Ligand Dithionite

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Basic Ligand Information

Molecular Structure
Picture of Dithionite (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H2O4S2
Dithionite
ROFVEXUMMXZLPA-UHFFFAOYSA-N
Synonyms:
hydrosulfite, S2O42-

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (24 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
CO + ATP + dithionite + ? = ethene + ethane + propane + ?
show the reaction diagram
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dithionite + H+ + N2 + ATP = ?
show the reaction diagram
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3,5-diiodo-L-tyrosine + dithionite = 3-iodo-L-tyrosine + ? + I-
show the reaction diagram
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protochlorophyllide + dithionite = chlorophyllide + SO2
show the reaction diagram
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a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 dithionite + 16 H+ = a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized dithionite
show the reaction diagram
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dithionite + amicyanin + H2O = ?
show the reaction diagram
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ferricytochrome c + dithionite = ferrocytochrome c + ?
show the reaction diagram
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nitrite + dithionite = NO + reduced dithionite
show the reaction diagram
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adenylyl sulfate + dithionite = AMP + sulfite + ?
show the reaction diagram
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thiosulfate + dithionite = ?
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
menaquinone-7 + reduced dithionite = menaquinone-7 with partially saturated prenyl side chain + dithionite
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (12 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
dark protochlorophyllide reductase activity is dependent on the reductant dithionite
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rapidly activates sQH-AmDH, activation process involves a reduction process
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presence of reducing agents is essential, and dithionite is most effective among reagents tested
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after activation with Fe2+, HS-, S-adenosyl-L-methionine and dithionite, activity increases almost 10fold
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reduction of the FAD cofactor with dithionite increases the quantum yield of repair
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a strong reductant is required for activity
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after activation with Fe2+, HS-, S-adenosyl-L-methionine and dithionite, activity increases almost 10fold
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catalytic activity of the as-purified enzyme requires pretreatment with dithionite to a final concentration of 10 mM to create reducing conditions
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activity absolutely depends on the addition of dithionite
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Inhibitor in Enzyme-catalyzed Reactions (37 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 75%, 0.25 mM inhibits by 92%
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10% inhibition at 1 mM, 95% inhibition at 10 mM
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reduction of FMN
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reactivation by oxidation
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reduction of RoxA results in a reversible inactivation of the enzyme
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1 mM, 15% inhibition
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34% inhibition at 5 mM
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with 5 mM dithionite, the half-life is 7 h at 2 °C, with about 90% of the original activity being lost after 24 h
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2 mM sodium dithionite: complete inhibition
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irreversible formation of inactive blue form of the enzyme
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1 mM, complete inhibition
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irreversible breakdown of the Fe-Fe interaction in the [2Fe-2S] cluster
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inhibits reverse methyl group transfer, when it is preincubated with methylated enzyme but not when it is preincubated with Co+-iron-sulfur protein
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inhibition at 40 mM
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in air, inactivation
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strong inhibition at 1 mM
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inactivation, oxidation with potassium hexacyanoferrate(III) results in up to 84% reactivation
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2fold decrease in enzyme activity due to altered oxidation state of the heme
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Metals and Ions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates
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3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

KM Value (1 result)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0527
-
in 100 mM HEPES-NaOH (pH 7.5), 2 mM ATP, 5 mM MgCl2

References & Links

Links to other databases for Dithionite

ChEBI
PubChem
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PubChem