Ligand MgATP2-

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Basic Ligand Information

Molecular Structure
Picture of MgATP2- (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C10H16MgN5O13P3
MgATP2-
CYYJCOXYBYJLIK-MTLYETOXSA-J
Synonyms:
Mg-ATP, Mg-ATP-complex, Mg-ATP2-, MgATP

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
6-hydroxymethyl-7,8-dihydropterin + MgATP2- = 6-hydroxymethyl-7,8-dihydropterin diphosphate + MgAMP
show the reaction diagram
-
MgATP2- = ?
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (21 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
luciferin + MgATP2- = AMP + diphosphate
show the reaction diagram
-
MgATP2- + beta-D-fructose 6-phosphate = ?
show the reaction diagram
-
MgATP2- + UMP = MgADP- + UDP
show the reaction diagram
-
GMP + MgATP2- = MgADP- + GDP
show the reaction diagram
-
Mg-ATP2- + D-ribose 5-phosphate = Mg-AMP2- + 5-phospho-alpha-D-ribose 1-diphosphate
show the reaction diagram
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6-hydroxymethyl-7,8-dihydropterin + MgATP2- = 6-hydroxymethyl-7,8-dihydropterin diphosphate + MgAMP
show the reaction diagram
-
MgATP2- + H2O = ?
show the reaction diagram
-
MgATP2- = ?
show the reaction diagram
-
gamma-L-Glu-L-Cys-Gly + spermidine + MgATP2- = N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + MgADP- + phosphate
show the reaction diagram
-
XMP + Mg-ATP2- = adenyl-XMP + diphosphate + Mg2+
show the reaction diagram
-
MgATP + H2O = MgADP + phosphate
show the reaction diagram
-

Enzyme Cofactor/Cosubstrate (9 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
required
-
preferred substrate
-
enzyme is inhibited by salt, therefore MgATP2- is used as cofactor instead of MgCl2 and NaATP-
-
neccasary for the pump function
-

Activator in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
conversation of 2,4-dichlorobenzoyl-CoA in cytosolic fraction
-
the apparent Km for MgATP2- is 0.022 mM
-
activation, about 15% as efficient as AMP
-
C-trehalase can be activated by MgATP2- in presence of cAMP
-
activation of H+-ATPase occurs by the addition of 5 mM Mg-ATP
-

Inhibitor in Enzyme-catalyzed Reactions (48 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
due to phosphorylation of the enzyme by pyruvate dehydrogenase kinase
-
extreme inhibition in all microsomal fractions
-
32% and 54% inhibition at 0.125 and 1 mM respectively in neuronal nuclear fraction
-
reversible by AMP
-
above 1 mM
-
substrate inhibition
-
enzyme form AK2 is strongly inhibited at high concentrations
-
competitive with UMP
-
metal chelates being the true substrates, free nucleotides being inhibitors
-
inhibits SMMP activity towards P-myosin, disassembles P-myosin mostly into a folded conformation
-
inhibits the enzyme slightly
-
product inhibition
-
substrate inhibition
-

Metals and Ions (8 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (20 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (111 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.3
-
ATP in form of MgATP2-
0.014
-
pH 7.2, 37ºC
0.22
-
forward reaction
0.0185
-
concentration of MgATP2- higher than 0.006 mM
0.25
-
-
0.62
-
-
0.15
-
pH 7.5, 37°C
0.17
-
-
0.97
-
-
0.018
-
ATP
0.04
-
-
0.11
-
-
0.22
-
pH 7.5, 37°C, ATPase activity of N-terminal cytoplasmic domain

Ki Value (22 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.864
-
pH 9.0, 25°C

References & Links

Links to other databases for MgATP2-

ChEBI
PubChem
-
PubChem