Ligand ferricyanide

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Basic Ligand Information

Molecular Structure
Picture of ferricyanide (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C6FeN6
ferricyanide
CRWNKXHVYMVLCY-UHFFFAOYSA-N
Synonyms:
(Fe(CN)6)3-, Fe(CN)3-, Fe(CN)3[side 2], Fe(CN)6 3-, Fe(CN)63-, ferricyanide[side 2], hexacyanoferrate(III), hexacyanoferrate (III), [Fe(CN)6]3-, [Fe(CN6)]3-

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
(S)-mandelate + 2 ferricyanide = benzoylformic acid + 2 ferrocyanide + 2 H+
show the reaction diagram
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taurine + H2O + ferricyanide = 2-sulfoacetaldehyde + NH3 + ferrocyanide
show the reaction diagram
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thiocyanate + 2 ferricyanide + H2O = cyanate + sulfur + 2 ferrocyanide + 2 H+
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (274 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
2 ferricyanide + NADPH = 2 ferrocyanide + NADP+ + H+
show the reaction diagram
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L-lactate + ferricyanide = pyruvate + ferrocyanide + H+
show the reaction diagram
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D-lactate + ferricyanide = pyruvate + ferrocyanide
show the reaction diagram
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D-glucose + ferricyanide = 2-dehydro-D-glucose + ferrocyanide
show the reaction diagram
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polyvinyl alcohol + ferricyanide = polyvinylketone + ferrocyanide
show the reaction diagram
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D-galactopyranose + ferricyanide = D-galacto-hexodialdose + ferrocyanide
show the reaction diagram
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D-lactate + ferricyanide = pyruvate + ferrocyanide
show the reaction diagram
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D-glucose + ferricyanide = D-glucono-1,5-lactone + ferrocyanide
show the reaction diagram
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sn-glycerol 3-phosphate + ferricyanide = glycerone phosphate + ferrocyanide
show the reaction diagram
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(R)-Pantolactone + (Fe(CN)6)3- = 2-Dehydropantonolactone + Fe(CN)64-
show the reaction diagram
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2-dehydro-D-gluconate + ferricyanide = 2,5-didehydro-D-gluconate + ferrocyanide
show the reaction diagram
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(R)-lactate + ferricyanide = pyruvate + ferrocyanide
show the reaction diagram
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H2O + ferricyanide + hv = O2 + ferrocyanide
show the reaction diagram
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ferricyanide + H2O2 = ferrocyanide + H2O
show the reaction diagram
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3-indolemethanol + ferricyanide = ?
show the reaction diagram
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4-hydroxybenzoate + NADPH + ferricyanide = protocatechuate + NADP+ + ferrocyanide
show the reaction diagram
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[methionine synthase]-cob(II)alamin + S-adenosyl-L-methionine + ferricyanide = [methionine synthase]methylcob(I)alamin + S-adenosylhomocysteine + ?
show the reaction diagram
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2 ferricyanide + NADPH = 2 ferrocyanide + NADP+ + H+
show the reaction diagram
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formate + ferricyanide = CO2 + ferrocyanide + H+
show the reaction diagram
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NADH + H+ + Fe(CN)63- = NAD+ + Fe(CN)62-
show the reaction diagram
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2 ferricyanide + NADH = 2 ferrocyanide + NAD+ + H+
show the reaction diagram
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pyruvate + CoA + ferricyanide = acetyl-CoA + CO2 + ferrocyanide
show the reaction diagram
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pyruvate + phosphate + ferricyanide + H2O = acetyl phosphate + ferrocyanide + H2O2
show the reaction diagram
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benzaldehyde + 2 ferricyanide + H2O = benzoate + 2 ferrocyanide + 2 H+
show the reaction diagram
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ferricyanide + O2 = ? + H2O
show the reaction diagram
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succinate + ferricyanide = fumarate + ferrocyanide
show the reaction diagram
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succinate + ferricyanide = fumarate + ferrocyanide
show the reaction diagram
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ferricyanide + NADPH + H+ = ferrocyanide + NADP+
show the reaction diagram
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taurine + H2O + ferricyanide = 2-sulfoacetaldehyde + NH3 + ferrocyanide
show the reaction diagram
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2 ferricyanide + NADH = 2 ferrocyanide + NAD+ + H+
show the reaction diagram
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L-proline + ferricyanide + H2O = (S)-1-pyrroline-5-carboxylate + ferrocyanide
show the reaction diagram
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N6-(2-isopentenyl)adenine 9-beta-D-riboside + ferricyanide + H2O = adenine 9-beta-D-riboside + 3-methylbut-2-enal + ferrocyanide
show the reaction diagram
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L-proline + ferricyanide = DELTA1-pyrroline-5-carboxylate + ferrocyanide
show the reaction diagram
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Fe(CN)6 3- + NADPH = Fe(CN)6 4- + NADP+
show the reaction diagram
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2 ferricyanide + NADH = 2 ferrocyanide + NAD+ + H+
show the reaction diagram
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2 ferricyanide + NADH = 2 ferrocyanide + NAD+ + H+
show the reaction diagram
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2 ferricyanide + NADPH = 2 ferrocyanide + NADP+ + H+
show the reaction diagram
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2 ferricyanide + NADPH = 2 ferrocyanide + NADP+ + H+
show the reaction diagram
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2 ferricyanide + NADH = 2 ferrocyanide + NAD+ + H+
show the reaction diagram
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2 ferricyanide + NADH = 2 ferrocyanide + NAD+ + H+
show the reaction diagram
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ferricyanide + NADPH + H+ = ferrocyanide + NADP+
show the reaction diagram
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thiocyanate + 2 ferricyanide + H2O = cyanate + sulfur + 2 ferrocyanide + 2 H+
show the reaction diagram
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sulfite + ferricyanide = sulfate + ferrocyanide
show the reaction diagram
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2 thiosulfate + ferricyanide = tetrathionate + ferrocyanide
show the reaction diagram
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ubiquinol + 2 ferricyanide = ubiquinone + 2 ferrocyanide + 2 H+
show the reaction diagram
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2 ferricyanide + NADH + n Na+/in = 2 ferrocyanide + NAD+ + H+ + n Na+/out
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (37 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
allyl alcohol + ferricyanide = acrolein + ferricyanide
show the reaction diagram
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ferrocyanide + H2O2 = ferricyanide + H2O
show the reaction diagram
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ferrocyanide + H+ + H2O2 = ferricyanide + H2O
show the reaction diagram
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ferrocyanide + H2O2 = ferricyanide + OH-
show the reaction diagram
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2 ferrocyanide + NADP+ + H+ = 2 ferricyanide + NADPH
show the reaction diagram
-
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Fe(CN)62- + NAD+ = Fe(CN)63- + NADH + H+
show the reaction diagram
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succinate + ferrocyanide = fumarate + ferricyanide
show the reaction diagram
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NADH + Fe(CN)63- = NAD+ + Fe(CN)63-
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (5 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activation, direct reduction by reductaseNAP in the presence of NADH in vitro
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in ascorbate-loaded vesicle membranes can supply electron equivalents to support extravesicular dopamine beta-hydroxylase activity without the addition of any mediator, this activity is enhanced significantly by the addition of ferricyanide
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1 mM, catalytic activity 102-119%
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Inhibitor in Enzyme-catalyzed Reactions (25 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
inhibition at 0.001 mM, activation at 1 mM
-
0.82 mM, 50% inhibition of brominating activity
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2.5 mM, 80% decrease in activity within 10 min
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enzyme a, b, c and e
-
at concentrations above 3 mM
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inhibition of the recombinant FAD domain
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addition of 0.2 mM ferricyanide causes a rapid oxidation of the enzyme
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0.05 mM, complete inhibition
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2 mM, 17% inhibition
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partial, 28% inhibition
-
complete loss of activity
-
inhibits the interaction of the oxidized enzyme with NADH
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Metals and Ions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulates
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (124 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.3
-
substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
230
-
apparent turnover number
550
-
pH 7.5, 25°C
8.24
-
variant I22/S175
18.2
-
cosubstrate xanthine
60.2
-
pH 8.5, 30°C
33.7
-
pH 7.2, 30°C
26.8
-
pH 7.5, 30°C
157
-
-
618
-
-
111
-
pH 7.8, temperature not specified in the publication
17000
-
-

KM Value (168 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.23
-
-
3.43
-
substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.69
-
pH 8.8, 25°C
0.55
-
-
0.025
-
isolated subunit I
0.33
-
at 30°C with 25 mM glucose as susbtrate
2.5
-
-
3.87
-
at pH 6.5 and 30°C
0.1
-
-
0.67
-
-
0.055
-
-
0.16
-
pH 7.2, 30°C
0.0236
-
pH 7.5, 30°C
0.03
-
as electron acceptor under aerobic conditions
0.3
-
-
0.14
-
of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme
0.1193
-
at pH 7.4 and 25°C
0.0009
-
pH 7.8, temperature not specified in the publication
0.56
-
-
0.698
-
-

Ki Value (3 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
23
-
inhibition of the recombinant FAD domain

References & Links

Links to other databases for ferricyanide

ChEBI
PubChem
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PubChem