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Ligand 2-amino-6-mercapto-7-methylpurine ribonucleoside

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.2.1

2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate = ?

489710, 489711

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2.4.2.1

2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate = alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine

662951, 706743, 701915, 489711

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2.4.2.1

2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate = alpha-D-ribose 1-phosphate + ?

663288

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2.4.2.1

3 entries

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.4.2.1

in absence of phosphate the enzyme catalyzes a slow hydrolysis, which is accompanied by inactivation of the enzyme

489710

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.4.2.1

17.3

-

-

701915

K_M Value (2 results)

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Show KM Value (2 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.2.1

0.029

-

-

701915

2.4.2.1

0.1425

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pH 7.6

662951

2.4.2.1

2 entries

References & Links

Literature References (6)

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Show Reference (6 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

489710

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Purine nucleoside phosphorylase-catalyzed, phosphate-independent hydrolysis of 2-amino-6-mercapto-7-methylpurine ribonucleoside

1999

Cheng, J.; Farutin, V.; Wu, Z.; Jacob-Mosier, G.; Riley, B.; Hakimi, R.; Cordes, E.H.

Bioorg. Chem.

27

307-325

489711

11337031

Purine nucleoside phosphorylases: properties, functions, and clinical aspects

2000

Bzowska, A.; Kulikowska, E.; Shugar, D.

Pharmacol. Ther.

88

349-425

662951

15532538

Purine nucleoside phosphorylase activity in rat cerebrospinal fluid

2004

Silva, R.G.; Santos, D.S.; Basso, L.A.; Oses, J.P.; Wofchuk, S.; Portela, L.V.; Souza, D.O.

Neurochem. Res.

29

1831-1835

663288

12509998

Cloning, overexpression, and purification of functional human purine nucleoside phosphorylase

2003

Silva, R.G.; Carvalho, L.P.; Oliveira, J.S.; Pinto, C.A.; Mendes, M.A.; Palma, M.S.; Basso, L.A.; Santos, D.S.

Protein Expr. Purif.

27

158-164

701915

19416718

Substrate specificity and kinetic mechanism of purine nucleoside phosphorylase from Mycobacterium tuberculosis

2009

Ducati, R.G.; Santos, D.S.; Basso, L.A.

Arch. Biochem. Biophys.

486

155-164

706743

19929278

Effects of some modulators on purine nucleoside phosphorylase activity in myocardial tissue

2010

Aberg, A.M.; Ronquist, G.; Haney, M.; Waldenstrom, A.

Scand. J. Clin. Lab. Invest.

70

8-14

Links to other databases for 2-amino-6-mercapto-7-methylpurine ribonucleoside

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Show Links (1 entry)

ChEBI

PubChem

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