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Information on EC 7.6.2.3 - ABC-type glutathione-S-conjugate transporter and Organism(s) Saccharomyces cerevisiae and UniProt Accession P39109

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IUBMB Comments
A eukaryotic ATP-binding cassette (ABC) type transporter that mediates the transport of glutathione-S-conjugates. The mammalian enzyme, which also transports some glucuronides, exports the substrates out of the cell, while plant and fungal transporters export them into the vacuole. Over-expression confers resistance to anticancer drugs by their efficient exportation in glutathione-S-conjugate form.
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Saccharomyces cerevisiae
UNIPROT: P39109
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Synonyms
organic anion transporting polypeptide, rlip76, mrp-1, oatp2, oatp1, abcc5, gsh transporter, hgt1p, multidrug resistance associated protein 2, mrp transporter, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cadmium factor protein
-
glutathione S-conjugate pump
-
vacuolar glutathione S-conjugate transporter
-
ABCC1
-
-
-
-
bile pigment transporter-1
-
-
glutathione-S-conjugate-translocating ATPase
-
-
-
-
high affinity glutathione transporter
-
-
MRP
-
-
-
-
MRP1
-
-
-
-
multidrug resistance-associated protein 1
-
-
-
-
YBT1
-
-
-
-
Ycf1
-
-
-
-
yeast cadmium factor-1
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP + H2O + glutathione-S-conjugate[side 1] = ADP + phosphate + glutathione-S-conjugate[side 2]
A eukaryotic ATP-binding cassette (ABC) type transporter that mediates the transport of glutathione-S-conjugates. The mammalian enzyme, which also transports some glucuronides, exports the substrates out of the cell, while plant and fungal transporters export them into the vacuole. Over-expression confers resistance to anticancer drugs by their efficient exportation in glutathione-S-conjugate form.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + glutathione-S-conjugate[side 1]
ADP + phosphate + glutathione-S-conjugate[side 2]
show the reaction diagram
-
-
-
?
ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side 1]
ADP + phosphate + S-(2,4-dinitrophenyl)glutathione[side 2]
show the reaction diagram
-
-
-
?
ATP + H2O + S-(azidophenacyl)glutathione[side 1]
ADP + phosphate + S-(azidophenacyl)glutathione[side 2]
show the reaction diagram
-
-
-
?
ATP + H2O + S-(metolachlor)glutathione[side 1]
ADP + phosphate + S-(metolachlor)glutathione[side 2]
show the reaction diagram
-
-
-
?
ATP + H2O + S-(monochlorobimane)glutathione[side 1]
ADP + phosphate + S-(monochlorobimane)glutathione[side 2]
show the reaction diagram
-
-
-
?
ATP + H2O + glutathione-S-3-mercaptohexan-1-ol[side 1]
ADP + phosphate + glutathione-S-3-mercaptohexan-1-ol[side 2]
show the reaction diagram
-
-
-
-
?
ATP + H2O + glutathione-S-4-mercapto-4-methylpentan-2-one[side 1]
ADP + phosphate + glutathione-S-4-mercapto-4-methylpentan-2-one[side 2]
show the reaction diagram
-
-
-
-
?
ATP + H2O + glutathione-S-conjugate[side 1]
ADP + phosphate + glutathione-S-conjugate[side 2]
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + glutathione-S-conjugate[side 1]
ADP + phosphate + glutathione-S-conjugate[side 2]
show the reaction diagram
-
-
-
?
ATP + H2O + glutathione-S-conjugate[side 1]
ADP + phosphate + glutathione-S-conjugate[side 2]
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required in form of MgATP
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione disulfide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0865
ATP
at pH 8.0 and 25°C
0.0141
S-(2,4-dinitrophenyl)glutathione[side 1]
at pH 8.0 and 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1791
vanadate
Saccharomyces cerevisiae
at pH 8.0 and 25°C
0.02026
verapamil
Saccharomyces cerevisiae
at pH 8.0 and 25°C
0.0888
vinblastine
Saccharomyces cerevisiae
at pH 8.0 and 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is required for increased resistance to the toxic effects of the exogenous glutathione S-conjugate precursor, 1-chloro-2,4-dinitrobenzene, as well as cadmium
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cordente, A.; Capone, D.; Curtin, C.
Unravelling glutathione conjugate catabolism in Saccharomyces cerevisiae the role of glutathione/dipeptide transporters and vacuolar function in the release of volatile sulfur compounds 3-mercaptohexan-1-ol and 4-mercapto-4-methylpentan-2-one
Appl. Microbiol. Biotechnol.
99
9709-9722
2015
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Manually annotated by BRENDA team
Li, Z.; Szczypka, M.; Lu, Y.; Thiele, D.; Rea, P.
The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump
J. Biol. Chem.
271
6509-6517
1996
Saccharomyces cerevisiae (P39109), Saccharomyces cerevisiae, Saccharomyces cerevisiae DTY165 (P39109)
Manually annotated by BRENDA team
Ballatori, N.; Hammond, C.; Cunningham, J.; Krance, S.; Marchan, R.
Molecular mechanisms of reduced glutathione transport Role of the MRP/CFTR/ABCC and OATP/SLC21A families of membrane proteins
Toxicol. Appl. Pharmacol.
204
238-255
2005
Saccharomyces cerevisiae, Rattus norvegicus, Homo sapiens (O15439), Homo sapiens (O15440), Homo sapiens (P33527), Homo sapiens (Q92887), Homo sapiens
Manually annotated by BRENDA team