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Ligand 2,4-dinitrophenyl-beta-D-glucoside

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.21

2,4-dinitrophenyl beta-D-glucoside + H2O = ?

729287

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3.2.1.23

2,4-dinitrophenyl-beta-D-glucoside + H2O = 2,4-dinitrophenol + beta-D-glucose

171271

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (4 results)

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Show Turnover Number (4 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.21

0.34

-

mutant enzyme E490A, in 0.1 M sodium acetate, pH 6.0, at 40°C

729287

3.2.1.21

2.12

-

mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C

729287

3.2.1.21

71

-

wild type enzyme, in 0.1 M sodium acetate, pH 6.0, at 40°C

729287

3.2.1.21

422

-

wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C

729287

3.2.1.21

4 entries

K_M Value (4 results)

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Show KM Value (4 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.21

0.015

-

mutant enzyme E490A, in 0.1 M sodium acetate, pH 6.0, at 40°C

729287

3.2.1.21

0.1

-

mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C

729287

3.2.1.21

0.26

-

wild type enzyme, in 0.1 M sodium acetate, pH 6.0, at 40°C

729287

3.2.1.21

0.49

-

wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C

729287

3.2.1.21

4 entries

References & Links

Literature References (2)

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Show Reference (2 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

171271

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beta-Galactosidase

1972

Wallenfels, K.; Weil, R.

The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )

7

617-663

729287

23201198

Identification of the acid/base catalyst of a glycoside hydrolase family 3 (GH3) beta-glucosidase from Aspergillus niger ASKU28

2013

Thongpoo, P.; McKee, L.S.; Araujo, A.C.; Kongsaeree, P.T.; Brumer, H.

Biochim. Biophys. Acta

1830

2739-2749

Links to other databases for 2,4-dinitrophenyl-beta-D-glucoside

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Show Links (1 entry)

ChEBI

PubChem

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