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Ligand ganglioside GD1b

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (6 results)

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Show Substrate (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.45

acetyl-CoA + ganglioside GD1b = ?

758502

-

2.4.3.2

CMP-N-acetyl-beta-neuraminate + ganglioside GD1b = CMP + ganglioside GT1b

637737, 637742, 744832, 744836, 637741, 745233

-

3.2.1.123

ganglioside GD1b + H2O = ?

698135, 732295

-

3.2.1.18

ganglioside GD1b + H2O = ?

171117, 171152, 665540, 666001, 171122, 171151, 171164, 729808

-

3.2.1.18

ganglioside GD1b + H2O = sialic acid + ?

655256

-

3.2.1.18

2 entries

3.5.1.69

ganglioside GD1b + H2O = fatty acid + ?

652249

-

Enzyme Kinetic Parameters

K_M Value (6 results)

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Show KM Value (6 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.3.2

0.000196

-

-

637742

2.4.3.2

0.15

-

pH 6.0, 30°C

744832

3.2.1.18

0.1

-

-

171152

3.2.1.18

0.13

-

-

171151

3.2.1.18

0.51

-

37°C, pH 5.6 or 7.0 activated by 0.02% Triton X-100

665540

3.2.1.18

0.59

-

37°C, pH 5.6 or 7.0, without Triton X-100

665540

2.4.3.2

2 entries

3.2.1.18

4 entries

References & Links

Literature References (19)

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Show Reference (19 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

171117

3654592

Human placental sialidase: partial purification and characterization

1987

Hiraiwa, M.; Uda, Y.; Nishizawa, M.; Miyatake, T.

J. Biochem.

101

1273-1279

171122

3997846

Purification and characterization of cytosolic sialidase from rat liver

1986

Miyagi, T.; Tsuiki, S.

J. Biol. Chem.

260

6710-6716

171151

4858814

Studies on brain membrane-bound neuraminidase

1974

Preti, A.; Lombardo, A.; Cestaro, B.; Zambotti, S.; Tettamanti, G.

Biochim. Biophys. Acta

350

406-414

171152

4711815

The purification and properties of a mammalian neuraminidase (sialidase)

1973

Tallman J.F.; Brady, R.O.

Biochim. Biophys. Acta

293

434-443

171164

8174546

Sialidase in rabbit blood

1994

Chen, X.G.; Nagai, T.; Yamada, H.

Eur. J. Biochem.

221

655-664

637737

1991463

Substrate specificity of alpha 2->3-sialyltransferases in ganglioside biosynthesis of rat liver Golgi

1991

Iber, H.; van Echten, G.; Sandhoff, K.

Eur. J. Biochem.

195

115-120

637741

2262006

Purification and characterization of CMP-NeuAc:GM1 (Galbeta1-4GalNAc) alpha 2-3 sialyltransferase from rat brain

1990

Gu, T.J.; Gu, X.B.; Ariga, T.; Yu, R.K.

FEBS Lett.

275

83-86

637742

2120225

Sub-Golgi distribution in rat liver of CMP-NeuAc GM3- and CMP-NeuAc:GT1b alpha 2->8sialyltransferases and comparison with the distribution of the other glycosyltransferase activities involved in ganglioside biosynthesis

1990

Trinchera, M.; Pirovano, B.; Ghidoni, R.

J. Biol. Chem.

265

18242-18247

652249

11827965

Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8

2002

Furusato, M.; Sueyoshi, N.; Mitsutake, S.; Sakaguchi, K.; Kita, K.; Okino, N.; Ichinose, S.; Omori, A.; Ito, M.

J. Biol. Chem.

277

17300-17307

655256

11026668

Enzymatic properties of sialidase from the ovary of the starfish, Asterina pectinifera

2000

Iriyama, N.; Takeuchi, N.; Shiraishi, T.; Izumi, K.; Sawada, M.T.; Takahashi, N.; Furuhata, K.; Ogura, H.; Uda, Y.

Comp. Biochem. Physiol. B

126

561-569

665540

14613940

Properties of recombinant human cytosolic sialidase HsNEU2: The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules

2004

Tringali, C.; Papini, N.; Fusi, P.; Croci, G.; Borsani, G.; Preti, A.; Tortora, P.; Tettamanti, G.; Venerando, B.; Monti, E.

J. Biol. Chem.

279

3169-3179

666001

-

Cloning of human liver cytosolic sialidase from genomic DNA using splicing by overlap extension and its characterization

2005

Ha, K.; Cho, S.; Kang, S.; Kim, Y.; Kim, J.; Kim, C.

J. Microbiol. Biotechnol.

15

722-727

698135

18415015

Preparation of homogenous oligosaccharide chains from glycosphingolipids

2009

Li, Y.T.; Chou, C.W.; Li, S.C.; Kobayashi, U.; Ishibashi, Y.H.; Ito, M.

Glycoconj. J.

26

929-933

729808

25294388

Mapping substrate interactions of the human membrane-associated neuraminidase, NEU3, using STD NMR

2015

Albohy, A.; Richards, M.R.; Cairo, C.W.

Glycobiology

25

284-293

732295

22798689

Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system

2012

Ishibashi, Y.; Kobayashi, U.; Hijikata, A.; Sakaguchi, K.; Goda, H.M.; Tamura, T.; Okino, N.; Ito, M.

J. Lipid Res.

53

2242-2251

744832

8375377

Molecular cloning and expression of Galbeta1,3GalNAcalpha2,3-sialyltransferase from mouse brain

1993

Lee, Y.C.; Kurosawa, N.; Hamamoto, T.; Nakaoka, T.; Tsuji, S.

Eur. J. Biochem.

216

377-385

744836

9266697

Cloning and expression of cDNA for a human Gal(beta1-3)GalNAc alpha2,3-sialyltransferase from the CEM T-cell line

1997

Giordanengo, V.; Bannwarth, S.; Laffont, C.; Van Miegem, V.; Harduin-Lepers, A.; Delannoy, P.; Lefebvre, J.C.

Eur. J. Biochem.

247

558-566

745233

17673486

Molecular cloning, expression and properties of an alpha/beta-galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16

2007

Takakura, Y.; Tsukamoto, H.; Yamamoto, T.

J. Biochem.

142

403-412

758502

31202640

Neurostatin and other O-acetylated gangliosides show anti-neuroinflammatory activity involving the NFkappaB pathway

2019

Yanguas-Casas, N.; Ojalvo-Sanz, A.C.; Martinez-Vazquez, A.; Goneau, M.F.; Gilbert, M.; Nieto-Sampedro, M.; Romero-Ramirez, L.

Toxicol. Appl. Pharmacol.

377

114627

Links to other databases for ganglioside GD1b

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ChEBI

PubChem

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