Ligand chitosan

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Basic Ligand Information

Molecular Structure
Picture of chitosan (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C50H90N8O34R2
chitosan
CCTIJUAYBCXQDH-XCONQBMMSA-N

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
chitosan + H2O = ?
show the reaction diagram
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chitosan + H2O = N-acetyl-beta-D-glucosamine
show the reaction diagram
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chitosan + H2O = low-molecular mass chitosan + chito-oligomeric-monomeric mixture
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (41 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
chitosan + H2O = N-acetyl-D-glucosamine + ?
show the reaction diagram
-
chitosan + H2O = N-acetyl-beta-D-glucosamine
show the reaction diagram
-
chitosan + H2O = ?
show the reaction diagram
-
chitosan + H2O = ?
show the reaction diagram
-
chitosan + H2O = ?
show the reaction diagram
-
chitosan + H2O = N-acetyl-D-glucosamine + ?
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
induces expression
-
the beta-CGTase and gamma-CGTase activities increase as the chitosan concentrations increases to 2% (w/v)
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treatment leads to increased enzymic activity in cortex and cork tissues, with maximum specific activity after 12 h, and accumulation of phenolic compounds including 2-hydroxy-4-methoxybenzaldehyde
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Inhibitor in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
conjugated to the enzyme, with 81-93% laccase being conjugated, a moderate activity loss of 16-28% occurs in conjugation solution, a second severe activity loss of 63-78% occurs during a cycle of phase change consisting of precipitation, centrifugation and re-dissolution of the enzymechitosan conjugates, the chitosan molecular size has little effect, overview
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various sizes of chitosans (CTSN, CTSN-P, CTSN-B, and CTSN-S) prepared as low molecular weight chitosan show significant inhibitory effect on enzyme DSase activity, antimicrobial activity of CTSNs toward Streptococcus mutans, overview
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the Haldane kinetic model adequately describes the dynamic behavior of the chitosan enzymolysis by papain. When the initial chitosan concentration is above 8.0 g/l, the papain is overloaded and exhibits significant inhibition
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molecular weight about 6 kDA, complex formation with enzyme due to establishment of 5-6 ion pairs
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (37 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.0025
-
-
28.3
-
-

KM Value (18 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
-999
-
Km-value (mg/ml): 0.104 (wild-type enzyme), 0.221 (mutant enzyme E409Q), 0.140 (mutant enzyme E415Q), 0.352 (mutant enzyme D513N), pH 4.0

Ki Value (9 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

References & Links

Links to other databases for chitosan

ChEBI
PubChem
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PubChem