Ligand pyridoxine

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Basic Ligand Information

Molecular Structure
Picture of pyridoxine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C8H11NO3
pyridoxine
LXNHXLLTXMVWPM-UHFFFAOYSA-N
Synonyms:
4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol, pyridoxine hydrochloride, pyridoxol


Show all pahtways known for Show all BRENDA pathways known for pyridoxine

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxine + NADP+ = pyridoxal + NADPH
show the reaction diagram
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pyridoxine + O2 = pyridoxal + H2O2
show the reaction diagram
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pyridoxine + acceptor = isopyridoxal + reduced acceptor
show the reaction diagram
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UDPglucose + pyridoxine = UDP + 5'-O-beta-D-glucosylpyridoxine
show the reaction diagram
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ATP + pyridoxine = ADP + pyridoxine 5'-phosphate
show the reaction diagram

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxine-5'-beta-D-glucoside + H2O = pyridoxine + D-glucose
show the reaction diagram
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-

Substrate in Enzyme-catalyzed Reactions (16 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxine + NADP+ = pyridoxal + NADPH
show the reaction diagram
-
UDPglucose + pyridoxine = UDP + 5'-O-beta-D-glucosylpyridoxine
show the reaction diagram
-
thiamine + pyridoxine = ?
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ATP + pyridoxine = ADP + pyridoxine 5'-phosphate
show the reaction diagram
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maltose + pyridoxine = ?
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Product in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxal + NADPH = 4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol + NADP+
show the reaction diagram
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pyridoxine + 2,6-dichloroindophenol = pyridoxol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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sucrose + D-arabitol = pyridoxine + alpha-D-glucose 1-phosphate
show the reaction diagram
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pyridoxine 5'-phosphate + H2O = pyridoxine + phosphate
show the reaction diagram
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pyridoxal phosphate + H2O = pyridoxol + phosphate
show the reaction diagram
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-
pyridoxine-5'-beta-D-glucoside + H2O = pyridoxine + D-glucose
show the reaction diagram
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-

Activator in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
0.1 mM, 1.5fold increase in activity of isozyme B
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stimulates activity in crude extracts from pyridoxine-deficient felts, no activation of purified enzyme
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20 mM, stimulation to 120% of the control
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Inhibitor in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
lower inhibitry potency, about 25% inhibition at 0.0025 mM
-
approx. 0.1 mM, 33% inhibition of isozyme C, 0.39 mM, 50% inhibition
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competitive inhibitor of pyridoxamine glucosylation
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substrate inhibition above 0.2 mM
inhibits competitively the phosphorylation of 2-methyl-4-amino-5-hydroxymethylpyrimidine
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3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (16 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
22
-
acceptor O2

KM Value (31 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.25
-
-

Ki Value (2 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.33
-
inhibition of isozyme C
0.0027
-
pH 6.2, 37°C

References & Links

Links to other databases for pyridoxine

ChEBI
PubChem
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PubChem