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Ligand 4-hydroxy-3-methylbenzoate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.13.82

4-hydroxy-3-methylbenzoate + O2 + NADH + H+ = ?

744457

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Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.13.82

4-hydroxy-3-methylbenzoate + O2 + NADH + H+ = 4-hydroxy-3-(hydroxymethyl)benzoate + NAD+ + H2O + formaldehyde

744457

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1.14.13.82

4-hydroxy-3-methylbenzoate + O2 + NADH + H+ = ?

744457

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1.14.13.82

2 entries

6.3.1.15

ATP + 4-hydroxy-3-methylbenzoate + 3-amino-4,7-dihydroxy-8-methylcoumarin = AMP + diphosphate + 3-[(2E)-but-2-en-1-yl]-N-(2,7-dihydroxy-8-methyl-4-oxo-4H-chromen-3-yl)-4-hydroxybenzylamide

722616, 723619

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Product in Enzyme-catalyzed Reactions (4 results)

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Show Product (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.14.92

3-methylbenzoate + [reduced NADPH-hemoprotein reductase] + O2 = 3-methyl-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O

440261

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1.14.14.92

3-methylbenzoic acid + [reduced NADPH-hemoprotein reductase] + O2 = 4-hydroxy-3-methylbenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O

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1.2.1.96

4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O = 4-hydroxy-3-methylbenzoic acid + NADH + H+

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-

1.2.1.96

4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O = 4-hydroxy-3-methylbenzoic acid + NADPH + H+

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-

1.14.14.92

2 entries

1.2.1.96

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

6.3.1.15

0.36

-

pH 7.0, temperature not specified in the publication

723619

K_M Value (1 result)

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Show KM Value (1 entry)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

6.3.1.15

0.36

-

pH 7.0, temperature not specified in the publication

723619

References & Links

Literature References (4)

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Show Reference (4 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

440261

11594739

Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger

2001

Faber, B.W.; van Gorcom, R.F.; Duine, J.A.

Arch. Biochem. Biophys.

394

245-254

722616

10801869

Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891

2000

Steffensky, M.; Li, S.M.; Heide, L.

J. Biol. Chem.

275

21754-21760

723619

15218104

Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton

2004

Pi, N.; Meyers, C.L.; Pacholec, M.; Walsh, C.T.; Leary, J.A.

Proc. Natl. Acad. Sci. USA

101

10036-10041

744457

25008238

Expression and substrate range of Streptomyces vanillate demethylase

2014

Nishimura, M.; Nishimura, Y.; Abe, C.; Kohhata, M.

Biol. Pharm. Bull.

37

1564-1568

Links to other databases for 4-hydroxy-3-methylbenzoate

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Show Links (1 entry)

ChEBI

PubChem

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