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Ligand 2-hydroxy-ATP

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.6.1.56

2-hydroxy-ATP + H2O = 2-hydroxy-AMP + diphosphate

715460

-

Substrate in Enzyme-catalyzed Reactions (4 results)

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Show Substrate (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.6.1.13

2-hydroxy-ATP + H2O = ?

774215

-

3.6.1.56

2-hydroxy-ATP + H2O = 2-hydroxy-AMP + diphosphate

715460, 716361, 716299, 756056

-

3.6.1.56

2-hydroxy-rATP + H2O = 2-hydroxy-rAMP + diphosphate

758131

-

3.6.1.56

2 entries

5.6.2.6

2-hydroxy-ATP + H2O = 2-hydroxy-ADP + phosphate

713144

-

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

5.6.2.6

-

713144

-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (1 entry)

EC NUMBER

ENZYME 3D STRUCTURE

2.7.7.6

8sy5, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.6.1.56

4.7

-

pH 8.0, 30°C

716361

K_M Value (2 results)

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Show KM Value (2 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.6.1.56

0.0043

-

pH 8.0, 30°C

716361

3.6.1.56

0.0134

-

at pH 7.4, temperature not specified in the publication

758131

3.6.1.56

2 entries

K_i Value (1 result)

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Show KI Value (1 entry)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

5.6.2.6

2.4

-

pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction

713144

IC₅₀ Value (1 result)

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Show IC50 Value (1 entry)

EC NUMBER

IC50 VALUE

IC50 VALUE MAXIMUM

COMMENTARY

LITERATURE

5.6.2.6

3.3

-

pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction

713144

References & Links

Literature References (7)

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Show Reference (7 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

713144

20421212

Deciphering the molecular basis for nucleotide selection by the West Nile virus RNA helicase

2010

Despins, S.; Issur, M.; Bougie, I.; Bisaillon, M.

Nucleic Acids Res.

38

5493-5506

715460

11756418

A molecular basis for the selective recognition of 2-hydroxy-dATP and 8-oxo-dGTP by human MTH1

2002

Sakai, Y.; Furuichi, M.; Takahashi, M.; Mishima, M.; Iwai, S.; Shirakawa, M.; Nakabeppu, Y.

J. Biol. Chem.

277

8579-8587

716299

20542142

Programmed cell death triggered by nucleotide pool damage and its prevention by MutT homolog-1 (MTH1) with oxidized purine nucleoside triphosphatase

2010

Nakabeppu, Y.; Oka, S.; Sheng, Z.; Tsuchimoto, D.; Sakumi, K.

Mutat. Res.

703

51-58

716361

11139615

Human MTH1 protein hydrolyzes the oxidized ribonucleotide, 2-hydroxy-ATP

2011

Fujikawa, K.; Kamiya, H.; Yakushiji, H.; Nakabeppu, Y.; Kasai, H.

Nucleic Acids Res.

29

449-454

756056

29281266

Crystal structures and inhibitor interactions of mouse and dog MTH1 reveal species-specific differences in affinity

2018

Narwal, M.; Jemth, A.S.; Gustafsson, R.; Almloef, I.; Warpman Berglund, U.; Helleday, T.; Stenmark, P.

Biochemistry

57

593-603

758131

26999531

MTH1 substrate recognition - an example of specific promiscuity

2016

Nissink, J.W.; Bista, M.; Breed, J.; Carter, N.; Embrey, K.; Read, J.; Winter-Holt, J.J.

PLoS ONE

11

e0151154

774215

37343711

NUDT6 and NUDT9, two mitochondrial members of the NUDIX family, have distinct hydrolysis activities

2023

Debar, L.; Ishak, L.; Moretton, A.; Anoosheh, S.; Morel, F.; Jenninger, L.; Garreau-Balandier, I.; Vernet, P.; Hofer, A.; van den Wildenberg, S.; Farge, G.

Mitochondrion

71

93-103

Links to other databases for 2-hydroxy-ATP

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Show Links (1 entry)