysteine) + dATP + 2 R-SH = peroxiredoxin-(S-hydroxycysteine) + dADP + phosphate + R-S-S-R

659421

2.3.3.8

dATP + citrate + CoA = dADP + phosphate + acetyl-CoA + oxaloacetate

488211, 488225

2.5.1.112

dimethylallyl diphosphate + dATP = ?

dimethylallyl diphosphate + dATP = diphosphate + N6-(dimethylallyl)dATP

671796

2.5.1.6

2'-deoxy-ATP + L-methionine + H2O = ?

639074, 639047, 639045, 639049, 639056, 639041, 639052, 639039, 639042, 639043, 639044, 639053, 639054, 639058, 639051, 639055, 639057, 639038, 639040, 639048, 639046, 639050

2.6.99.1

2 entries

2.7.1.1

2'-dATP + D-glucose = 2'-dADP + D-glucose 6-phosphate

640226, 640209, 640208

2.7.1.100

dATP + 5-methylthioribose = dADP + 5-methylthioribose 1-phosphate

640268

2.7.1.107

2'-deoxy-ATP + sn-1,2-dihexanoylglycerol = 2'-deoxy-ADP + sn-1,2-dihexanoylglycerol 3-phosphate

640387

2.7.1.11

dATP + D-fructose 6-phosphate = dADP + D-fructose 1,6-bisphosphate

640421, 640463, 640486

2.7.1.113

2 entries

2.7.1.15

dATP + D-ribose = dADP + D-ribose 5-phosphate

641000

2.7.1.156

dATP + adenosylcobinamide = adenosylcobinamide phosphate + dADP

644438

2.7.1.164

dATP + L-seryl-tRNASec = dADP + O-phospho-L-seryl-tRNASec

694429

2.7.1.229

dATP + 2-deoxy-D-ribose = dADP + 2-deoxy-D-ribose 5-phosphate

dATP + adenosine 5-phosphosulfate = dADP + 3'-phosphoadenosine 5'-phosphosulfate

dATP + mevalonate = dADP + phosphomevalonate

641436

2.7.1.4

dATP + D-fructose = dADP + D-fructose 6-phosphate

641483, 641497, 641507

2.7.1.43

dATP + D-glucuronate = dADP + 1-phospho-D-glucuronate

dATP + 4-methyl-5-(2-hydroxyethyl)thiazole = dADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole

639720, 737878

2.7.1.59

dATP + N-acetyl-D-glucosamine = dADP + N-acetyl-D-glucosamine 6-phosphate

dATP + glucose = dADP + D-glucose 6-phosphate

641750

2.7.1.65

2'-deoxy-ATP + scyllo-inosamine = 2'-deoxy-ADP + scyllo-inosamine phosphate

dATP + D-mannose = dADP + D-mannose 6-phosphate

641894

2.7.1.71

2'-deoxyATP + shikimate = 2'-deoxyADP + shikimate 3-phosphate

641904

2.7.1.72

dATP + streptomycin = dADP + streptomycin 6-phosphate

dATP + deoxycytidine = dADP + dCMP

dATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] = dADP + ?

640979

2.7.11.31

dATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = dADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate

644956

2.7.2.2

dATP + carbamate = dADP + carbamoyl phosphate

dATP + N-acetyl-L-glutamate = dADP + N-acetyl-L-glutamate 5-phosphate

2'-deoxy-adenosine 5'-triphosphate + trans-farnesyl diphosphate = 2'-deoxyadenosine 5'-diphosphate + trans-farnesyl triphosphate

645172

2.7.4.19

dATP + 5-methyldeoxycytidine 5'-phosphate = dADP + 5-methyldeoxycytidine diphosphate

642548

2.7.4.25

dATP + CMP = dADP + CDP

dATP + dTMP = dADP + dTDP

645188, 645189, 645196, 645201, 645203, 645191, 645193, 645198, 695005, 692915, 737398

2.7.4.B1

dATP + UMP = dADP + UDP

663751

2.7.6.1

dATP + D-ribose 5-phosphate = dAMP + 5-phospho-alpha-D-ribose 1-diphosphate

642710, 642712, 642729, 642738, 642716, 728136, 738194

2.7.6.3

dATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = dAMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine

dATP + GTP = dAMP + guanosine 3'-diphosphate 5'-triphosphate

dATP + RNA = diphosphate + ?

2'-deoxy-ATP + pantetheine 4'-phosphate = ?

dATP + SO42- = diphosphate + deoxyadenylylsulfate

643261, 643281, 643292

2.7.7.46

dATP + tobramycin = diphosphate + 2''-deoxyadenylyltobramycin

645286

2.7.7.48

dATP + RNAn = diphosphate + RNAn+1

2'-deoxy-ATP + RNAn = diphosphate + RNAn+1

p-tRNAHis + dATP + GTP = pppGp-tRNAHis + dAMP + diphosphate

dATP + alpha-D-glucose 1-phosphate = diphosphate + dADP-alpha-D-glucose

662454

2.7.9.1

2'-dATP + pyruvate + phosphate = ?

dATP + 3 H2O = 2'-deoxyadenosine + 3 phosphate

dATP + H2O = ?

209371, 209380, 209381

3.5.4.17

dATP + H2O = dITP + NH3

246706

3.5.4.18

dATP + H2O = dITP + NH3

246717, 172176

3.6.1.1

dATP + H2O = dADP + phosphate

712033

3.6.1.10

dATP + H2O = dADP + phosphate

756125

3.6.1.12

dATP + H2O = dAMP + diphosphate

722230, 734816, 733297

3.6.1.15

dATP + H2O = dADP + phosphate

656494, 670074, 667694, 684774, 696275, 757096

dATP + H2O = ?

dATP + H2O = ?

dATP + H2O = dAMP + diphosphate

657286, 711327, 756316

3.6.1.5

3 entries

3.6.1.52

dATP + H2O = dADP + phosphate

393824

3.6.1.55

dATP + H2O = dAMP + diphosphate

756066

3.6.1.56

dATP + H2O = dAMP + diphosphate

757347

3.6.1.65

dATP + H2O = dAMP + diphosphate

756613

3.6.1.66

dATP + H2O = dAMP + diphosphate

778776

3.6.1.67

dATP + H2O = dAMP + diphosphate

728808

3.6.1.8

dATP + H2O = dAMP + diphosphate

dATP + 5-diphosphomevalonate = dADP + phosphate + isopentenyl diphosphate + CO2

4377

4.6.1.1

dATP = deoxy-cAMP + diphosphate

34793

5.6.1.2

2'-dATP + H2O + a dynein associated with a microtubule at position n = 2'-dADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)

247036, 210563

5.6.1.4

2'-dATP + H2O + a kinesin associated with a microtubule at position n = 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)

210563

5.6.1.6

dATP + H2O + closed Cl- channel = dADP + phosphate + open Cl- channel

751114

5.6.2.2

dATP + negatively supercoiled circular DNA = dADP + phosphate + relaxed circular DNA

3813, 3859

5.6.2.3

dATP + H2O = dADP + phosphate

656166, 690795, 692898, 690869, 692936, 692937, 694414, 756304, 692828

5.6.2.4

2 entries

5.6.2.5

wound dsRNA + dATP + H2O = unwound RNA + dADP + phosphate

dATP + H2O = dADP + phosphate

694539, 694692

5.6.2.B4

dATP + H2O + wound DNA = dADP + phosphate + unwound DNA

2'-deoxyadenosine 5'-triphosphate + L-arginine + tRNAArg = 2'-deoxyadenosine 5'-monophosphate + diphosphate + L-arginyl-tRNAArg

273, 280

6.1.1.20

2'-deoxyadenosine 5'-triphosphate + L-phenylalanine + tRNAPhe = 2'-deoxyadenosine 5'-monophosphate + diphosphate + L-phenylalanyl-tRNAPhe

340, 347

6.1.1.21

dATP + L-histidine + tRNAHis = dAMP + diphosphate + L-histidyl-tRNAHis

372

6.1.1.4

2'-dATP + L-leucine + tRNALeu = 2'-dAMP + diphosphate + L-leucyl-tRNALeu

442

6.1.1.6

dATP + lysine + tRNALys = dAMP + L-lysyl-tRNALys + diphosphate

483

6.2.1.1

dATP + acetate + CoA = dAMP + diphosphate + acetyl-CoA

652101, 563, 568

6.2.1.20

dATP + a fatty acid + [acyl-carrier protein] = dAMP + diphosphate + fatty acid-[acyl-carrier protein]

689

6.2.1.22

dATP + acetate + citrate (pro-3S)-lyase = dAMP + diphosphate + citrate(pro-3S)-lyase

695

6.2.1.3

dATP + fatty acid + CoA = dAMP + diphosphate + acyl-CoA

758, 764, 759

6.2.1.7

dATP + cholate + CoA = dAMP + diphosphate + choloyl-CoA

843

6.3.1.5

dATP + deamido-NAD+ + NH3 = dAMP + diphosphate + NAD+

953

6.3.2.12

dATP + 7,8-dihydropteroate + L-Glu = dADP + phosphate + 7,8-dihydrofolate

dATP + L-glutamate + L-cysteine = dADP + phosphate + gamma-L-glutamyl-L-cysteine

dATP + 5-formyltetrahydrofolate = dADP + phosphate + 5,10-methylenetetrahydrofolate

1322

6.3.4.2

deoxyATP + UTP + NH4+ = deoxyADP + phosphate + CTP

648982

6.3.4.3

dATP + formate + tetrahydrofolate = dADP + phosphate + 10-formyltetrahydrofolate

1506

6.3.4.5

dATP + L-citrulline + L-Asp = dAMP + diphosphate + L-argininosuccinate

1576

6.3.4.6

dATP + urea + HCO3- = ?

dATP + acetyl-CoA + HCO3- = dADP + phosphate + malonyl-CoA

dATP + (ribonucleotide)n + (ribonucleotide)m = dAMP + phosphate + (ribonucleotide)n+m

1985, 1984, 1997, 1995, 1910

6.6.1.2

dATP + hydrogenobyrinic acid a,c-diamide + Co2+ = dADP + phosphate + cob(II)yrinic acid a,c-diamide + H+

653078

7.1.2.1

dATP + H2O + H+/in = dADP + phosphate + H+/out

2'-deoxyATP + H2O + H+/in + K+/out = 2'-deoxyADP + phosphate + H+/out + K+/in

246853, 246851

7.6.2.1

dATP + H2O = dADP + H2O

246839, 246842

Product in Enzyme-catalyzed Reactions (37 results)

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1.1.98.6

ATP + formate = dATP + CO2 + H2O

1.14.11.33

1-methyl-dATP + 2-oxoglutarate + O2 = dATP + formaldehyde + succinate + CO2

dADP + phosphoenolpyruvate = dATP + pyruvate

2.7.3.5

dADP + phospholombricine = dATP + lombricine

2.7.3.8

dADP + phosphoramide = dATP + NH3

2'-dAMP + phosphoenolpyruvate + diphosphate = 2'-dATP + pyruvate + phosphate

3.6.1.17

2'-deoxyadenosine adenosine tetraphosphate + H2O = ATP + 2'-dAMP + 2'-dATP + AMP

209914

6.2.1.13

dADP + phosphate + acetyl-CoA = dATP + acetate + CoA

Enzyme Cofactor/Cosubstrate (32 results)

stimulates

activation, can replace ATP to some extent

485955

2.5.1.17

about 25% of the activity with ATP

activation

73% of the activity with ATP and deoxycytidine, 6% of the activity with ATP and deoxyadenosine

642251, 642248, 642254, 642253, 642256, 642268

2.7.6.1

best cofactor, Pcal_1127 exhibits slightly higher activity with dATP compared to ATP as diphosphate donor

can substitute for ATP

can substitute for ATP

775949

Activator in Enzyme-catalyzed Reactions (429 results)

highly activating

positive effector for CTP reduction

nearly as effective as ATP

both dATP and dGTP are co-activators for hydrolysis of dTTP, dATP might bind at the secondary allosteric site

activates

at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates

692974

4.6.1.2

poor allosteric activator

Inhibitor in Enzyme-catalyzed Reactions (798 results)

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1.1.1.205

18% inhibition at 0.5 mM

4% inhibition

strong feedback inhibition, tight binding at the active site in stoichiometric amount

661195

2.7.1.43

with ATP as substrate

more than 95% inhibition at 0.3 mM

inhibition at 1 mM

mechanism of Mn2+-dependent inhibition

IC50 above 0.5 mM

5 mM, 28°C, presence of MgCl2, 39% residual activity

693138

2.7.7.B16

synthesis of the short RNA chains is inhibited at all levels of dATP added, and the size of oligo(rA) chains formed and the amount of ATP incorporated are reduced

purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs

0.2 mM, 15% residual activity

competitive to ATP

the 8-oxo-dGTPase activity of wild type hMTH1 decreases to 79.6% of the control in the presence of 0.1 mM dATP

83% and 27% inhibition of RNA and DNA helicase activity at 2.5 mM, respectively

3D Structure of Enzyme-Ligand-Complex (PDB) (2767 results)

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (93 results)

0.9

pH 7.5, 30°C

0.16

50°C, pH 7.5, synthesis of full-length primer

0.833

0.022

at pH 8.0 and 37°C

0.37

in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C

0.15

in the presence of 5 mM Mn2+, at pH 8.0 and 37°C

757347

3.6.1.65

1.05

pH and temperature not specified in the publication

756613

3.6.1.67

4.59

pH 8.5, 37°C

728808

3.6.1.9

1.12

pH 8.0, 70°C

748152

5.6.2.3

14.8

pH 6.6, 25°C

656166

5.6.2.4

2 entries

K_M Value (146 results)

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1.17.4.1

0.003

positive effector of CDP reduction

4.2

pH 6.8, 30°C, reaction with sn-2,3-dihexanoylglycerol

0.138

1.2

pH 7.5, 30°C

641750

2.7.1.7

0.6

pH 7.5, 25°C

0.13

pH 7.2, 37°C

645193

2.7.6.1

0.233

pH 7.6, 37ºC, isoenzyme 3

642738

2.7.7.1

0.45

37°C

642798

2.7.7.102

0.032

50°C, pH 7.5, synthesis of full-length primer

720578

2.7.7.19

0.06

30°C, pH 8.5

0.027

pH 8, 30°C

0.35

pH 7, 25ºC

0.048

0.155

at pH 8.0 and 37°C

0.1143

in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C

0.298

in the presence of 5 mM Mn2+, at pH 8.0 and 37°C

757347

3.6.1.65

1.2

pH and temperature not specified in the publication

756613

3.6.1.67

0.79

pH 8.5, 37°C

0.44

0.25

pH 8.0, 30°C, in presence of ssDNA

694418

6.1.1.11

0.065

6.1.1.19

1.5

NTP-diphosphate exchange

2.3

12.5

13.3

758, 764

6.3.2.17

0.05

reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP

0.0013

K_i Value (20 results)

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1.1.98.6

0.012

substrate ATP, pH not specified in the publication, temperature not specified in the publication

0.7

0.041

pH 7.6, 37°C, recombinant deoxyguanosine kinase

640562

2.7.1.67

0.014

pH 7.4, 25°C, in presence of 3 mM spermidine

0.048

2.8

at pH 7.0, 30°C

1.39

0.001

0.6

0.6

pH 7.5, 25°C, inhibition of ATPase activity

656494

3.6.1.61

0.041

pH 7.0, 25°C

715466

5.6.2.4

0.291

inhibition of NTPase activity of NS3 protein by NTP derivatives

696161

5.6.2.6

2 entries

IC₅₀ Value (2 results)

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2.7.7.48

0.5

IC50 above 0.5 mM

643393

5.6.2.6

0.9

pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction

713144

References & Links

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Show Reference (533 entries)

Links to other databases for dATP

Ligand dATP

Basic Ligand Information

Related pathways

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (14 results)

In Vivo Product in Enzyme-catalyzed Reactions (23 results)

Substrate in Enzyme-catalyzed Reactions (674 results)

Product in Enzyme-catalyzed Reactions (37 results)

Enzyme Cofactor/Cosubstrate (32 results)

Activator in Enzyme-catalyzed Reactions (429 results)

Inhibitor in Enzyme-catalyzed Reactions (798 results)

3D Structure of Enzyme-Ligand-Complex (PDB) (2767 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (93 results)

KM Value (146 results)

Ki Value (20 results)

IC50 Value (2 results)

References & Links

Literature References (533)

Links to other databases for dATP

k_cat Value (Turnover Number) (93 results)

K_M Value (146 results)

K_i Value (20 results)

IC₅₀ Value (2 results)