print visible entries

print all entries

Ligand 5beta-Pregnan-3alpha,21-diol-20-one

Basic Ligand Information

Molecular Structure

close all tables

open all tables

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (3 results)

top print hide

Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.149

5beta-pregnan-3alpha,21-diol-20-one + NADPH + H+ = ?

740126

-

1.1.1.150

5beta-pregnane-3alpha,21-diol-20-one + NAD+ = 5beta-pregnane-3alpha-ol-20,21-dione + NADH + H+

286007

-

1.1.1.239

5beta-pregnan-3alpha,21-diol-20-one + NAD+ = 5beta-pregnan-21-ol-3,20-dione + NADH

286354

-

Product in Enzyme-catalyzed Reactions (1 result)

top print hide

Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.209

5alpha-pregnane-21-ol-3,20-dione + NADPH = 5beta-pregnane-3alpha,21-diol-20-one + NADP+

286234

-

Inhibitor in Enzyme-catalyzed Reactions (2 results)

top print hide

Show Inhibitors (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.146

IC50: 0.003 mM

285948

-

1.1.1.B40

not inhibitory

285948

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

top print hide

Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.149

0.017

-

at pH 7.4 and 37°C

740126

1.1.1.213

0.633

-

-

286354

1.1.1.239

0.367

-

-

286354

K_M Value (1 result)

top print hide

Show KM Value (1 entry)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.149

0.0026

-

at pH 7.4 and 37°C

740126

IC₅₀ Value (1 result)

top print hide

Show IC50 Value (1 entry)

EC NUMBER

IC50 VALUE

IC50 VALUE MAXIMUM

COMMENTARY

LITERATURE

1.1.1.146

0.003

-

IC50: 0.003 mM

285948

References & Links

Literature References (6)

top print hide

Show Reference (6 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

285948

9055382

Selective inhibition of sheep kidney 11beta-hydroxysteroid dehydrogenase isoform 2 activity by 5alpha-reduced (but not 5beta) derivates of adrenocorticosteroids

1997

Latif, S.A.; Sheff, M.F.; Ribeiro, C.E.; Morris, D.J.

Steroids

62

230-237

286007

-

Stereochemistry of hydrogen loss during C-21 dehydroxylation of tetrahydrodeoxycorticosterone by Eubacterium lentum

1989

Holland, H.L.; Ninniss, R.W.; Brown, F.M.

Can. J. Chem.

67

1590-1595

286234

2691507

Identification of two dihydrodiol dehydrogenases associated with 3(17)alpha-hydoxysteroid dehydrogenase activity in mouse kidney

1989

Nakagawa, M.; Tsukuda, F.; Nakayama, T.; Matsuura, K.; Hara, A.; Sawada, H.

J. Biochem.

106

633-638

286234

2691507

Identification of two dihydrodiol dehydrogenases associated with 3(17)alpha-hydroxysteroid dehydrogenase activity in mouse kidney

1989

Nakagawa, M.; Tsukada, F.; Nakayama, T.; Matsuura, K.; Hara, A.; Sawada, H.

J. Biochem.

106

633-638

286234

2 entries

286354

2317205

Demonstration of 3alpha(17beta)-hydroxysteroid dehydrogenase distinct from 3alpha-hydroxysteroid dehydrogenase in hamster liver

1990

Ohmura, M.; Hara, A.; Nakagawa, M.; Sawada, H.

Biochem. J.

266

583-589

740126

23995665

Substrate specificity and inhibitor sensitivity of rabbit 20alpha-hydroxysteroid dehydrogenase

2013

Endo, S.; Arai, Y.; Hara, A.; Kitade, Y.; Bunai, Y.; El-Kabbani, O.; Matsunaga, T.

Biol. Pharm. Bull.

36

1514-1518

Links to other databases for 5beta-Pregnan-3alpha,21-diol-20-one

top print hide

Show Links (1 entry)