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Ligand Mannotriose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.24

mannotriose + H2O = alpha-D-mannopyranose + mannobiose

136010

-

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

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Show Natural Product (5 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.24

mannotetraose + H2O = alpha-D-mannopyranose + mannotriose

136011, 136010

-

3.2.1.78

beta-galactomannan + H2O = mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

beta-glucomannan + H2O = mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

guar gum + H2O = mannose + mannobiose + mannotriose

208782, 0

-

3.2.1.78

ivory nut mannan + H2O = mannobiose + mannotriose

208814

-

3.2.1.78

4 entries

Substrate in Enzyme-catalyzed Reactions (15 results)

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Show Substrate (15 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.319

beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate = beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate

725502

-

2.4.1.319

beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate = beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate

725502

-

2.4.1.319

[(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate = [(1->4)-beta-D-mannosyl]4 + phosphate

725502

-

2.4.1.319

[(1->4)-beta-D-mannosyl]3 + phosphate = [(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate

725502

-

3.2.1.100

mannotriose + H2O = mannobiose + D-mannose

751110

-

3.2.1.100

mannotriose + H2O = mannobiose + mannose

135706

-

3.2.1.100

mannotriose + mannose = mannopentaose

135706

-

2.4.1.319

4 entries

3.2.1.100

3 entries

3.2.1.24

mannotriose + H2O = alpha-D-mannopyranose + mannobiose

136010

-

3.2.1.25

beta-D-Man-(1-4)-beta-D-Man-(1-4)-D-Man + H2O = ?

136045

-

3.2.1.25

mannotriose + H2O = 3 D-mannopyranose

748422

-

3.2.1.25

mannotriose + H2O = ?

136031, 136041, 665524, 136038

-

3.2.1.78

mannotriose + H2O = ?

731856, 751779

-

3.2.1.78

mannotriose + H2O = mannobiose + mannose

208785, 208798, 208796, 208795

-

3.2.1.78

mannotriose + H2O = mannose + mannobiose + mannotriose

750157

-

3.2.1.25

3 entries

3.2.1.78

3 entries

5.1.3.11

mannotriose = 4-O-beta-D-mannopyranosyl-O-beta-D-mannopyranosyl-D-glucose

747378

-

Product in Enzyme-catalyzed Reactions (49 results)

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Show Product (49 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.319

beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate = beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate

0

-

2.4.1.319

[(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate = [(1->4)-beta-D-mannosyl]3 + phosphate

0

-

2.4.1.319

[(1->4)-beta-D-mannosyl]4 + phosphate = [(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate

0

-

3.2.1.22

4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O = alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose

0

-

3.2.1.22

alpha-D-galactopyranosyl-(1-6)-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O = alpha-D-galactose + beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose

0

-

3.2.1.22

melibiose + melibiose = mannotriose + D-glucose

0

-

2.4.1.319

3 entries

3.2.1.22

3 entries

3.2.1.24

mannotetraose + H2O = alpha-D-mannopyranose + mannotriose

136011, 136010

-

3.2.1.78

mannohexaose + H2O = 2 mannotriose

0

-

3.2.1.78

beta-1,4-mannan + H2O = beta-1,4-mannotriose + beta-1,4-mannotetraose

0

-

3.2.1.78

4-methylumbelliferyl mannotrioside + H2O = 4-methylumbelliferone + mannotriose

0

-

3.2.1.78

beta-1,4-mannan + H2O = mannobiose + mannotriose

0

-

3.2.1.78

beta-1,4-mannan + H2O = mannose + mannobiose + mannotriose

0

-

3.2.1.78

beta-galactomannan + H2O = mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

beta-glucomannan + H2O = mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

galactomannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

glucomannan + H2O = mannobiose + mannotriose

0

-

3.2.1.78

guar gum + H2O = mannobiose + mannotriose

0

-

3.2.1.78

guar gum + H2O = mannobiose + mannotriose + mannose

0

-

3.2.1.78

guar gum + H2O = mannose + mannobiose + mannotriose

208782, 0

-

3.2.1.78

guar gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

insoluble mannan + H2O = mannotriose + mannotetraose + mannopentaose

0

-

3.2.1.78

ivory nut mannan + H2O = mannobiose + mannotriose

208814

-

3.2.1.78

ivory nut mannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

ivory nut mannan + H2O = mannotriose + mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

konjac glucomannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

konjac powder + H2O = mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose

0

-

3.2.1.78

konjaku flour + H2O = mannobiose + mannotriose + mannose

0

-

3.2.1.78

locust bean galactomannan + H2O = mannotriose + mannotetraose + mannopentaose

0

-

3.2.1.78

locust bean gum + H2O = mannobiose + mannotriose

0

-

3.2.1.78

locust bean gum + H2O = mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

locust bean gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

locust bean gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

locust bean gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose

0

-

3.2.1.78

locust bean gum + H2O = mannotriose + ?

0

-

3.2.1.78

locust bean gum galactomannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

0

-

3.2.1.78

mannan + H2O = mannotetraose + mannobiose + mannotriose

0

-

3.2.1.78

mannohexaose + H2O = mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

mannohexaose + H2O = mannotriose + mannotetraose + mannopentaose + mannobiose

0

-

3.2.1.78

mannopentaose + H2O = mannobiose + mannotriose

0

-

3.2.1.78

mannopentaose + H2O = mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

mannopentaose + H2O = mannose + mannobiose + mannotriose + mannotetraose

0, 208790, 208814

-

3.2.1.78

mannopentaose + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose

0

-

3.2.1.78

mannopentaose + H2O = mannotriose + mannobiose

0

-

3.2.1.78

mannopentaose + H2O = mannotriose + mannobiose + mannotetraose

0

-

3.2.1.78

mannotetraose + H2O = mannobiose + mannose + mannotriose

0, 208790, 208785, 208798, 208796

-

3.2.1.78

mannotetraose + H2O = mannobiose + mannotriose + mannopentaose

0

-

3.2.1.78

mannotetraose + H2O = mannose + mannobiose + mannotriose + mannotetraose

0

-

3.2.1.78

mannotetraose + H2O = mannotriose + mannobiose

0

-

3.2.1.78

mannotriose + H2O = mannose + mannobiose + mannotriose

0

-

3.2.1.78

42 entries

Inhibitor in Enzyme-catalyzed Reactions (5 results)

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Show Inhibitors (5 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.176

competitive

736028

-

3.2.1.91

effect on enzyme kinetics, overview

753457

-

3.5.1.52

-

288923

-

3.5.1.52

evidence for occupation of the carbohydrate-binding site

288927

-

3.5.1.52

2 entries

5.3.1.1

docking simulation

705563

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (5 results)

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Show Turnover Number (5 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.4.1.319

27.5

-

pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate

725502

2.4.1.319

27.5

-

pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction

725502

2.4.1.319

34.6

-

pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate

725502

2.4.1.319

34.6

-

pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction

725502

2.4.1.319

4 entries

3.2.1.25

166.7

-

pH 7.0, 37°C, wild-type enzyme

665524

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.319

7.94

-

pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate

725502

2.4.1.319

7.94

-

pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction

725502

2.4.1.319

13.3

-

pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate

725502

2.4.1.319

13.3

-

pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction

725502

2.4.1.319

4 entries

3.2.1.25

0.1

-

pH 7.0, 37°C, wild-type enzyme

665524

References & Links

Literature References (27)

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Show Reference (27 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

135706

7096283

Purification and characterization of a novel exo-beta-mannanase from Aeromonas sp. F-25

1982

Araki, T.; Kitamikado, M.

J. Biochem.

91

1181-1186

136010

-

Purification and some properties of alpha-mannosidase

1977

Katohda, S.; Sawaya, Y.; Asatsuma, K.; Suzuki, F.; Hayashibe, M.

Agric. Biol. Chem.

41

331-337

136011

338603

Purification and properties of alpha-mannosidase from baker's yeast

1977

Kaya, T.; Aikawa, M.; Matsumoto, T.

J. Biochem.

82

1443-1449

136031

-

Characterization of beta-mannosidase of an alkalophilic Bacillus sp.

1988

Akino, T.; Nakamura, N.; Horikoshi, K.

Agric. Biol. Chem.

52

1459-1464

136038

26672

Purification and characterization of beta-D-mannosidase and beta-N-acetyl-D-hexosaminidase of Tremella fuciformis

1978

Sone, Y.; Misaki, A.

J. Biochem.

83

1135-1144

136041

6478

beta-Mannosidase from the mushroom Polyporus sulfureus

1976

Wan, C.C.; Muldrey, J.E.; Li, S.C.; Li, Y.T.

J. Biol. Chem.

251

4384-4388

136045

4762411

Lysosomal beta-D-mannosidase of rat liver

1973

LaBadie, J.H.; Aronson, N.N.

Biochim. Biophys. Acta

321

603-614

208782

14323029

beta-Mannanases of fungi

1965

Reese, E.T.; Shibata, Y.

Can. J. Microbiol.

11

167-18

208785

-

Isolierung und Eigenschaften einer beta-1,4-Mannanase aus Aspergillus niger

1976

Yamazaki, N.; Sinner, M.; Dietrichs, H.H.

Holzforschung

30

101-109

208790

-

Purification and characterization of an endo-beta-mannanase from Aeromonas sp. F-25

1983

Araki, T.

J. Fac. Agric. Kyushu Univ.

27

89-98

208795

-

Purification and some properties of beta-mannanase from Penicillium purpurogenum

1987

Park, G.G.; Kusakabe, I.; Komatsu, Y.; Kobayashi, H.; Yasui, T.; Murakami, K.

Agric. Biol. Chem.

51

2709-2716

208796

-

Characterization of three beta-mannanases of an alkalophilic Bacillus sp.

1988

Akino, T.; Nakamura, N.; Horikoshi, K.

Agric. Biol. Chem.

52

773-779

208798

-

beta-D-Mannanase

1988

McCleary, B.V.

Methods Enzymol.

160

596-610

208814

8534110

Purification and characterization of an extracellular mannanase from a marine bacterium, Vibrio sp. strain MA-138

1995

Tamaru, Y.; Araki, T.; Amagoi, H.; Mori, H.; Morishita, T.

Appl. Environ. Microbiol.

61

4454-4458

288923

7535137

Does an animal peptide N:glycanase have the dual role as an enzyme and a carbohydrate binding protein?

1994

Suzuki, T.; Kitajima, K.; Inoue, S.; Inoue, Y.

Glycoconj. J.

11

469-476

288927

7797502

Carbohydrate-binding property of peptide:N-glycanase from mouse fibroblast L-929 cells as evaluated by inhibition and binding experiments using various oligosaccharides

1995

Suzuki, T.; Kitajima, K.; Inoue, Y.; Inoue, S.

J. Biol. Chem.

270

15181-15186

665524

15014076

Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A

2004

Dias, F.M.; Vincent, F.; Pell, G.; Prates, J.A.; Centeno, M.S.; Tailford, L.E.; Ferreira, L.M.; Fontes, C.M.; Davies, G.J.; Gilbert, H.J.

J. Biol. Chem.

279

25517-25526

705563

19423633

Interaction of triosephosphate isomerase from the cell surface of Staphylococcus aureus and alpha-(1->3)-mannooligosaccharides derived from glucuronoxylomannan of Cryptococcus neoformans

2009

Furuya, H.; Ikeda, R.

Microbiology

155

2707-2713

725502

23093406

Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase

2012

Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.

J. Biol. Chem.

287

42389-42399

731856

25466024

A thermophilic beta-mannanase from Neosartorya fischeri P1 with broad pH stability and significant hydrolysis ability of various mannan polymers

2015

Yang, H.; Shi, P.; Lu, H.; Wang, H.; Luo, H.; Huang, H.; Yang, P.; Yao, B.

Food Chem.

173

283-289

736028

25435507

Competitive inhibition of cellobiohydrolase I by manno-oligosaccharides

2015

Xin, D.; Ge, X.; Sun, Z.; Viikari, L.; Zhang, J.

Enzyme Microb. Technol.

68

62-68

747378

23801120

Characterization of a recombinant mannobiose 2-epimerase from Spirochaeta thermophila that is suggested to be a cellobiose 2-epimerase

2013

Park, C.; Kim, J.; Lee, S.; Kim, Y.; Kang, L.; Oh, D.

Biotechnol. Lett.

35

1873-1880

748422

-

Biochemical characterization of a recombinant thermostable beta-mannosidase from Thermotoga maritima with transglycosidase activity

2009

Zhang, M.; Jiang, Z.; Li, L.; Katrolia, P.

J. Mol. Catal. B

60

119-124

750157

27714555

Biocatalytic characterization of an endo-beta-1,4-mannanase produced by Paenibacillus sp. strain HY-8

2017

Kim, D.Y.; Chung, C.W.; Cho, H.Y.; Rhee, Y.H.; Shin, D.H.; Son, K.H.; Park, H.Y.

Biotechnol. Lett.

39

149-155

751110

29997257

Structural basis of exo-beta-mannanase activity in the GH2 family

2018

Domingues, M.N.; Souza, F.H.M.; Vieira, P.S.; de Morais, M.A.B.; Zanphorlin, L.M.; Dos Santos, C.R.; Pirolla, R.A.S.; Honorato, R.V.; de Oliveira, P.S.L.; Gozzo, F.C.; Murakami, M.T.

J. Biol. Chem.

293

13636-13649

751779

26567779

NMR analysis of the binding mode of two fungal endo-beta-1,4-mannanases from GH5 and GH26 families

2016

Marchetti, R.; Berrin, J.G.; Couturier, M.; Ul Qader, S.A.; Molinaro, A.; Silipo, A.

Org. Biomol. Chem.

14

314-322

753457

25435507

Competitive inhibition of cellobiohydrolase I by manno-oligosaccharides

2015

Xin, D.; Ge, X.; Sun, Z.; Viikari, L.; Zhang, J.

Enzyme Microb. Technol.

68

62-68

Links to other databases for Mannotriose

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