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Ligand D-galactose 1-phosphate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.7.7.64

UTP + D-galactose 1-phosphate = diphosphate + UDP-galactose

704709

-

3.1.3.10

D-galactose 1-phosphate + H2O = D-galactose + phosphate

134579, 134582, 134583, 134585

-

Substrate in Enzyme-catalyzed Reactions (15 results)

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Show Substrate (15 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.3.9

galactose 1-phosphate + O2 = ? + H2O2

389882

-

2.7.7.12

UDP-glucose + D-galactose 1-phosphate = D-glucose 1-phosphate + UDP-galactose

703249

-

2.7.7.30

GTP + D-galactose 1-phosphate = diphosphate + GDP-galactose

661161

-

2.7.7.64

D-galactose 1-phosphate + dTTP = dTDP-D-galactose + diphosphate

737817

-

2.7.7.64

D-galactose 1-phosphate + dUTP = dUDP-D-galactose + diphosphate

737817

-

2.7.7.64

D-galactose 1-phosphate + TTP = TDP-D-galactose + diphosphate

737765

-

2.7.7.64

D-galactose 1-phosphate + UTP = UDP-D-galactose + diphosphate

704709, 737765, 737817, 738819

-

2.7.7.64

D-galactose 1-phosphate + UTP = UDP-galactose + diphosphate

721524, 691365

-

2.7.7.64

UTP + D-galactose 1-phosphate = diphosphate + UDP-galactose

704709, 669277, 670505, 668028

-

2.7.7.64

6 entries

3.1.3.10

D-galactose 1-phosphate + H2O = D-galactose + phosphate

134579, 134582, 134583, 134585

-

3.1.3.2

D-galactose 1-phosphate + H2O = D-galactose + phosphate

677425

-

3.1.3.23

D-galactose 1-phosphate + H2O = D-galactose + phosphate

714982

-

3.1.3.25

D-galactose 1-phosphate + H2O = D-galactose + phosphate

750664

-

3.1.3.58

D-galactose-1-phosphate + H2O = D-galactose + phosphate

646416

-

3.1.3.93

D-galactose 1-phosphate + H2O = D-galactose + phosphate

716596

-

Product in Enzyme-catalyzed Reactions (6 results)

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Show Product (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.7.1.157

ATP + D-galactose = ADP + D-galactose 1-phosphate

0

-

2.7.1.162

ATP + D-galactose = ADP + D-galactose 1-phosphate

0

-

2.7.1.6

ATP + D-galactose = ADP + D-galactose 1-phosphate

0

-

2.7.7.37

GDP-galactose + phosphate = GDP + D-galactose 1-phosphate

0

-

2.7.7.37

UDP-galactose + phosphate = UDP + D-galactose 1-phosphate

0

-

2.7.7.37

2 entries

3.6.1.45

UDP-galactose = UMP + galactose 1-phosphate

209944

-

Activator in Enzyme-catalyzed Reactions (2 results)

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Show Activating Compound (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

5.1.3.2

activation of minor enzyme form, no effect on major enzyme form

2325

-

5.1.3.2

stimulates

2315

-

5.1.3.2

2 entries

Inhibitor in Enzyme-catalyzed Reactions (3 results)

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Show Inhibitors (3 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.200

about 40% inhibition at 20 mM

762130

-

2.7.1.6

-

641711, 641719

-

2.7.1.6

-

704761

-

2.7.1.6

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (8 results)

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Show Turnover Number (8 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.7.7.30

0.23

-

pH 7.0, 37°C

661161

2.7.7.64

-999

-

Kcat/KM: 490000 1/M/sec, pH 7, 35°C

691365

2.7.7.64

35.2

-

co-substrate: UTP, pH 7.6, 37°C

721524

2.7.7.64

133

-

pH 7, 35°C

691365

2.7.7.64

219

-

forward reaction, pH 7.8, 25°C

704709

2.7.7.64

4 entries

3.1.3.10

0.0348

-

-

134585

3.1.3.23

4.28

-

pH 7.0, 37°C

714982

3.1.3.25

2.4

-

pH 9.0, 25°C

750664

K_M Value (9 results)

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Show KM Value (9 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.7.7.30

0.677

-

pH 7.0, 37°C

661161

2.7.7.64

0.09

-

cosubstrate UTP, S0.5 value, pH 8.0, 37°C

737765

2.7.7.64

0.27

-

pH 7, 35°C

691365

2.7.7.64

0.58

-

pH 7.0, 35°C

669277

2.7.7.64

0.818

-

co-substrate: UTP, pH 7.6, 37°C

721524

2.7.7.64

0.86

-

forward reaction, pH 7.8, 25°C

704709

2.7.7.64

5 entries

3.1.3.10

85.4

-

-

134585

3.1.3.23

40.74

-

pH 7.0, 37°C

714982

3.1.3.25

0.45

-

pH 9.0, 25°C

750664

K_i Value (2 results)

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Show KI Value (2 entries)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.7.1.6

2

-

37°C

641719

2.7.1.6

3.5

-

pH 7.3, 25°C, competitive inhibition with galactose as the variable substrate

641711

2.7.1.6

2 entries

IC₅₀ Value (1 result)

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Show IC50 Value (1 entry)

EC NUMBER

IC50 VALUE

IC50 VALUE MAXIMUM

COMMENTARY

LITERATURE

2.7.1.6

16.8

-

-

704761

References & Links

Literature References (28)

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Show Reference (28 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

2315

-

Purification and properties of UDP-galactose 4-epimerase from Bifidobacterium bifidum

1978

Lee, L.J.; Kimura, A.; Tochikura, T.

Agric. Biol. Chem.

42

731-737

2325

4348173

Two forms of UDPglucose-4-epimerase from mammalian liver

1973

Ray, M.; Bhaduri, A.

Biochim. Biophys. Acta

302

129-134

134579

1648777

Utilization of exogenous glucose-1-phosphate as a source of carbon or phosphate by Escherichia coli K12: respective roles of acid glucose-1-phosphatase, hexose-phosphate permease, phosphoglucomutase and alkaline phosphatase

1991

Pradel, E.; Boquet, P.L.

Res. Microbiol.

142

37- 45

134582

13249953

A hexose-1-phosphatase in silkworm blood

1955

Faulkner, P.

Biochem. J.

60

590-596

134583

-

Metabolic role of the multiple forms of glucose-1-phosphatase, NAD+-and NADP+-dependent sorbitol dehydrogenases, acid phosphatase, and glucose-6-phosphate dehydrogenase in tissues and organs of the silkworm

1997

Konichev, A.S.

Biochemistry

62

543-550

134585

9972248

Isolation and properties of glucose-1-phosphatase from mycelia of Pholiota nemako

1998

Joh, T.; Yazaki, J.; Suzuki, K.; Hayakawa, T.

Biosci. Biotechnol. Biochem.

62

2251-2253

209944

5337593

Uridine diphosphate sugar hydrolase. Purification of enzyme and protein inhibitor

1967

Glaser, L.; Melo, A.; Paul, R.

J. Biol. Chem.

242

1944-1954

389882

13863403

The D-galactose oxidase of Polyporus circinatus

1962

Avigad, G.; Amaral, D.; Asensio, C.; Horecker, B.L.

J. Biol. Chem.

237

2736-2743

641711

6617655

Galactokinase of Vicia faba seeds

1983

Dey, P.M.

Eur. J. Biochem.

136

155-159

641719

5028074

Purification and kinetic properties of galactokinase from human placenta

1972

Srivastava, S.K.; Blume, K.G.; Van Loon, C.; Beutler, E.

Arch. Biochem. Biophys.

150

191-198

646416

2993253

Characterization of a membrane-regulated sugar phosphate phosphohydrolase from Lactobacillus casei

1985

London, J.; Hausman, S.Z.; Thompson, J.

J. Bacteriol.

163

951-956

661161

16086588

Substrate discrimination by the human GTP fucose pyrophosphorylase

2005

Quirk, S.; Seley, K.L.

Biochemistry

44

10854-10863

668028

9889080

Biochemical characterization of a UDP-sugar pyrophosphorylase from Thermus caldophilus GK24

1999

Kim, J.S.; Koh, S.; Shin, H.J.; Lee, D.S.; Lee, S.Y.

Biotechnol. Appl. Biochem.

29

11-17

669277

15326166

UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts

2004

Kotake, T.; Yamaguchi, D.; Ohzono, H.; Hojo, S.; Kaneko, S.; Ishida, H.K.; Tsumuraya, Y.

J. Biol. Chem.

279

45728-45736

670505

100107392

Biosynthesis of UDP-glucuronic acid in developing soybean embryos: Possible role of UDP-sugar pyrophosphorylase

2006

Litterer, L.A.; Plaisance, K.L.; Schnurr, J.A.; Storey, K.K.; Jung, H.J.G.; Gronwald, J.W.; Somers, D.A.

Physiol. Plant.

128

200-211

677425

-

Purification and characterization of three low-molecular-weight acid phosphatases from peanut (Arachis hypogaea) seedlings

2006

Gonnety, J.T.; Niamke, S.; Faulet, B.M.; Jean-Parfait, E.; Kouadio, N.; Kouame, L.P.

Afr. J. Biotechnol.

5

035-044

691365

17341835

Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis

2007

Kotake, T.; Hojo, S.; Yamaguchi, D.; Aohara, T.; Konishi, T.; Tsumuraya, Y.

Biosci. Biotechnol. Biochem.

71

761-771

703249

19616531

Positive correlation between galactose-1-phosphate uridyltransferase (GALT) and UDP-galactose-4-epimerase (GALE) activities

2009

Prodan-Zitnik, I.; Karas-Kuzelicki, N.; Lukac-Bajalo, J.

Clin. Biochem.

42

1561-1564

704709

19906649

Leishmania UDP-sugar pyrophosphorylase: the missing link in galactose salvage?

2010

Damerow, S.; Lamerz, A.C.; Haselhorst, T.; Fuehring, J.; Zarnovican, P.; von Itzstein, M.; Routier, F.H.

J. Biol. Chem.

285

878-887

704761

19433218

Functional studies of rat galactokinase

2009

Chu, X.; Li, N.; Liu, X.; Li, D.

J. Biotechnol.

141

142-146

714982

21575135

Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis

2011

Godinho, L.M.; de Sa-Nogueira, I.

FEBS J.

278

2511-2524

716596

19339506

VTC4 is a bifunctional enzyme that affects myoinositol and ascorbate biosynthesis in plants

2009

Torabinejad, J.; Donahue, J.L.; Gunesekera, B.N.; Allen-Daniels, M.J.; Gillaspy, G.E.

Plant Physiol.

150

951-961

721524

20482518

Identification of a novel UDP-sugar pyrophosphorylase with a broad substrate specificity in Trypanosoma cruzi

2010

Yang, T.; Bar-Peled, M.

Biochem. J.

429

533-543

737765

25316289

The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate

2015

Ebrecht, A.C.; Asencion Diez, M.D.; Piattoni, C.V.; Guerrero, S.A.; Iglesias, A.A.

Biochim. Biophys. Acta

1850

88-96

737817

23707255

Biosynthesis of nucleotide sugars by a promiscuous UDP-sugar pyrophosphorylase from Arabidopsis thaliana (AtUSP)

2013

Liu, J.; Zou, Y.; Guan, W.; Zhai, Y.; Xue, M.; Jin, L.; Zhao, X.; Dong, J.; Wang, W.; Shen, J.; Wang, P.G.; Chen, M.

Bioorg. Med. Chem. Lett.

23

3764-3768

738819

-

Characterization of uridine diphosphate-sugar pyrophosphorylase from Populus deltoids

2013

Kim, B.; Ahn, J.

J. Korean Soc. Appl. Biol. Chem.

56

525-531

750664

25620968

Characterization of the inositol monophosphatase gene family in Arabidopsis

2015

Nourbakhsh, A.; Collakova, E.; Gillaspy, G.

Front. Plant Sci.

5

725

762130

28011870

Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants

2017

Hartman, M.; Figueroa, C.; Arias, D.; Iglesias, A.

Plant Cell Physiol.

58

145-155

Links to other databases for D-galactose 1-phosphate

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Show Links (1 entry)

ChEBI

PubChem