Ligand folate

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Basic Ligand Information

Molecular Structure
Picture of folate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C19H19N7O6
folate
OVBPIULPVIDEAO-LBPRGKRZSA-N
Synonyms:
7,8-dihydropteroyl-L-Glu, dihydropteroylglutamic acid, dihydropteroylmonoglutamate, Folate analogs, folic acid, Folic acid analogues, Folic acid conjugates, Folic acid derivatives, folic acid[side 1], folic acid[side 2], monoglutamyl folate, oxidized tetrahydrofolate, pteroyl-Glu, pteroylglutamate, pteroylglutamates, pteroylglutamic acid, pteroylmonoglutamate, short-chain pteroylglutamates, substituted and reduced pteroylglutamate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
folate + NADPH + H+ = 7,8-dihydrofolate + tetrahydrofolate + NADP+
show the reaction diagram
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folate + H2O = pteroate + glutamate
show the reaction diagram
-
folic acid + H2O = pteroic acid + L-glutamate
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
polyglutamyl-folate + H2O = monoglutamyl folate + glutamate
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (63 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
dihydropteroylglutamic acid + NAD(P)H = tetrahydropteroylglutamate + NAD(P)+
show the reaction diagram
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folic acid + H2O = pteroic acid + glutamic acid
show the reaction diagram
-
folate + H2O = pteroate + glutamate
show the reaction diagram
-
folic acid + H2O = pteroic acid + L-glutamate
show the reaction diagram
-
ATP + pteroyl-Glu + Glu = ADP + phosphate + pteroylmonoglutamyl-Glu
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
2 L-arginine + 3 tetrahydrofolate + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 oxidized tetrahydrofolate + 4 H2O
show the reaction diagram
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-
N10-formylfolate + glycinamide ribonucleotide = folate + formylglycinamide ribonucleotide
show the reaction diagram
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ATP + 7,8-dihydropteroate + L-Glu = ADP + phosphate + 7,8-dihydropteroyl-L-Glu
show the reaction diagram
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-

Enzyme Cofactor/Cosubstrate (9 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
dependent on
-
folate binds at the enzyme's active site, and facilitate acetyl coenzyme A hydrolysis
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Activator in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates
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Inhibitor in Enzyme-catalyzed Reactions (309 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
uncompetitive inhibition of oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system
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inhibition of NADH-lipoamide oxidoreductase activity, no effect on diaphorase activity and transhydrogenase activity
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the native rat liver GNMT contains an acetylated N-terminal valine and is inhibited much more efficiently compared to the recombinant protein
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competitive
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1.2 mM, complete inhibition of exchange between free guanine with guanine
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0.1 mM: not, 30% inhibition at 1.0 mM
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substrate inhibition, competitive
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extra cellular enzyme complex inhibition
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inhibitor when 2',3'-CMP is substrate not when RNA is substrate
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IC50 ketonase reaction 62.7 microM, pH 6.5, room temperatur, IC50 enolase reaction 80.2 microM, pH 6.2, room temperatur
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3D Structure of Enzyme-Ligand-Complex (PDB) (1321 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (4 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.65
-
-

KM Value (26 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (11 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0076
-
-
0.1
-
-
0.0337
-
-
0.011
-
pH and temperature not specified in the publication
0.015
-
-
0.058
-
at 37°C in 100 mM MES, pH 6.0

IC50 Value (5 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.0525
-
pH and temperature not specified in the publication
0.052
-
pH 7.4, 37°C, versus 4-aminobenzoylglutamate

References & Links

Links to other databases for folate

ChEBI
PubChem
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PubChem