Ligand N1-acetylspermine

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Basic Ligand Information

Molecular Structure
Picture of N1-acetylspermine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C12H28N4O
N1-acetylspermine
GUNURVWAJRRUAV-UHFFFAOYSA-N
Synonyms:
1-N-acetylspermine, N1-acetyl-spermine
Pathway Source
Pathways


Show all pahtways known for Show all BRENDA pathways known for N1-acetylspermine

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
acetyl-CoA + N1-acetylspermine = CoA + N1,N12-diacetylspermine
show the reaction diagram
N1-acetylspermine + H2O = acetate + spermine
-

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
N1-acetylspermine + O2 + H2O = 1,3-diaminopropane + H2O2 + ?
-
show the reaction diagram
N1-acetylspermine + O2 + H2O = 1,3-diaminopropane + H2O2 + ?
-
show the reaction diagram
acetyl-CoA + N1-acetylspermine = CoA + N1,N12-diacetylspermine

Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (1 result)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
both stimulatory and inhibitory effects at the kinetic phase in the presence of the factors washable from ribosomes, depending on ligand concentration
-

Inhibitor in Enzyme-catalyzed Reactions (3 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
non-competitive
-
poor competitive inhibitor
-
both stimulatory and inhibitory effects at the kinetic phase in the presence of the factors washable from ribosomes, depending on ligand concentration
-

3D Structure of Enzyme-Ligand-Complex (PDB) (8 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (33 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
37°C, pH 6.5
0.025
-
-
17
-
37°C, pH 7.5
0.084
-
pH 8.0
31.7
-
pH 7.6, 30°C
4.53
-
pH 6.5, 30°C
20.5
-
pH 7.0, 37°C
18.2
-
splice variant SMO5
0.28
-
SMO/PAOh1
0.28
-
pH 9.0, 20°C
47
-
pH 8.0, temperature not specified in the publication
0.2
-
pH 8.0, 25°C, recombinant AtPAO1
0.2
-
pH 8.0
0.2
-
pH 7.5, temperature not specified in the publication
0.014
-
pH 10.0, 30°C, recombinant mutant H64N
4.1
-
37°C, pH 6.5
0.025
-
37°C, pH 7.0
0.02
-
pH 9.0, 25°C, recombinant wild-type Fms1
15.1
-
pH 9.0, 25°C, recombinant mutant N195A
8.1
-
pH 9.0, 25°C, recombinant mutant D94N
9.7
-
37°C, pH 7.5
0.084
-
pH 8.5, 30°C
0.022
-
pH 10.0, 30°C, recombinant mutant H64A
3.1
-
pH 7.5, 25°C, recombinant AtPAO4
0.014
-
pH 10.0, 30°C, recombinant mutant H64Q
4.5
-
pH 10.0, 30°C, recombinant wild-type enzyme
49
-
pH 7.5, 25°C, recombinant AtPAO2
0.8
-
pH 7.5, temperature not specified in the publication
pH 7.5, 25°C, recombinant AtPAO3
0.02
-
pH 7.5, 37°C, recombinant AtPAO3
2
-
pH 9
0.12
-
pH 8.0, 22°C, recombinant enzyme
0.011
-

KM Value (48 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
-
0.0011
-
37°C, pH 6.5
0.0218
-
37°C, pH 8.0, wild-type enzyme
0.0369
-
pH 8.0
0.00085
-
37°C, pH 7.5
0.0019
-
37°C, pH 8.0, mutant enzyme T467Y
0.046
-
37°C, pH 8.0, mutant enzyme T467S
0.058
-
37°C, pH 8.0, mutant enzyme K315M
0.043
-
37°C, pH 8.0, mutant enzyme H69E
0.062
-
37°C, pH 8.0, mutant enzyme H69Q
0.071
-
-
0.062
-
pH 7.0, 37°C
0.0701
-
pH 6.5, 30°C
0.0594
-
37°C, pH 8
0.338
-
pH 8.0, temperature not specified in the publication
0.47
-
pH 7.2, 37°C
2.125
-
pH 9.0, 20°C
0.00158
-
pH 8.0
0.47
-
pH 8.0, 25°C, recombinant AtPAO1
0.47
-
37°C, pH 8.0
0.051
-
pH 8.3, 25°C
0.49
2
pH 8.5, 30°C
0.0359
-
pH 9.0, 25°C, recombinant mutant D94N
0.03
-
pH 7.5, temperature not specified in the publication
pH 7.5, 37°C, recombinant AtPAO3
0.02
-
pH 7.5, 25°C, recombinant AtPAO3
2
-
pH 7.5, 25°C, recombinant AtPAO2
0.233
-
pH 9.0, 25°C, recombinant mutant N195A
0.104
-
pH 9.0, 25°C, recombinant wild-type Fms1
0.011
-
pH 10.0, 30°C, recombinant wild-type enzyme
0.21
-
37°C, pH 6.5
0.0218
-
37°C, pH 7.0
2
-
37°C, pH 7.5
0.0019
-
37°C, pH 8.0, mutant enzyme H69E
0.062
-
37°C, pH 8.0, mutant enzyme H69Q
0.071
-
37°C, pH 8.0, mutant enzyme K315M
0.043
-
37°C, pH 8.0, mutant enzyme T467S
0.058
-
37°C, pH 8.0, mutant enzyme T467Y
0.046
-
37°C, pH 8.0, wild-type enzyme
0.0369
-
pH 10.0, 30°C, recombinant mutant H64A
0.05
-
pH 10.0, 30°C, recombinant mutant H64N
0.24
-
pH 10.0, 30°C, recombinant mutant H64Q
0.053
-
pH 7.2, 37°C
2.125
-
pH 9
0.295
-
-
0.03
-
pH 8.0, 22°C, recombinant enzyme
0.18
-
-
0.016
-

Ki Value (1 result)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE

References & Links

Links to other databases for N1-acetylspermine

ChEBI
PubChem
-
PubChem