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BRENDA support

Ligand glutaraldehyde

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Basic Ligand Information

Molecular Structure
Picture of glutaraldehyde (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C5H8O2
glutaraldehyde
SXRSQZLOMIGNAQ-UHFFFAOYSA-N
Synonyms:
1,5-pentanedione, Glutaral, Glutardialdehyde, glutaric dialdehyde

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (33 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
glutaraldehyde + NADPH = ? + NADP+
show the reaction diagram
-
glutaraldehyde + NADPH = ? + NADP+
show the reaction diagram
-
glutaraldehyde + NADH = ?
show the reaction diagram
-
glutaraldehyde + NADH + H+ = ? + NAD+
show the reaction diagram
-
glutaraldehyde + CoA + NAD+ = glutaryl-CoA + NADH
show the reaction diagram
-
glutaraldehyde + NAD+ + H2O = glutarate semialdehyde + NADH
show the reaction diagram
-
glutaraldehyde + NAD+ + H2O = glutarate + NADH
show the reaction diagram
-
glutaraldehyde + NAD(P)+ + H2O = glutarate + NAD(P)H + H+
show the reaction diagram
-
glutaraldehyde + NAD+ + H2O = glutarate + NADH
show the reaction diagram
-
glutaraldehyde + NADP+ + H2O = glutarate + NADPH + H+
show the reaction diagram
-
glutaraldehyde + NAD+ = glutaric acid + NADH
show the reaction diagram
-
glutaraldehyde + NAD+ = glutarate + NADH
show the reaction diagram
-
glutaric dialdehyde + H2O + oxidized benzyl viologen = ? + reduced benzyl viologen + H+
show the reaction diagram
-
glutaraldehyde + H2O + benzylviologen = glutarate + reduced benzylviologen
show the reaction diagram
-
D-ribulose 5-phosphate + glutaraldehyde = ?
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
1,5-pentanediol + O2 = 1,5-pentanedione + H2O2
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
treating with 0.5% glutaraldehyde solution, the activity of the immobilized enzyme is at maximum
-
substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
-

Inhibitor in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
71% relative activity in the presence of 10 mM glutaraldehyde
-
10 mM, 83% inhibition
-
does not support enzyme stability, but inhibits the enzyme activity
-
1%, 65% inactivation
-
0.4%, 57% loss of activity
-
about 5% residual activity at 2 mM
-
inhibits the enzyme during immobilization at concentrations above 0.2%
-
22% inhibition at 4 mM
-
0.05 mM, 58% inhibition
-

3D Structure of Enzyme-Ligand-Complex (PDB) (32 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (6 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
93.13
-
pH 6, 60°C
12.3
-
1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C
3.92
-
pH 8.4, 28°C, cosubstrate NADP+
42000
-
80°C, pH 8.4

KM Value (16 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.67
-
pH 6, 60°C
0.0163
-
1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C
0.11
-
pH 8.4, 28°C, cosubstrate NADP+
1.7
-
-
0.8
-
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
0.8
-
80°C, pH 8.4
0.084
-
-

References & Links

Links to other databases for glutaraldehyde

ChEBI
PubChem
ChEBI
PubChem