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Ligand 1-kestose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.243

1-kestose + 3 sucrose = 3 neokestose + H2O

706297

-

2.4.1.243

4 1-kestose = sucrose + 1G-kestotetraose + 6G-kestotetraose + H2O

706297

-

2.4.1.243

2 entries

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

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Show Natural Product (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.99

sucrose + sucrose = D-glucose + 1-kestose

0

-

2.4.1.99

sucrose + sucrose = D-glucose + 1F-beta-D-fructosylsucrose

0

-

2.4.1.99

2 sucrose = D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside

0

-

2.4.1.99

3 entries

Substrate in Enzyme-catalyzed Reactions (58 results)

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Show Substrate (58 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.10

1-kestotriose + sucrose = 1,6-kestotetraose + ?

736970

-

2.4.1.100

1-kestose + 1-kestose = 1,1-nystose + 1,1,1-logose

488350, 488351, 670618, 488355, 488349

-

2.4.1.100

1-kestose + 1-kestose = D-sucrose + 1,1-nystose

706297

-

2.4.1.100

1-kestose + 1-kestose = nystose

757341

-

2.4.1.100

1-kestose + 6-kestose = 1,6-nystose + 6,1-nystose

488349

-

2.4.1.100

1-kestose = 1,1-nystose + ?

706470

-

2.4.1.100

1-kestose = 1F,6G-di-beta-D-fructofuranosylsucrose + nystose

657436, 660021

-

2.4.1.100

1-kestose = ?

660229

-

2.4.1.100

6-kestose + 1-kestose + sucrose = mixture of tetrasacharides of beta-2,6- and beta-2,1-linked fructans

488346

-

2.4.1.100

sucrose + 1-kestose = 1,1-nystose

488349, 488347, 488355

-

2.4.1.100

sucrose + 1-kestose = 1-kestose + 6G-kestose + tetrasaccharide

488350

-

2.4.1.100

1-kestotriose + 6G-kestotriose = 1,1-kestotetraose + ?

736970

-

2.4.1.100

1-kestotriose + sucrose = 1,1-nystose

704883

-

2.4.1.100

1-kestotriose = 1,1-kestotetraose + ?

700484

-

2.4.1.100

1-kestotriose = inulin

755836, 660328

-

2.4.1.100

2 1-kestose = 1-nystose + ?

755053

-

2.4.1.100

2 1-kestose = inulin + H2O

660229

-

2.4.1.243

1-kestose + 1-kestose = ?

664531, 666472

-

2.4.1.243

1-kestose + 1-kestose = D-sucrose + 1G-kestotetraose + 6G-kestotetraose

706297

-

2.4.1.243

1-kestose + 3 sucrose = 3 neokestose + H2O

688638, 689242, 706297, 686909, 666595, 723561

-

2.4.1.243

1-kestose + sucrose = 1,1-kestotetraose + 1,6-kestotetraose

722874

-

2.4.1.243

1-kestose + sucrose = 1,1-kestotetraose + 1G-kestotetraose + 6G-kestotetraose

666471

-

2.4.1.243

1-kestose + sucrose = 6G,6-kestotetraose

722874

-

2.4.1.243

1-kestose + sucrose = D-sucrose + neokestose

706297

-

2.4.1.243

1-kestose = ?

723561

-

2.4.1.243

nystose + 1-kestose = ?

666472

-

2.4.1.243

1-kestotriose + sucrose = 6G-kestotetraose + 1G-kestotetraose

736970

-

2.4.1.243

1F(1-beta-D-fructofuranosyl)sucrose + sucrose = 1F,6G-di-beta-D-fructofuranosylsucrose + ?

660021

-

2.4.1.243

4 1-kestose = sucrose + 1G-kestotetraose + 6G-kestotetraose + H2O

706297

-

2.4.1.100

16 entries

2.4.1.243

12 entries

2.4.1.9

sucrose + kestose = ?

675837

-

2.4.1.99

1-kestose + 1-kestose = 1,1-nystose + sucrose

489636

-

2.4.1.99

1-kestose + 1-kestose = D-fructose + sucrose + ?

660020

-

2.4.1.99

1-kestose + 1-kestose = fructose + nystose

757277

-

2.4.1.99

1-kestose = nystose + D-fructose

689660

-

2.4.1.99

sucrose + 1-kestose = D-glucose + ?

660020

-

2.4.1.99

2 1-kestose = 1-nystose + ?

755053

-

3.2.1.153

1-kestose + H2O = ?

657048, 208825, 682147

-

3.2.1.153

1-kestose + H2O = beta-D-fructose + sucrose

753837

-

3.2.1.153

1-kestose + H2O = D-fructose + sucrose

700708

-

3.2.1.153

1-kestotriose + H2O = ?

716041, 208824, 681133, 706391

-

3.2.1.154

1,1-kestose + H2O = ?

657381

-

3.2.1.154

1-kestose + H2O = ?

657381, 657006

-

3.2.1.154

1-kestotriose + H2O = ?

732665, 715762, 716041

-

3.2.1.26

1-kestose + H2O = ?

680989, 136062, 681151, 680952, 730034

-

3.2.1.26

1-kestose + H2O = D-fructose + ?

708103, 714490, 679597, 716998, 750234

-

3.2.1.26

1-kestose + H2O = D-fructose + D-glucose + sucrose

730730

-

3.2.1.26

alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose + H2O = D-glucose + ?

695761

-

3.2.1.64

1-kestose + H2O = ?

651408

-

3.2.1.64

1-kestose + H2O = sucrose + beta-D-fructose + alpha-D-glucose

136755

-

2.4.1.99

6 entries

3.2.1.153

4 entries

3.2.1.154

3 entries

3.2.1.26

4 entries

3.2.1.64

2 entries

3.2.1.65

1-kestose + H2O = D-fructose + sucrose

655294

-

3.2.1.7

1-kestose + H2O = ?

136822

-

3.2.1.80

1,1-kestose + H2O = beta-D-fructose + sucrose

657381

-

3.2.1.80

1-kestose + H2O = ?

682143, 681161, 682426, 714715, 751867

-

3.2.1.80

1-kestose + H2O = beta D-fructofuranose + sucrose

682145, 657048

-

3.2.1.80

1-kestose + H2O = beta-D-fructose + sucrose

668724, 682143, 682145, 682147, 753837, 754296, 657381

-

3.2.1.80

1-kestotriose + H2O = ?

681133, 715762, 716041

-

3.2.1.80

1-kestotriose + H2O = beta-D-fructose + sucrose

750665

-

3.2.1.80

6 entries

4.2.2.18

1-kestose = di-D-fructofuranose 1,2':2,3'-dianhydride + D-glucose

714496

-

Product in Enzyme-catalyzed Reactions (41 results)

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Show Product (41 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.10

sucrose + sucrose = 1-kestose + 6-kestose + neo-kestose + ?

0

-

2.4.1.10

sucrose + sucrose = 6-kestose + levanbiose + blastose + 1-kestose + ?

0

-

2.4.1.10

sucrose + sucrose = D-glucose + 1-kestose

0

-

2.4.1.10

sucrose + sucrose = D-glucose + 1-kestose + nystose + ?

0

-

2.4.1.10

sucrose + glucose = 1-kestotriose + 6-kestotriose + 1-kestotetraose + ?

0

-

2.4.1.10

sucrose + raffinose = Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ?

0

-

2.4.1.10

jaggary = D-glucose + fructose + kestose + nystose + difructose + levan

0

-

2.4.1.10

sucrose + sucrose = kestose + D-glucose

0

-

2.4.1.10

sucrose = levan + kestose

0

-

2.4.1.10

surgarcane molasse = D-glucose + fructose + kestose + nystose + difructose + levan

0

-

2.4.1.10

sweet sorghum juice = D-glucose + fructose + kestose + nystose + difructose + levan

0

-

2.4.1.10

table sugar = D-glucose + fructose + kestose + nystose + difructose + levan

0

-

2.4.1.100

1,1-nystose + 1,1-nystose = sucrose + 1-kestose + oligofructan DP5 + oligofructan DP6

488355

-

2.4.1.100

sucrose + 1-kestose = 1-kestose + 6G-kestose + tetrasaccharide

488350

-

2.4.1.100

2 sucrose = D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside

0

-

2.4.1.243

1F,6G-di-beta-D-fructofuranosylsucrose + sucrose = 1-kestose + 1-kestose

0

-

2.4.1.243

neokestose + sucrose = 1-kestose + ?

0

-

2.4.1.243

sucrose + sucrose = 1-kestotriose + ?

0

-

2.4.1.9

sucrose + raffinose = glucose + 1-kestose

0

-

2.4.1.9

sucrose + sucrose = 1-kestose + nystose + fructooligosaccharides

0

-

2.4.1.9

sucrose + sucrose = kestose + nystose + fructooligosaccharides

0

-

2.4.1.99

1,1-nystose + 1,1-nystose = 1,1,1-fructosylnystose + 1-kestose

489636

-

2.4.1.99

crystalline cane sugar = 1-kestose + sucrose + D-glucose + nystose

0

-

2.4.1.99

sucrose + sucrose = 1-kestose + D-glucose

0

-

2.4.1.99

sucrose + sucrose = 1-kestose + nystose

0

-

2.4.1.99

sucrose + sucrose = 1-kestose + nystose + fructosylnystose

0

-

2.4.1.99

sucrose + sucrose = D-glucose + 1-kestose

0

-

2.4.1.99

sucrose + sucrose = D-glucose + 1F-beta-D-fructosylsucrose

0, 489628, 489629, 489636, 489630, 489632, 489649, 488358, 489635, 489634, 489639

-

2.4.1.99

2 sucrose = D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside

0

-

2.4.1.10

12 entries

2.4.1.100

3 entries

2.4.1.243

3 entries

2.4.1.9

3 entries

2.4.1.99

8 entries

3.2.1.154

1,6G-kestotetraose + H2O = 1-kestotriose + fructose

0

-

3.2.1.26

2 sucrose = 1-kestose + glucose

136125

-

3.2.1.64

1-fructosylnystose + H2O = beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose + nystose

136755

-

3.2.1.64

nystose + H2O = beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose

136755

-

3.2.1.7

inulin + H2O = 1,1,1-kestopentaose + 1-kestose

0

-

3.2.1.7

inulin + H2O = 1-kestose + ?

0

-

3.2.1.64

2 entries

3.2.1.7

2 entries

3.2.1.80

inulin + H2O = 1-kestose + ?

0

-

4.2.2.17

beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside = di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose

0

-

4.2.2.17

fructofuranosyl nystose = di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose

0

-

4.2.2.17

inulin = di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose

0

-

4.2.2.17

inulin = di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose

0

-

4.2.2.17

4 entries

4.2.2.18

beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside = di-D-fructofuranose 1,2':2,3' dianhydride + 1-kestose

0

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (6 results)

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Show Turnover Number (6 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.153

-999

-

5 mM substrate: wild-type enzyme about 23 mol fructose/mol enzyme, mutant about 8 mol fructose/mol enzyme

682147

3.2.1.26

1.5

-

alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose

695761

3.2.1.26

26.3

-

in 0.2M sodium acetate buffer (pH 5.6), at 50°C

680989

3.2.1.26

64

-

pH 5.0, 37°C

714490

3.2.1.26

107

-

pH 5.7, 37°C

708103

3.2.1.26

4 entries

3.2.1.80

21

-

in 50 mM sodium acetate buffer, pH 5.0, at 30°C

682426

K_M Value (16 results)

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Show KM Value (16 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.100

18

-

pH 5.5, 30°C

660021

2.4.1.100

119

-

-

488355

2.4.1.100

233

-

wild type enzyme, in 50 mM sodium acetate buffer pH 5.0, at 30°C

704883

2.4.1.100

377

-

mutant enzyme D281Y, in 50 mM sodium acetate buffer pH 5.0, at 30°C

704883

2.4.1.100

4 entries

2.4.1.243

88

-

pH 5.7, 30°C

660021

3.2.1.153

7

-

isoenzyme1-FeH w1

657048

3.2.1.153

7

-

isoenzyme1-FeH w2

657048

3.2.1.153

58

-

-

208825

3.2.1.26

1.3

-

in 0.2M sodium acetate buffer (pH 5.6), at 50°C

680989

3.2.1.26

1.7

-

pH 5.7, 37°C

708103

3.2.1.26

4.5

-

pH 5.5, 42°C, alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose

695761

3.2.1.26

49

-

pH 5.0, 37°C

714490

3.2.1.26

700

-

in 50 mM sodium acetate buffer (pH 5.0), at 30°C

680952

3.2.1.153

3 entries

3.2.1.26

5 entries

3.2.1.7

8.4

-

-

136822

3.2.1.80

0.6

-

in 50 mM sodium acetate buffer, pH 5.0, at 30°C

682426

3.2.1.80

7

-

pH 5.0, 30°C

657048

3.2.1.80

2 entries

References & Links

Literature References (82)

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Show Reference (82 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

136062

7764333

Purification and characterization of beta-fructofuranosidase from Aspergillus japonicus TIT-KJ1

1993

Duan, K.J.; Sheu, D.C.; Chen, J.S.

Biosci. Biotechnol. Biochem.

57

1811-1815

136125

8310063

Purification and characterization of three soluble invertases from barley (Hordeum vulgare L.) leaves

1993

Obenland, D.M.; Simmen, U.; Boller, T.; Wiemken, A.

Plant Physiol.

101

1331-1339

136755

10618232

Purification and characterization of 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2

2000

Saito, K.; Kondo, K.; Kojima, I.; Yokota, A.; Tomita, F.

Appl. Environ. Microbiol.

66

252-256

136822

-

Purification and substrate specificity of endo-type inulinase from Penicillium purpurogenum

1988

Onodera, S.; Shiomi, N.

Agric. Biol. Chem.

52

2569-2576

208824

-

Characterization of a fructan exohydrolase purified from barley stems that hydrolyzes multiple fructofuranosidic linkages

1998

Henson, C.A.; Livingston III, D.P.

Plant Physiol. Biochem.

36

715-720

208825

-

Purification and properties of a second fructan exohydrolase from the roots of Chicorium intybus

1999

De Roover, J.; Van Laere, A.; De Winter, M.; Timmermanns, J.W.; Van den Ende, W.

Physiol. Plant.

106

28-34

488346

-

Sucrose:sucrose fructosyltransferase and fructan:fructan fructosyltransferase from Allium cepa

1980

Henry, R.J.; Darbyshire, B.

Phytochemistry

19

1017-1020

488347

-

Purification and characterization of fructan:fructan fructosyltransferase from Jerusalem artichoke (Helianthus tuberosus K.)

1993

Luescher, M.; Frehner, M.; Noesberger, J.

New Phytol.

123

717-724

488349

16652946

Purification and characterization of wheat beta(2->1) fructan:fructan fructosyl transferase activity

1992

Jeong, B.R.; Housley, T.L.

Plant Physiol.

100

199-204

488350

100201941

A fructan: fructan fructosyltransferase activity from Lolium rigidum

1997

St.John, J.A.; Bonnett, G.D.; Simpson, R.J.; Tanner, G.J.

New Phytol.

135

235-247

488351

100201940

Identification of products formed by a fructan:fructan fructosyltransferase activity from Lolium rigidum

1997

St.John, J.A.; Sims, I.M.; Bonnett, G.D.; Simpson, R.J.

New Phytol.

135

249-257

488355

-

Purification and characterization of fructan: fructan fructosyl transferase from chicory roots

1996

Van den Ende, W.; Van Wonterghem, D.; Verhaert, P.; Dewil, E.; Van Laere, A.

Planta

199

493-502

488358

8641462

Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus

1996

Luescher, M.; Erdin, C.; Sprenger, N.; Hochstrasser, U.; Boller, T.; Wiemken, A.

FEBS Lett.

385

39-42

489628

-

Purification and characterization of sucrose:sucrose 1-fructosyltransferase from the roots of Asparagus (Asparagus officinalis L.)

1980

Shiomi, N.; Izawa, M.

Agric. Biol. Chem.

44

603-614

489629

-

Isolation and characterization of sucrose:sucrose 1F-beta-D-fructosyltransferase from tubers of Helianthus tuberosus

1990

Praznik, W.; Beck, R.H.F.; Spies, T.

Agric. Biol. Chem.

54

2429-2431

489630

-

Purification and properties of sucrose:sucrose 1F-beta-D-fructosyltransferase in onion seeds

1985

Shiomi, N.; Kido, H.; Kiriyama, S.

Phytochemistry

24

695-698

489632

-

Substrate specificity of fructosyl transferase from chicory roots

1971

Singh, R.; Bhatia, I.S.

Phytochemistry

10

2037-2039

489634

11080298

Cloning and functional analysis of sucrose:sucrose 1-fructosyltransferase from tall fescue

2000

Luscher, M.; Hochstrasser, U.; Vogel, G.; Aeschbacher, R.; Galati, V.; Nelson, C.J.; Boller, T.; Wiemken, A.

Plant Physiol.

124

1217-1227

489635

12231699

Fructan synthesis in excised barley leaves. Identification of two sucrose-sucrose fructosyltransferases induced by light and their separation from constitutive invertases

1993

Simmen, U.; Obenland, D.; Boller, T.; Wiemken, A.

Plant Physiol.

101

459-468

489636

12226250

Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus Colombia. II. Purification of sucrose:sucrose 1-fructosyltransferase and reconstitution of fructan synthesis in vitro with purified sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1-fructosyltransferase

1996

Koops, A.J.; Jonker, H.H.

Plant Physiol.

110

1167-1175

489639

9418047

Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom disks

1997

Hellwege, E.M.; Gritscher, D.; Willmitzer, L.; Heyer, A.G.

Plant J.

12

1057-1065

489649

1010572

Biosynthesis of oligosaccharides and fructans in Agave vera cruz: I. Properties of a partially purified transfructosylase

1976

Satyanarayana, M.N.

Indian J. Biochem. Biophys.

13

261-266

651408

12095678

Cloning and characterization of a levanbiohydrolase from Microbacterium laevaniformans ATCC 15953

2002

Song, E.K.; Kim, H.; Sung, H.K.; Cha, J.

Gene

291

45-55

655294

12029520

Molecular cloning and expression in Escherichia coli of an exo-levanase gene from the endophytic bacterium Gluconacetobacter diazotrophicus SRT4

2002

Menendez, C.; Hernandez, L.; Selman, G.; Mendoza, M.F.; Hevia, P.; Sotolongo, M.; Arrieta, J.G.

Curr. Microbiol.

45

5-12

657006

14617070

Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.)

2003

Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.

Plant J.

36

697-710

657048

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