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Ligand 3-Hydroxyechinenone Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C4 0 H5 4 O2
3-Hydroxyechinenone
DFNMSBYEEKBETA-JZLJSYQFSA-N
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (2 results)
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3-hydroxyechinenone + acceptor + O2 = phoenicoxanthin + reduced acceptor
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3-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 = 4-ketozeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
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Product in Enzyme-catalyzed Reactions (2 results)
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echinenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 = 3-hydroxyechinenone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
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echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 = 3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
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Enzyme Kinetic Parameters
KM Value (2 results)
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0.0143
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30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.00182
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in 0.4 M Tris/HCl, pH 8.0, at 30°C
References & Links Literature References (3)
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The crtS gene of Xanthophyllomyces dendrorhous encodes a novel cytochrome-P450 hydroxylase involved in the conversion of beta-carotene into astaxanthin and other xanthophylls
2006
Alvarez, V.; Rodriguez-Saiz, M.; de la Fuente, J.L.; Gudina, E.J.; Godio, R.P.; Martin, J.F.; Barredo, J.L.
Fungal Genet. Biol.
43
261-272
Characterization of bacterial beta-carotene 3,3-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli
2006
Choi, S.; Matsuda, S.; Hoshino, T.; Peng, X.; Misawa, N.
Appl. Microbiol. Biotechnol.
72
1238-1246
Enzymic confirmation of reactions involved in routes to astaxanthin formation, elucidated using a direct substrate in vitro assay
1998
Fraser, P.D.; Shimada, H.; Misawa, N.
Eur. J. Biochem.
252
229-236
Links to other databases for 3-Hydroxyechinenone