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Ligand 1-deoxy-D-xylulose 5-phosphate

Basic Ligand Information

Molecular Structure

Related pathways

Pathway Source

Pathways

BRENDA

isoprenoid biosynthesis, vitamin B1 metabolism, vitamin B6 metabolism

KEGG

Biosynthesis of alkaloids derived from terpenoid and polyketide, Biosynthesis of cofactors, Biosynthesis of plant hormones, Biosynthesis of plant secondary metabolites, Biosynthesis of secondary metabolites, Biosynthesis of terpenoids and steroids, Cysteine and methionine metabolism, Terpenoid backbone biosynthesis, Thiamine metabolism more

MetaCyc

methylerythritol phosphate pathway I, methylerythritol phosphate pathway II, pyridoxal 5'-phosphate biosynthesis I, S-methyl-5-thio-alpha-D-ribose 1-phosphate degradation II, thiazole component of thiamine diphosphate biosynthesis I, thiazole component of thiamine diphosphate biosynthesis II more

Show all BRENDA pathways known for 1-deoxy-D-xylulose 5-phosphate

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Natural Substrate (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.267

1-deoxy-D-xylulose 5-phosphate + NADPH + H+ = 2-C-methyl-D-erythritol 4-phosphate + NADP+

286421, 286422, 286423, 286427, 286428, 286425, 286429, 286424, 286420, 286426, 671590, 676736, 673073, 673562, 672538, 672396, 676660, 671075, 672040, 674260, 675038, 675577, 675580, 676579, 725663, 720429, 723812, 724374, 724346, 724543, 725564, 760333, 762667, 762747, 763591, 762782, 762794, 763279, 763181, 762525, 748373, 763547, 763576, 762871, 763000, 763120, 763482, 763607

1Q0Q, 3D-view

2.6.99.2

1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + H2O

207962, 664163, 207963, 666951, 666014, 664004

-

2.8.1.10

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O

739422, 737721

5Z9Y, 3D-view

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

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Show Natural Product (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.2.1.7

pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2

-

-

2.7.1.17

ATP + 1-deoxy-D-xylulose = ADP + 1-deoxy-D-xylulose 5-phosphate

645057

-

2.8.4.6

S-methyl-1-thio-D-xylulose 5-phosphate + glutathione = 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione

-

-

Substrate in Enzyme-catalyzed Reactions (9 results)

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Show Substrate (9 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.267

1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ = 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+

673562, 676660, 684434, 699265

1Q0Q, 3D-view

1.1.1.267

1-deoxy-D-xylulose 5-phosphate + NADPH + H+ = 2-C-methyl-D-erythritol 4-phosphate + NADP+

286421, 286422, 286423, 286427, 286428, 286425, 286429, 286424, 286420, 286426, 671590, 676736, 673073, 673562, 672538, 672396, 676660, 671075, 672040, 674260, 675038, 675577, 675580, 676579, 698490, 691382, 697249, 697335, 712892, 713353, 725663, 720429, 723812, 724374, 724346, 724543, 725564, 740062, 741292, 760333, 762667, 762747, 763591, 762782, 762794, 763279, 763181, 762525, 748373, 763547, 763576, 762871, 763000, 763120, 763482, 763607

1Q0Q, 3D-view

1.1.1.267

1-deoxy-D-xylulose 5-phosphate + NADPH = ? + NADP+

698490

1Q0Q, 3D-view

1.1.1.267

1-deoxy-D-xylulose-5-phosphate + NADH = methyl-D-erythritol-4-phosphate + NAD+

654748

1Q0Q, 3D-view

1.1.1.267

1-deoxy-D-xylulose-5-phosphate + NADPH = methyl-D-erythritol-4-phosphate + NADP+

656890, 654877, 655439, 654748

1Q0Q, 3D-view

1.1.1.267

3-[2H]-D-1-deoxyxylulose-5-phosphate + NAD(P)H = ? + NAD(P)+

684434

1Q0Q, 3D-view

1.1.1.267

4-[2H]-D-1-deoxyxylulose-5-phosphate + NAD(P)H = ? + NAD(P)+

684434

1Q0Q, 3D-view

1.1.1.267

7 entries

2.6.99.2

1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + H2O

207962, 664163, 207963, 666951, 666014, 664004, 663470

-

2.8.1.10

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O

707466, 708874, 715306, 739422, 737721

5Z9Y, 3D-view

Product in Enzyme-catalyzed Reactions (12 results)

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Show Product (12 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.267

methyl-D-erythritol-4-phosphate + NADP+ = 1-deoxy-D-xylulose-5-phosphate + NADPH

-

1Q0Q, 3D-view

2.2.1.7

pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2

-

-

2.2.1.7

pyruvate + DL-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2

-

-

2.2.1.7

pyruvate + glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2

-

-

2.7.1.17

ATP + 1-deoxy-D-xylulose = ADP + 1-deoxy-D-xylulose 5-phosphate

645057, -

-

2.7.1.17

CTP + 1-deoxy-D-xylulose = CDP + 1-deoxy-D-xylulose 5-phosphate

645057

-

2.7.1.17

GTP + 1-deoxy-D-xylulose = GDP + 1-deoxy-D-xylulose 5-phosphate

645057

-

2.7.1.17

ITP + 1-deoxy-D-xylulose = IDP + 1-deoxy-D-xylulose 5-phosphate

645057

-

2.7.1.17

UTP + 1-deoxy-D-xylulose = UDP + 1-deoxy-D-xylulose 5-phosphate

645057

-

2.8.4.6

S-methyl-1-thio-D-xylulose 5-phosphate + dihydrolipoate = 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dihydrolipoate

-

-

2.8.4.6

S-methyl-1-thio-D-xylulose 5-phosphate + glutathione = 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione

-

-

2.8.4.6

S-methyl-1-thio-D-xylulose 5-phosphate + L-dithiothreitol = 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dithiothreitol

-

-

2.2.1.7

3 entries

2.7.1.17

5 entries

2.8.4.6

3 entries

Inhibitor in Enzyme-catalyzed Reactions (2 results)

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Show Inhibitors (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.267

substrate inhibition

674260

1Q0Q, 3D-view

2.2.1.7

competitive inhibition with respect to pyruvate, non-competitive inhibition with respect to D-glyceraldehyde 3-phosphate

756018

-

3D Structure of Enzyme-Ligand-Complex (PDB) (3 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (3 entries)

EC NUMBER

ENZYME 3D STRUCTURE

1.1.1.267

1Q0Q, 3D-view

2.7.1.12

3LL3, 3D-view

2.8.1.10

5Z9Y, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (19 results)

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Show Turnover Number (19 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.267

0.16

-

pH 7.8, 37°C, recombinant mutant W204A

671590

1.1.1.267

0.26

-

pH 7.8, 37°C, recombinant mutant W204L

671590

1.1.1.267

0.54

-

pH 7.8, 37°C, recombinant mutant W204V

671590

1.1.1.267

1.59

-

mutant W203F, pH 7.5, 25°C

740062

1.1.1.267

1.7

-

pH 7.8, 37°C

741292

1.1.1.267

2

-

pH 7.8, 22°C

713353

1.1.1.267

3.54

-

mutant W203Y, pH 7.5, 25°C

740062

1.1.1.267

4.2

-

wild-type, pH 7.5, 25°C

740062

1.1.1.267

4.4

-

pH 8.0, 37°C, recombinant pseudomature enzyme

673562

1.1.1.267

5.25

-

pH 7.5, 25°C, recombinant enzyme

724374, 724346

1.1.1.267

5.3

-

-

671075

1.1.1.267

10

-

pH 7.8, 37°C, recombinant mutant W204F

671590

1.1.1.267

13.2

-

-

698490

1.1.1.267

13.7

-

-

698490

1.1.1.267

16.1

-

-

698490

1.1.1.267

17

-

pH 7.8, 37°C, recombinant wild-type enzyme

671590

1.1.1.267

29

-

pH 7.6, 37°C, recombinant enzyme

672040, 675577

1.1.1.267

33

-

pH 7.6, 37°C, recombinant His-tagged enzyme

675580

1.1.1.267

38

-

pH 7.6, 37°C, recombinant enzyme

675577

1.1.1.267

19 entries

K_M Value (40 results)

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Show KM Value (40 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.267

0.0031

-

-

684434

1.1.1.267

0.004

-

cosubstrate NADPH, presence of Co2+, pH 7.5, 25°C

654748

1.1.1.267

0.00423

-

-

684434

1.1.1.267

0.00537

-

-

684434

1.1.1.267

0.0255

-

pH 7.6, 30°C, recombinant enzyme

724543

1.1.1.267

0.04

-

pH 7.5, 50°C

712892

1.1.1.267

0.042

-

cosubstrate NADPH, presence of Mg2+, pH 7.5, 25°C

654748

1.1.1.267

0.045

-

pH 7.6, 37°C, recombinant enzyme

672040

1.1.1.267

0.0471

-

pH 7.9, 30°C, recombinant His-tagged enzyme

674260

1.1.1.267

0.05

-

pH 7.6, 37°C, recombinant enzyme

675577

1.1.1.267

0.05

-

cosubstrate NADH, presence of Co2+, pH 7.5, 25°C

654748

1.1.1.267

0.06

-

with 1 mM CoCl2

286428

1.1.1.267

0.061

-

-

656890

1.1.1.267

0.063

-

pH 7.5, 37°C, presence of 1 mM Co2+

654877

1.1.1.267

0.073

-

pH 7.7, presence of 1 mM MnCl2

655439

1.1.1.267

0.081

-

pH 7.6, 37°C, recombinant His-tagged enzyme

675580

1.1.1.267

0.097

-

pH 7.7, presence of 3 mM MgCl2

655439

1.1.1.267

0.099

-

with 1 mM MgCl2

286428

1.1.1.267

0.1

-

cosubstrate NADPH, presence of Mn2+, pH 7.5, 25°C

654748

1.1.1.267

0.1037

-

pH 7.8, 22°C

713353

1.1.1.267

0.11

-

pH 7.5, 85°C

712892

1.1.1.267

0.115

-

pH 7.5, 25°C, recombinant enzyme

724374, 724346

1.1.1.267

0.132

-

pH 8.0, 37°C, recombinant pseudomature enzyme

673562

1.1.1.267

0.134

-

pH 7.5, 37°C, presence of 1 mM Mg2+

654877

1.1.1.267

0.137

-

wild-type, pH 7.5, 25°C

740062

1.1.1.267

0.147

-

pH 8.0, 37°C, recombinant enzyme

676660

1.1.1.267

0.17

-

pH 7.8, 37°C, recombinant wild-type enzyme

671590

1.1.1.267

0.195

-

pH 7.5, 37°C, presence of 1 mM Mn2+

654877

1.1.1.267

0.21

-

cosubstrate NADH, presence of Mg2+, pH 7.5, 25°C

654748

1.1.1.267

0.222

-

pH 7.8, 37°C

741292

1.1.1.267

0.24

-

cosubstrate NADH, presence of Mn2+, pH 7.5, 25°C

654748

1.1.1.267

0.25

-

with 1 mM MnCl2

286428

1.1.1.267

0.3

-

-

286424

1.1.1.267

0.34

-

-

671075

1.1.1.267

0.39

-

mutant W203Y, pH 7.5, 25°C

740062

1.1.1.267

0.42

-

pH 7.8, 37°C, recombinant mutant W204F

671590

1.1.1.267

1.015

-

mutant W203F, pH 7.5, 25°C

740062

1.1.1.267

2.9

-

pH 7.8, 37°C, recombinant mutant W204L

671590

1.1.1.267

5

-

pH 7.8, 37°C, recombinant mutant W204V

671590

1.1.1.267

12

-

pH 7.8, 37°C, recombinant mutant W204A

671590

1.1.1.267

40 entries

K_i Value (3 results)

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Show KI Value (3 entries)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.267

1.1

-

pH 7.9, 30°C, recombinant His-tagged enzyme

674260

2.2.1.7

0.13

-

inhibition with respect to pyruvate, at pH 8.0 and 37°C

756018

2.2.1.7

0.83

-

inhibition with respect to D-glyceraldehyde 3-phosphate, at pH 8.0 and 37°C

756018

2.2.1.7

2 entries

References & Links

Literature References (87)

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Show Reference (87 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

207962

10225425

Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein

1999

Laber, B.; Maurer, W.; Scharf, S.; Stepusin, K.; Schmidt, F.S.

FEBS Lett.

449

45-48

207963

-

Biosynthesis of vitamin B6: enzymatic conversion of 1-deoxy-D-xylulose-5-phosphate to pyridoxol phosphate

1999

Cane, D.E.; Du, S.; Robinson, J.K.; Hsiung, Y.; Spenser, I.D.

J. Am. Chem. Soc.

121

7722-7723

286420

11427737

Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase

2001

Mahmoud, S.S.; Croteau, R.B.

Proc. Natl. Acad. Sci. USA

98

8915-8920

286421

10631325

Biosynthesis of terpenoids: 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Escherichia coli is a class B dehydrogenase

2000

Radykewicz, T.; Rohdich, F.; Wungsintaweekul, J.; Herz, S.; Kis, K.; Eisenreich, W.; Bacher, A.; Zenk, M.H.; Arigoni, D.

FEBS Lett.

465

157-160

286422

11872159

Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: Implications of a flexible loop movement upon substrate binding

2002

Yajima, S.; Nonaka, T.; Kuzuyama, T.; Seto, H.; Ohsawa, K.

J. Biochem.

131

313-317

286423

11741911

Crystal structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase, a crucial enzyme in the non-mevalonate pathway of isoprenoid biosynthesis

2002

Reuter, K.; Sanderbrand, S.; Jomaa, H.; Wiesner, J.; Steinbrecher, I.; Beck, E.; Hintz, M.; Klebe, G.; Stubbs, M.T.

J. Biol. Chem.

277

5378-5384

286424

11004410

Isolation of the dxr gene of Zymomonas mobilis and characterization of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase

2000

Grolle, S.; Bringer-Meyer, S.; Sahm, H.

FEMS Microbiol. Lett.

191

131-137

286425

11034300

Tools for discovery of inhibitors of the 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: an approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosa

2000

Altincicek, B.; Hintz, M.; Sanderbrand, S.; Wiesner, J.; Beck, E.; Jomaa, H.

FEMS Microbiol. Lett.

190

329-333

286426

10222052

Isoprenoid biosynthesis via a mevalonate-independent pathway in plants: Cloning and heterologous expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from peppermint

1999

Lange, B.M.; Croteau, R.

Arch. Biochem. Biophys.

365

170-174

286427

9707569

A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis

1998

Takahashi, S.; Kuzuyama, T.; Watanabe, H.; Seto, H.

Proc. Natl. Acad. Sci. USA

95

9879-9884

286428

10787409

Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues

2000

Kuzuyama, T.; Takahashi, S.; Takagi, M.; Seto, H.

J. Biol. Chem.

275

19928-19932

286429

11162369

Plasmodium falciparum: Detection of the deoxyxylulose 5-phosphate reductoisomerase activity

2000

Wiesner, J.; Hintz, M.; Altincicek, B.; Sanderbrand, S.; Weidemeyer, C.; Beck, E.; Jomaa, H.

Exp. Parasitol.

96

182-186

645057

11168365

Phosphorylation of 1-deoxy-D-xylulose by D-xylulokinase of Escherichia coli

2001

Wungsintaweekul, J.; Herz, S.; Hecht, S.; Eisenreich, W.; Feicht, R.; Rohdich, F.; Bacher, A.; Zenk, M.H.

Eur. J. Biochem.

268

310-316

654748

15065882

Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductase

2004

Argyrou, A.; Blanchard, J.S.

Biochemistry

43

4375-4384

654877

14580998

Characterization of native and histidine-tagged deoxyxylulose 5-phosphate reductoisomerase from the cyanobacterium Synechocystis sp. PCC6803

2003

Yin, X.; Proteau, P.J.

Biochim. Biophys. Acta

1652

75-81

655439

12230556

Isoprenoid biosynthesis via the methylerythritol phosphate pathway. Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase

2002

Hoeffler, J.F.; Tritsch, D.; Grosdemange-Billiard, C.; Rohmer, M.

Eur. J. Biochem.

269

4446-4457

656890

15387564

Study of 1-deoxy-D-xylulose-5-phosphate reductoisomerase: synthesis and evaluation of fluorinated substrate analogues

2004

Wong, A.; Munos, J.W.; Devasthali, V.; Johnson, K.A.; Liu, H.W.

Org. Lett.

6

3625-3628

663470

10944349

Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme

2000

Garrido Franco, M.; Huber, R.; Schmidt, F.S.; Laber, B.; Clausen, T.

Acta Crystallogr. Sect. D

56

1045-1048

664004

12269807

Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate

2002

Yeh, J.I.; Du, S.; Pohl, E.; Cane, D.E.

Biochemistry

41

11649-11657

664163

12686115

Pyridoxine 5'-phosphate synthase: de novo synthesis of vitamin B6 and beyond

2003

Garrido-Franco, M.

Biochim. Biophys. Acta

1647

92-97

666014

12206776

Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis

2002

Garrido-Franco, M.; Laber, B.; Huber, R.; Clausen, T.

J. Mol. Biol.

321

601-612

666951

11286891

Structural basis for the function of pyridoxine 5'-phosphate synthase

2001

Franco, M.G.; Laber, B.; Huber, R.; Clausen, T.

Structure

9

245-253

671075

16790937

The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target

2006

Henriksson, L.M.; Bjoerkelid, C.; Mowbray, S.L.; Unge, T.

Acta Crystallogr. Sect. D

D62

807-813

671590

16289362

Mutation in the flexible loop of 1-deoxy-D-xylulose 5-phosphate reductoisomerase broadens substrate utilization

2005

Fernandes, R.P.; Phaosiri, C.; Proteau, P.J.

Arch. Biochem. Biophys.

444

159-164

672040

15938625

Mechanistic studies with 2-C-methyl-D-erythritol 4-phosphate synthase from Escherichia coli

2005

Fox, D.T.; Poulter, C.D.

Biochemistry

44

8360-8368

672396

16219495

Kinetic characterization of Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomerase mutants

2006

Fernandes, R.P.; Proteau, P.J.

Biochim. Biophys. Acta

1764

223-229

672538

16310360

Evaluation of fosmidomycin analogs as inhibitors of the Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomerase

2006

Woo, Y.H.; Fernandes, R.P.; Proteau, P.J.

Bioorg. Med. Chem.

14

2375-2385

673073

16116659

A fragment-based approach to understanding inhibition of 1-deoxy-D-xylulose-5-phosphate reductoisomerase

2005

Merckle, L.; de Andres-Gomez, A.; Dick, B.; Cox, R.J.; Godfrey, C.R.

Chembiochem

6

1866-1874

673562

16972937

Isoprenoid biosynthesis in plants - 2C-methyl-D-erythritol-4-phosphate synthase (IspC protein) of Arabidopsis thaliana

2006

Rohdich, F.; Lauw, S.; Kaiser, J.; Feicht, R.; Koehler, P.; Bacher, A.; Eisenreich, W.

FEBS J.

273

4446-4458

674260

16321944

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Identification of class 2 1-deoxy-D-xylulose 5-phosphate synthase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase genes from Ginkgo biloba and their transcription in embryo culture with respect to ginkgolide biosynthesis

2006

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2007

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Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis

2008

Sando, T.; Takeno, S.; Watanabe, N.; Okumoto, H.; Kuzuyama, T.; Yamashita, A.; Hattori, M.; Ogasawara, N.; Fukusaki, E.; Kobayashi, A.

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2008

Giessmann, D.; Heidler, P.; Haemers, T.; Van Calenbergh, S.; Reichenberg, A.; Jomaa, H.; Weidemeyer, C.; Sanderbrand, S.; Wiesner, J.; Link, A.

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2008

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2010

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Kinetic characterization and phosphoregulation of the Francisella tularensis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (MEP synthase)

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2011

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Antimalarial and structural studies of pyridine-containing inhibitors of 1-deoxyxylulose-5-phosphate reductoisomerase

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Pre-steady-state kinetic analysis of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Mycobacterium tuberculosis reveals partially rate-limiting product release by parallel pathways

2012

Liu, J.; Murkin, A.S.

Biochemistry

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2013

Kholodar, S.A.; Murkin, A.S.

Biochemistry

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Expression, characterization and inhibition of Toxoplasma gondii 1-deoxy-D-xylulose-5-phosphate reductoisomerase

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Design, synthesis, and X-ray crystallographic studies of alpha-aryl substituted fosmidomycin analogues as inhibitors of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase

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Andaloussi, M.; Henriksson, L.M.; Wieckowska, A.; Lindh, M.; Bjoerkelid, C.; Larsson, A.M.; Suresh, S.; Iyer, H.; Srinivasa, B.R.; Bergfors, T.; Unge, T.; Mowbray, S.L.; Larhed, M.; Jones, T.A.; Karlen, A.

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Structural Basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases

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Zhang, X.; Eser, B.E.; Chanani, P.K.; Begley, T.P.; Ealick, S.E.

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The thiG gene is required for full virulence of Xanthomonas oryzae pv. oryzae by preventing cell aggregation

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Alteration of the flexible loop in 1-deoxy-D-xylulose-5-phosphate reductoisomerase boosts enthalpy-driven inhibition by fosmidomycin

2014

Kholodar, S.A.; Tombline, G.; Liu, J.; Tan, Z.; Allen, C.L.; Gulick, A.M.; Murkin, A.S.

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Kinetic characterization and allosteric inhibition of the Yersinia pestis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (MEP synthase)

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Haymond, A.; Johny, C.; Dowdy, T.; Schweibenz, B.; Villarroel, K.; Young, R.; Mantooth, C.J.; Patel, T.; Bases, J.; San Jose, G.; Jackson, E.R.; Dowd, C.S.; Couch, R.D.

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Expression of genes 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), sabinene synthase (Tctps1) and alpha-terpineol synthase (Tctps5) and its relation to thymol and carvacrol biosynthesis in Thymus vulgaris under water deficit stress

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Mechanistic studies of 1-deoxy-D-xylulose-5-phosphate synthase from Deinococcus radiodurans

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Handa, S.; Dempsey, D.R.; Ramamoorthy, D.; Cook, N.; Guida, W.C.; Spradling, T.J.; White, J.K.; Woodcock, H.L.; Merkler, D.J.

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In silico characterization and differential expression analysis of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) of Centella asiatica

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3 Biotech

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3 Biotech

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Antimicrobial mechanism of epigallocatechin gallate and gallocatechin gallate they target 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway

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Hui, X.; Hua, S.H.; Wu, Q.Q.; Li, H.; Gao, W.Y.

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Structural characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Vibrio vulnificus

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Ussin, N.K.; Bagnell, A.M.; Offermann, L.R.; Abdulsalam, R.; Perdue, M.L.; Magee, P.; Chruszcz, M.

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Structural characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Vibrio vulnificus

2018

Ussin, N.K.; Bagnell, A.M.; Offermann, L.R.; Abdulsalam, R.; Perdue, M.L.; Magee, P.; Chruszcz, M.

Biochim. Biophys. Acta

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Cloning, characterization, and expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene from Dioscorea zingiberensis

2019

Cheng, Z.; Wang, R.; Shen, J.; Yang, Z.; Chen, Y.

Biol. Plant.

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Synthesis and biological evaluation of phosphate isosters of fosmidomycin and analogs as inhibitors of Escherichia coli and Mycobacterium smegmatis 1-deoxyxylulose 5-phosphate reductoisomerases

2017

Munier, M.; Tritsch, D.; Krebs, F.; Esque, J.; Hemmerlin, A.; Rohmer, M.; Stote, R.; Grosdemange-Billiard, C.

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The organ-specific differential roles of rice DXS and DXR, the first two enzymes of the MEP pathway, in carotenoid metabolism in Oryza sativa leaves and seeds

2020

You, M.K.; Lee, Y.J.; Kim, J.K.; Baek, S.A.; Jeon, Y.A.; Lim, S.H.; Ha, S.H.

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The organ-specific differential roles of rice DXS and DXR, the first two enzymes of the MEP pathway, in carotenoid metabolism in Oryza sativa leaves and seeds

2020

You, M.K.; Lee, Y.J.; Kim, J.K.; Baek, S.A.; Jeon, Y.A.; Lim, S.H.; Ha, S.H.

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Non-hydroxamate inhibitors of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) a critical review and future perspective

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Kesharwani, S.; Sundriyal, S.

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Molecular cloning and functional analysis of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Santalum album

2021

Zhang, Y.; Yan, H.; Li, Y.; Xiong, Y.; Niu, M.; Zhang, X.; Teixeira da Silva, J.A.; Ma, G.

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Molecular cloning and functional analysis of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Santalum album

2021

Zhang, Y.; Yan, H.; Li, Y.; Xiong, Y.; Niu, M.; Zhang, X.; Teixeira da Silva, J.A.; Ma, G.

Genes (Basel)

12

626

762871

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763000

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763120

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763181

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Expression analysis of gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and cardenolide and digitoxin production in digitalis purpurea L. using polyamines and methyl jasmonate as elicitors

2020

Rad, M.; Abdossi, V.; Moradi, P.; Rakhshandehroo, F.; Mehrafarin, A.

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A paradigm towards the antimalarial quest in silico identification and biological evaluation of novel inhibitors targeting 1-deoxy-D-xylulose-5-phosphate reductoisomerase

2020

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J. Biomol. Struct. Dyn.

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Inhibitory activity of plant essential oils against E. coli 1-deoxy-D-xylulose-5-phosphate reductoisomerase

2019

Yan, G.; Zhu, B.R.; Tian, F.L.; Hui, X.; Li, H.; Li, Y.M.; Gao, W.Y.

Molecules

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Inhibitory activity of plant essential oils against E. coli 1-deoxy-D-xylulose-5-phosphate reductoisomerase

2019

Yan, G.; Zhu, B.R.; Tian, F.L.; Hui, X.; Li, H.; Li, Y.M.; Gao, W.Y.

Molecules

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In silico identification of promiscuous scaffolds as potential inhibitors of 1-deoxy-D-xylulose 5-phosphate reductoisomerase for treatment of falciparum malaria

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Wadood, A.; Ghufran, M.; Hassan, S.; Khan, H.; Azam, S.; Rashid, U.

Pharm. Biol.

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In silico identification of promiscuous scaffolds as potential inhibitors of 1-deoxy-D-xylulose 5-phosphate reductoisomerase for treatment of falciparum malaria

2017

Wadood, A.; Ghufran, M.; Hassan, S.; Khan, H.; Azam, S.; Rashid, U.

Pharm. Biol.

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Effect of down-regulating 1-deoxy-D-xylulose-5-phosphate reductoisomerase by RNAi on growth and artemisinin biosynthesis in Artemisia annua L.

2018

Wang, C.; Lei, X.; Xia, J.; Wang, J.

Plant Growth Regul.

84

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763591

31279136

Evaluation, characterization, expression profiling, and functional analysis of DXS and DXR genes of Populus trichocarpa

2019

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Plant Physiol. Biochem.

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Evaluation, characterization, expression profiling, and functional analysis of DXS and DXR genes of Populus trichocarpa

2019

Xu, C.; Wei, H.; Movahedi, A.; Sun, W.; Ma, X.; Li, D.; Yin, T.; Zhuge, Q.

Plant Physiol. Biochem.

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763607

32272573

Cellular and subcellular compartmentation of the 2C-methyl-D-erythritol 4-phosphate pathway in the Madagascar periwinkle

2020

Guirimand, G.; Guihur, A.; Perello, C.; Phillips, M.; Mahroug, S.; Oudin, A.; Duge de Bernonville, T.; Besseau, S.; Lanoue, A.; Giglioli-Guivarch, N.; Papon, N.; St-Pierre, B.; Rodriguez-Concepcion, M.; Burlat, V.; Courdavault, V.

Plants (Basel)

9

462

763607

32272573

Cellular and subcellular compartmentation of the 2C-methyl-D-erythritol 4-phosphate pathway in the Madagascar periwinkle

2020

Guirimand, G.; Guihur, A.; Perello, C.; Phillips, M.; Mahroug, S.; Oudin, A.; Duge de Bernonville, T.; Besseau, S.; Lanoue, A.; Giglioli-Guivarch, N.; Papon, N.; St-Pierre, B.; Rodriguez-Concepcion, M.; Burlat, V.; Courdavault, V.

Plants (Basel)

9

462

763591

2 entries

763607

2 entries

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