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Ligand 2,4-dinitrophenyl beta-lactoside

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.176

2,4-dinitrophenyl beta-lactoside + H2O = 2,4-dinitrophenol + beta-lactose

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3.2.1.176

3,4-dinitrophenyl lactoside + H2O = 3,4-dinitrophenol + ?

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3.2.1.176

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.176

2.9

-

pH 5.7, 37°C, mutant D214N

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3.2.1.176

12.2

-

pH 5.7, 37°C, mutant E217Q

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3.2.1.176

104

-

pH 5.7, 37°C, wild-type enzyme

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3.2.1.176

3 entries

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.176

0.55

-

pH 5.7, 37°C, wild-type enzyme

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3.2.1.176

0.61

-

pH 5.7, 37°C, mutant E217Q

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3.2.1.176

0.67

-

pH 5.7, 37°C, mutant D214N

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380

-

25°C, pH 5, wild-type

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3.2.1.176

543

-

25°C, pH 5, E223S/A224H/L225V/T226A/D262G mutant

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3.2.1.176

5 entries

References & Links

Literature References (2)

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Show Reference (2 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

654486

11336632

Engineering of a glycosidase family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/A224H/L225V/T226A/D262G mutant

2001

Becker, D.; Braet, C.; Brumer, H.3rd.; Claeyssens, M.; Divne, C.; Fagerstrom, B.R.; Harris, M.; Jones, T.A.; Kleywegt, G.J.; Koivula, A.; Mahdi, S.; Piens, K.; Sinnott, M.L.; Stahlberg, J.; Teeri, T.T.; Underwood, M.; Wohlfahrt, G.

Biochem. J.

356

19-30

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Structure-reactivity studies of Trichoderma reesei cellobiohydrolase Cel7A

2004

Piens, K.; Stahlberg, J.; Nerinckx, W.; Teeri, T.T.; Claeyssens, M.

Am. Chem. Soc. Symp. Ser.

889

207-226

Links to other databases for 2,4-dinitrophenyl beta-lactoside

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Show Links (1 entry)

ChEBI

PubChem

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