Ligand RNA

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Basic Ligand Information

Molecular Structure
Picture of RNA (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C47H58N17O37P5R2
RNA
UQUGGTXTQMNFQI-ZPHKRAHOSA-N
Synonyms:
ribonucleic acid

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (26 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
RNA + H2O = 5'-phosphomononucleotides
show the reaction diagram
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RNA + H2O = ?
show the reaction diagram
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RNA + H2O = nucleoside 5'-monophosphates
show the reaction diagram
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RNA + H2O = oligonucleotides
show the reaction diagram
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RNA + H2O = ?
show the reaction diagram
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RNA + H2O = ?
show the reaction diagram
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RNA + H2O = ?
show the reaction diagram
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RNA + H2O = adenosine 5'-monophosphate + ?
show the reaction diagram
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RNA + H2O = ?
show the reaction diagram
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RNA + H2O = ?
show the reaction diagram
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RNA + H2O = nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
show the reaction diagram
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RNA + H2O = 2',3'-cyclic nucleotides
show the reaction diagram
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RNA + H2O = ?
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (70 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
GTP + RNA = ?
show the reaction diagram
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RNA + H2O = 5'-phosphomononucleotides
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = oligonucleotides
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
RNA + H2O = adenosine 5'-monophosphate + ?
show the reaction diagram
-
RNA + H2O = nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
show the reaction diagram
-
RNA + H2O = 2',3'-cyclic nucleotides
show the reaction diagram
-
RNA + H2O = ?
show the reaction diagram
-
ATP + RNA + 5'-aaCCAGUC = AMP + diphosphate + ?
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pre-tRNA precursor + H2O = mat-tRNA + RNA
show the reaction diagram
-
-

Activator in Enzyme-catalyzed Reactions (18 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
below 0.015 mg/ml activation, rat brain enzyme contains RNA, about 10% of the total mass
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RNA binding is integral to APOBEC3H function. RNA-mediated dimerization alters the interactions of the enzyme with ssDNA and other RNA molecules
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stimulates the ATP hydrolysis dependent on the chain length
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stimulates
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synthetic and mRNA-type RNAs stimulates activity efficiently
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stimulates exchange reaction between ATP and diphosphate
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Inhibitor in Enzyme-catalyzed Reactions (18 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
above 0.015 mg/ml
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the presence of RNA strongly inhibits enzyme activity in vitro
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inhibited by high non-template RNA concentrations. This inhibition indicates feedback regulation of minus-strand synthesis
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enhanced activity by pretreatment with ribonuclease, variety of RNA's including tRNA
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5S RNA or a mixture of 16S and 23S rRNA
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substrate inhibition above 120 µg/ml
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competitive
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calf liver RNA
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yeast RNA in presence of 0-50 mM NaCl
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homopolymeric RNA inhibits under otherwise optimal conditions
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strong inhibition
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0.01 mM
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (20 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.005
-
wild-type transcriptase cofactor my2, 22°C, 6.5-7.0
35.7
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pH 7.0, 37°C
0.15
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KM Value (30 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.00015
-
-
0.144
-
-
-999
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at the optimal Mg2+ amount, both the Km and the Kcat values are similar to both RNA and DNA substrates
0.00032
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wild-type transcriptase cofactor my2, 22°C, 6.5-7.0

References & Links

Links to other databases for RNA

ChEBI
PubChem
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PubChem