Ligand (S)-2-Hydroxy-2-methyl-3-oxobutanoate

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Basic Ligand Information

Molecular Structure
Picture of (S)-2-Hydroxy-2-methyl-3-oxobutanoate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C5H8O4
(S)-2-Hydroxy-2-methyl-3-oxobutanoate
NMDWGEGFJUBKLB-YFKPBYRVSA-N
Synonyms:
(2S)-2-hydroxy-2-methyl-3-oxobutanoate, (S)-2-acetolactate, (S)-2-ethyl-2-hydroxy-3-oxobutanoate, (S)-acetolactate


Show all pahtways known for Show all BRENDA pathways known for (S)-2-Hydroxy-2-methyl-3-oxobutanoate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
pyruvate = (S)-2-acetolactate + CO2
-

Substrate in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ = (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-

Product in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
pyruvate = (S)-acetolactate + CO2
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (7 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
at pH 8.5 and 37°C
1.4
-
mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C
1.3
-
mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C
3.81
-
wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C
1.09
-
wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C
0.77
-

KM Value (7 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
at pH 8.5 and 37°C
0.11
-
mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C
0.04
-
mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C
0.15
-
wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C
0.24
-
wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C
0.08
-
at pH 6.0 and 45°C
0.0177
-
pH 6
1.3
-

References & Links

Links to other databases for (S)-2-Hydroxy-2-methyl-3-oxobutanoate

ChEBI
PubChem
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PubChem