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Ligand 2',5'-dinitrophenyl alpha-L-arabinofuranoside Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C1 1 H1 2 N2 O9
2',5'-dinitrophenyl alpha-L-arabinofuranoside
NBJCJDLOPXQBOQ-UKKRHICBSA-N
2,5-dinitrophenyl-alpha-L-arabinofuranoside
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (2 results)
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2',5'-dinitrophenyl alpha-L-arabinofuranoside + H2O = 2,5-dinitrophenol + alpha-L-arabinofuranose
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2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O = 2,5-dinitrophenol + L-arabinofuranose
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (4 results)
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0.69
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pH 6.5, mutant enzyme D299G
0.72
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pH 6.0, 40°C, mutant E175A
8.25
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pH 6.5, wild-type enzyme
190
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pH 6.0, 40°C, wild-type enzyme
KM Value (4 results)
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0.003
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pH 6.0, 40°C, mutant E175A
0.1
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pH 6.5, mutant enzyme D299G
0.28
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pH 6.5, wild-type enzyme
0.35
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pH 6.0, 40°C, wild-type enzyme
References & Links Literature References (2)
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Detailed kinetic analysis and identification of the nucleophile in alpha-L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase
2002
Shallom, D.; Belakhov, V.; Solomon, D.; Shoham, G.; Baasov, T.; Shoham, Y.
J. Biol. Chem.
277
43667-43673
Mutagenesis and mechanistic study of a glycoside hydrolase family 54 alpha-L-arabinofuranosidase from Trichoderma koningii
2007
Wan, C.F.; Chen, W.H.; Chen, C.T.; Chang, M.D.; Lo, L.C.; Li, Y.K.
Biochem. J.
401
551-558
Links to other databases for 2',5'-dinitrophenyl alpha-L-arabinofuranoside