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Ligand Penicillin K

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.5.1.11

penicillin K + H2O = ?

711057

-

3.5.1.11

penicillin K + H2O = octanoic acid + 6-aminopenicillanate

733165

-

3.5.1.11

2 entries

Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.5.1.11

penicillin K + H2O = 6-aminopenicillanic acid + octanoic acid

684562

-

3.5.1.11

penicillin K + H2O = ?

711057

-

3.5.1.11

penicillin K + H2O = octanoic acid + 6-aminopenicillanate

733165, 752541

-

3.5.1.70

penicillin K + H2O = 6-aminopenicillanic acid + octanoic acid

711493

-

3.5.1.70

penicillin K + H2O = ?

684562

-

3.5.1.11

3 entries

3.5.1.70

2 entries

Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.5.1.11

6-aminopenicillanic acid + methyl n-octanoate = penicillin K + methanol

0

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (10 results)

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Show Turnover Number (10 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.5.1.11

0.32

-

pH 5.5, 65°C, recombinant mutant L24F

733165

3.5.1.11

0.36

-

pH 5.5, 65°C, recombinant mutant S189F

733165

3.5.1.11

0.43

-

pH 5.5, 65°C, recombinant mutant L188F

733165

3.5.1.11

0.75

-

pH 5.5, 65°C, recombinant mutant L24F/I57F

733165

3.5.1.11

1.43

-

pH 5.5, 65°C, recombinant mutant L188F/L24F/I57F

733165

3.5.1.11

4.91

-

pH 5.5, 65°C, recombinant mutant I57F

733165

3.5.1.11

5.6

-

pH 5.5, 65°C, recombinant wild-type enzyme

733165

3.5.1.11

33.3

-

pH 8.0, 45°C

684562

3.5.1.11

35.58

-

pH 8.0, 40°C recombinant enzyme

752541

3.5.1.11

9 entries

3.5.1.70

33.3

-

pH 8.0, 45°C

684562

K_M Value (10 results)

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Show KM Value (10 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.5.1.11

0.05

-

pH 5.5, 65°C, recombinant mutant L24F/I57F

733165

3.5.1.11

0.1

-

pH 5.5, 65°C, recombinant mutants L188F and S189F

733165

3.5.1.11

0.12

-

pH 5.5, 65°C, recombinant mutant L24F

733165

3.5.1.11

0.14

-

pH 8.0, 40°C recombinant enzyme

752541

3.5.1.11

0.15

-

pH 5.5, 65°C, recombinant mutant L188F/L24F/I57F

733165

3.5.1.11

0.27

-

pH 5.5, 65°C, recombinant mutant I57F

733165

3.5.1.11

0.32

-

pH 5.5, 65°C, recombinant wild-type enzyme

733165

3.5.1.11

1

-

pH 8.0, 45°C

684562

3.5.1.70

0.45

-

recombinant immobilized enzyme, Vmax: 7.6 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C

711493

3.5.1.70

1

-

pH 8.0, 45°C

684562

3.5.1.11

8 entries

3.5.1.70

2 entries

References & Links

Literature References (5)

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Show Reference (5 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

684562

17586674

Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis

2007

Torres-Bacete, J.; Hormigo, D.; Stuart, M.; Arroyo, M.; Torres, P.; Castillon, M.P.; Acebal, C.; Garcia, J.L.; de la Mata, I.

Appl. Environ. Microbiol.

73

5378-5381

711057

-

Kinetic and microstructural characterization of immobilized penicillin acylase from Streptomyces lavendulae on Sepabeads EC-EP

2009

Hormigo, D.; De La Mata, I.; Castillon, M.; Acebal, C.; Arroyo, M.

Biocatal. Biotransform.

27

271-281

711493

20188542

Immobilized aculeacin A acylase from Actinoplanes utahensis: characterization of a novel biocatalyst

2010

Hormigo, D.; de la Mata, I.; Acebal, C.; Arroyo, M.

Biores. Technol.

101

4261-4268

733165

23263966

Engineering the substrate specificity of a thermophilic penicillin acylase from Thermus thermophilus

2013

Torres, L.L.; Cantero, A.; del Valle, M.; Marina, A.; Lopez-Gallego, F.; Guisan, J.M.; Berenguer, J.; Hidalgo, A.

Appl. Environ. Microbiol.

79

1555-1562

752541

25501472

Overexpression of penicillin V acylase from Streptomyces lavendulae and elucidation of its catalytic residues

2015

Torres-Bacete, J.; Hormigo, D.; Torres-Guzman, R.; Arroyo, M.; Castillon, M.; Garcia, J.; Acebal, C.; de la Mata, I.

Appl. Environ. Microbiol.

81

1225-1233

Links to other databases for Penicillin K

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Show Links (1 entry)

ChEBI

PubChem