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Ligand gamma-Glu-2-aminobutyrate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (6 results)

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Show Substrate (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.3.2.9

5-(4-methyl)glutamyl-L-2-aminobutyrate = 3-methyl-5-oxoproline + L-alpha-aminobutyrate

487983

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4.3.2.9

5-L-glutamyl-L-alpha-aminobutyrate = 5-L-oxoproline + L-alpha-aminobutyrate

487979, 487981, 487982, 487975, 487983, 487978, 487980, 487984

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6.3.2.3

ATP + gamma-Glu-2-aminobutyrate + hydroxylamine = ADP + phosphate + gamma-(alpha-aminomethyl)Glu-2-aminobutyryl-Gly

1197

-

6.3.2.3

ATP + gamma-Glu-aminobutyrate + Gly = ADP + phosphate + ophthalmic acid

1197, 1223, 1190, 1185, 1194, 1191

-

6.3.2.3

ATP + gamma-L-Glu-L-alpha-aminobutyrate + Gly = ADP + phosphate + glutathione

649553

-

6.3.2.3

ATP + L-gamma-glutamyl-L-alpha-aminobutyrate + glycine = ADP + L-gamma-glutamyl-L-alpha-aminobutyryl-glycine

746387

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4.3.2.9

2 entries

6.3.2.3

4 entries

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.2

5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate = 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine

0

-

6.3.2.2

ATP + L-Glu + L-2-aminobutanoate = ADP + phosphate + gamma-L-Glu-2-aminobutanoate

0, 1126

-

6.3.2.2

ATP + L-Glu + L-alpha-aminobutyrate = ADP + phosphate + gamma-L-Glu-L-alpha-aminobutyrate

650713, 649263, 651896, 652192, 650135, 652341

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6.3.2.2

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

6.3.2.3

13

-

recombinant enzyme, pH 8.2, 37°C

649553

K_M Value (9 results)

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Show KM Value (9 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

4.3.2.9

6

-

isoenzyme with pI 5.1, in extract, Km increases 4-10fold during purification

487979

4.3.2.9

6.6

-

isoenzyme with pI 4.6

487979

4.3.2.9

8

-

isoenzyme B

487978

4.3.2.9

12

-

isoenzyme A

487978

6.3.2.3

0.042

-

recombinant enzyme, pH 8.2, 37°C

649553

6.3.2.3

0.091

-

-

1190

6.3.2.3

0.22

-

-

1185

6.3.2.3

0.5

-

-

1197, 1199

6.3.2.3

0.5

-

ATP

1197

4.3.2.9

4 entries

6.3.2.3

5 entries

References & Links

Literature References (24)

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Show Reference (24 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

1126

-

gamma-Glutamylcysteine synthetase from Proteus mirabilis

1982

Kumagai, H.; Nakayama, R.; Tochikura, T.

Agric. Biol. Chem.

46

1301-1309

1185

-

Homoglutathione and glutathione synthetases of legume seedlings: partial purification and substrate specificity

1987

Macnicol, P.K.

Plant Sci.

53

229-235

1190

-

Purification and properties of glutathione synthetase from spinach (Spinacia oleracea) leaves

1986

Law, M.Y.; Halliwell B.

Plant Sci.

43

185-191

1191

3911005

Glutathione synthetase from rat kidney

1985

Meister, A.

Methods Enzymol.

113

393-399

1194

447639

Glutathione synthetase. Purification from rat kidney and mapping of the substrate binding sites

1979

Oppenheimer, L.; Wellner, V.P.; Griffith, O.W.; Meister.A.

J. Biol. Chem.

254

5184-5190

1197

-

Glutathione synthesis

1974

Meister, A.

The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )

10

671-697

1199

-

Glutathione biosynthesis. I. gamma-Glutamylcysteine synthetase (hog liver). II. Glutathione (tripeptide) synthetase (yeast)

1971

Mooz, E.D.; Meister, A.

Methods Enzymol.

17B

483-495

1223

5066151

Kinetische Versuche zum Reaktionsmechanismus der Glutathion-synthetase (EC 6.3.2.3)

1972

Wendel, A.; Flohe, L.

Hoppe-Seyler's Z. Physiol. Chem.

353

523-530

487975

5781001

Isolation and properties of gamma-L-glutamylcyclotransferase from human brain

1969

Orlowski, M.; Richman, P.G.; Meister, A.

Biochemistry

8

1048-1055

487978

4735346

gamma-Glutamyl cyclotransferase. Distribution, isozymic forms, and specificity

1973

Orlowski, M.; Meister, A.

J. Biol. Chem.

248

2836-2844

487979

-

gamma-Glutamylcyclotransferase from rat kidney

1981

Meister, A.

Methods Enzymol.

113

428-445

487980

24630

gamma-Glutamyl cyclotransferase from rat kidney. Sulfhydryl groups and isolation of a stable form of the enzyme

1978

Taniguchi, N.; Meister, A.

J. Biol. Chem.

253

1799-1806

487981

6107299

L-gamma-(threo-beta-methyl)glutamyl-L-alpha-aminobutyrate, a selective substrate of alpha-glutamyl cyclotransferase

1980

Bridges, R.J.; Griffith, O.W.; Meister, A.

J. Biol. Chem.

255

10787-10792

487982

-

Enzymology of pyrrolidone carboxylic acid

1971

Orlowski, M.; Meister, A.

The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )

4

123-151

487983

237623

Specificity of gamma-glutamyl cyclotransferase

1975

Szweczuk, A.; Connell, G.E.

Can. J. Biochem.

53

706-712

487984

20626

Selective inhibition of gamma-glutamyl-cycle enzymes by substrate analogs

1977

Griffith, O.W.; Meister, A.

Proc. Natl. Acad. Sci. USA

74

3330-3334

649263

10218110

The enzymes of glutathione synthesis: gamma-glutamylcysteine synthetase

1999

Griffith, O.W.; Mulcahy, R.T.

Adv. Enzymol. Relat. Areas Mol. Biol.

73

209-267

649553

10964706

Novel kinetics of mammalian glutathione synthetase: characterization of gamma-glutamyl substrate cooperative binding

2000

Luo, J.L.; Huang, C.S.; Babaoglu, K.; Anderson, M.E.

Biochem. Biophys. Res. Commun.

275

577-581

650135

11851421

Substrate binding determinants of Trypanosoma brucei gamma-glutamylcysteine synthetase

2002

Abbott, J.J.; Ford, J.L.; Phillips, M.A.

Biochemistry

41

2741-2750

650713

12663448

A novel missense mutation in the gamma-glutamylcysteine synthetase catalytic subunit gene causes both decreased enzymatic activity and glutathione production

2003

Hamilton, D.; Wu, J.H.; Alaoui-Jamali, M.; Batist, G.

Blood

102

725-730

651896

9756861

Trypanosoma brucei gamma-glutamylcysteine synthetase. Characterization of the kinetic mechanism and the role of Cys-319 in cystamine inactivation

1998

Brekken, D.L.; Phillips, M.A.

J. Biol. Chem.

273

26317-26322

652192

11527962

Structure prediction and active site analysis of the metal binding determinants in gamma-glutamylcysteine synthetase

2001

Abbott, J.J.; Pei, J.; Ford, J.L.; Qi, Y.; Grishin, V.N.; Pitcher, L.A.; Phillips, M.A.; Grishin, N.V.

J. Biol. Chem.

276

42099-42107

652341

11675389

Escherichia coli gamma-glutamylcysteine synthetase. Two active site metal ions affect substrate and inhibitor binding

2002

Kelly, B.S.; Antholine, W.E.; Griffith, O.W.

J. Biol. Chem.

277

50-58

746387

25070563

The role of strong electrostatic interactions at the dimer interface of human glutathione synthetase

2014

De Jesus, M.C.; Ingle, B.L.; Barakat, K.A.; Shrestha, B.; Slavens, K.D.; Cundari, T.R.; Anderson, M.E.

Protein J.

33

403-409

Links to other databases for gamma-Glu-2-aminobutyrate

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ChEBI

PubChem

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