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Ligand (7R)-5,6,7,8-tetrahydrobiopterin Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C9 H1 5 N5 O3
(7R)-5,6,7,8-tetrahydrobiopterin
UUJCZDAMSPXYEU-RPDRRWSUSA-N
(7R)-5,6,7,8-tetrahydropterin, (7R)-tetrahydrobiopterin
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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L-phenylalanine + (7R)-5,6,7,8-tetrahydrobiopterin + O2 = L-tyrosine + 4a-hydroxytetrahydrobiopterin
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Enzyme Cofactor/Cosubstrate (1 result)
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8fold lower affinity and activity compared with 6(R)BH4
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Inhibitor in Enzyme-catalyzed Reactions (4 results)
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0.001 mM, 50% inhibition at 0.5 mM phenylalanine, 0.004 mM, 50% inhibition at 0.1 mM phenylalanine, recombinant enzyme
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slight inhibition, synthetic pathway, overview, conformational structure by NMR
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recombinant pinal enzyme, 2.1 mM, 50% inhibition
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Enzyme Kinetic Parameters
KM Value (2 results)
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2.1
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substrate tryptophan
Ki Value (1 result)
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References & Links Literature References (3)
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Cloning and expression of recombinant human pineal tryptophan hydroxylase in Escherichia coli: purification and characterization of the cloned enzyme
1999
Kowlessur, D.; Kaufman, S.
Biochim. Biophys. Acta
1434
317-330
Recombinant human phenylalanine hydroxylase: novel regulatory and structural properties
1996
Kowlessur, D.; Citron, B.A.; Kaufman, S.
Arch. Biochem. Biophys.
333
85-95
Specific interaction of the diastereomers 7(R)- and 7(S)-tetrahydrobiopterin with phenylalanine hydroxylase: implications for understanding primapterinuria and vitiligo
2006
Pey, A.L.; Martinez, A.; Charubala, R.; Maitland, D.J.; Teigen, K.; Calvo, A.; Pfleiderer, W.; Wood, J.M.; Schallreuter, K.U.
FASEB J.
20
2130-2132
Links to other databases for (7R)-5,6,7,8-tetrahydrobiopterin