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Ligand 5,6,7,8-tetrahydropteroyl-Glu4

Basic Ligand Information

Show all BRENDA pathways known for 5,6,7,8-tetrahydropteroyl-Glu4

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.5.3.24

sarcosine + tetrahydropteroyltetraglutamate + O2 = ?

764121

-

2.1.2.10

S-aminomethyldihydrolipoylprotein + tetrahydropteroyltetraglutamate = dihydrolipoylprotein + 5,10-methylenetetrahydropteroyltetraglutamate + NH3

485698

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2.1.2.11

tetrahydropteroyltetraglutamate + 2-dehydropantoate = 5,10-methylenetetrahydropteroyltetraglutamate + 3-methyl-2-oxobutanoate + H2O

485700

-

6.3.2.17

ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu5

1061

-

6.3.2.17

ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu5

1063

-

6.3.2.17

2 entries

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

6.3.2.17

ATP + 5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu3

649464, 653725

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6.3.2.17

ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu4

0

-

6.3.2.17

ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4

0

-

6.3.2.17

3 entries

Enzyme Kinetic Parameters

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.1.2.11

0.1

-

-

485700

6.3.2.17

0.0016

-

-

1089

6.3.2.17

0.002

-

-

1061, 1063

6.3.2.17

0.002

-

5,6,7,8-tetrahydropteroyl-Glu

1091

6.3.2.17

0.002

-

7,8-dihydropteroyl-Glu

1100

6.3.2.17

4 entries

References & Links

Literature References (10)

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Show Reference (10 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

1061

3828321

Mammalian folylpoly-gamma-glutamate synthetase. 2. Substrate specificity and kinetic properties

1987

Cichowicz, D.J.; Shane, B.

Biochemistry

26

513-521

1063

-

Folylpoly-gamma-glutamate synthetase

1986

Shane, B.

Chem. Biol. Pteridines, Proc. Int. Symp. Pteridines Folic Acid Deriv. Chem. Biol. Clin. Aspects

-

719-728

1089

9250977

Human cytosolic folylpoly-gamma-glutamate synthase

1997

Atkinson, I.; Garrow, T.; Brenner, A.; Shane, B.

Methods Enzymol.

281

134-140

1091

8304201

Purification and general properties of human folylpolyglutamate synthetase

1993

Garrow, T.A.; Shane, B.

Chem. Biol. Pteridines Folates (J. E. Ayling et al eds. ) Plenum Press New York

-

659-662

1100

7646057

DL-beta,beta-Difluoroglutamic acid mediates position-dependent enhancement or termination of pteroylpoly(glutamate)synthesis catalyzed by folylpolyglutamate synthetase

1995

McGuire, J.J.; Hart, B.P.; Haile, W.H.; Rhee, M.S.; Galivan, J.; Coward, J.K.

Arch. Biochem. Biophys.

321

319-328

485698

10364177

Identification of the folate binding sites on the Escherichia coli T-protein of the glycine cleavage system

1999

Okamura-Ikeda, K.; Fujiwara, K.; Motokawa, Y.

J. Biol. Chem.

274

17471-17477

485700

6463

Ketopantoate hydroxymethyltransferase. II. Physical, catalytic, and regulatory properties

1976

Powers, S.G.; Snell, E.E.

J. Biol. Chem.

251

3786-3793

649464

12051687

Mutation of an essential glutamate residue in folylpolyglutamate synthetase and activation of the enzyme by pteroate binding

2002

Sheng, Y.; Cross, J.A.; Shen, Y.; Smith, C.A.; Bognar, A.L.

Arch. Biochem. Biophys.

402

94-103

653725

10648167

Purification and characteristics of recombinant human folylpoly-gamma-glutamate synthetase expressed at high levels in insect cells

2000

Sanghani, P.C.; Moran, R.G.

Protein Expr. Purif.

18

36-45

764121

3427080

Interaction of tetrahydrofolate and other folate derivatives with bacterial sarcosine oxidase

1987

Kvalnes-Krick, K.; Jorns, M.S.

Biochemistry

26

7391-7395

Links to other databases for 5,6,7,8-tetrahydropteroyl-Glu4

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Show Links (1 entry)

ChEBI

PubChem

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