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Information on EC 7.6.2.2 - ABC-type xenobiotic transporter and Organism(s) Lactococcus lactis and UniProt Accession P97046

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IUBMB Comments
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. Does not undergo phosphorylation during the transport process. The enzymes from Gram-positive bacteria and eukaryotic cells export a number of drugs with unusual specificity, covering various groups of unrelated substances while ignoring some that are closely related structurally. Several distinct enzymes may be present in a single eukaryotic cell. Many of them also transport glutathione---drug conjugates (see EC 7.6.2.3, ABC-type glutathione-S-conjugate transporter) while others also show some 'flippase' activity (cf. EC 7.6.2.1, P-type phospholipid transporter).
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This record set is specific for:
Lactococcus lactis
UNIPROT: P97046
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The taxonomic range for the selected organisms is: Lactococcus lactis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
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ATP
+
H2O
+
xenobiotic[side 1]
=
ADP
+
phosphate
+
xenobiotic[side 2]
+
+
xenobiotic[side 1]
=
+
+
xenobiotic[side 2]
Synonyms
p-glycoprotein, abcb1, abcg2, atp-binding cassette transporter, abcc2, breast cancer resistance protein, abcc1, multidrug resistance-associated protein, mdr1a, multidrug transporter, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
YdaG/YdbA
heterodimeric ABC multidrug transporter
ATP phosphohydrolase (xenobiotic-exporting)
-
-
-
-
MRP
-
-
-
-
multidrug-resistance protein
-
-
-
-
P-glycoprotein
-
-
-
-
Pgp
-
-
-
-
transporter protein MRP
-
-
-
-
xenobiotic-transporting ATPase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (ABC-type, xenobiotic-exporting)
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. Does not undergo phosphorylation during the transport process. The enzymes from Gram-positive bacteria and eukaryotic cells export a number of drugs with unusual specificity, covering various groups of unrelated substances while ignoring some that are closely related structurally. Several distinct enzymes may be present in a single eukaryotic cell. Many of them also transport glutathione---drug conjugates (see EC 7.6.2.3, ABC-type glutathione-S-conjugate transporter) while others also show some 'flippase' activity (cf. EC 7.6.2.1, P-type phospholipid transporter).
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + 2',7'-bis-(2-carboxyethyl)-5,6-carboxylfluoresceinacetoxymethyl ester/in
ADP + phosphate + 2',7'-bis-(2-carboxyethyl)-5,6-carboxylfluoresceinacetoxymethyl ester/out
show the reaction diagram
YdaG/YdbA heterodimer
-
-
?
ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in
ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out
show the reaction diagram
Hoechst 33342, YdaG/YdbA heterodimer
-
-
?
ATP + H2O + daunomycin/in
ADP + phosphate + daunomycin/out
show the reaction diagram
YdaG/YdbA heterodimer
-
-
?
ATP + H2O + ethidium bromide/in
ADP + phosphate + ethidium bromide/out
show the reaction diagram
YdaG/YdbA heterodimer
-
-
?
ATP + H2O + xenobiotic/in
ADP + phosphate + xenobiotic/out
show the reaction diagram
YdaG and YdbA are two ABC multidrug half-transporters, ATPase activity is associated with the YdaG/YdbA heterodimer only and not with the individual subunits
-
-
?
ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in
ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out
show the reaction diagram
-
Hoechst 33342
-
-
?
ATP + H2O + daunomycin/in
ADP + phosphate + daunomycin/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + ethidium bromide/in
ADP + phosphate + ethidium bromide/out
show the reaction diagram
-
recombinant LmrA, expressed in Escherichia coli WD2
-
-
?
ATP + H2O + Lipid A/in
ADP + phosphate + Lipid A/out
show the reaction diagram
-
native LmrA
-
-
?
ATP + H2O + vinblastine/in
ADP + phosphate + vinblastine/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + xenobiotic/in
ADP + phosphate + xenobiotic/out
show the reaction diagram
-
ABC multidrug transporter LmrA, substrate specificity
-
-
?
daunomycin/in + ATP + H2O
daunomycin/out + ADP + phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethidium
-
-
ortho-vanadate
-
-
reserpine
-
-
rhodamine 6G
-
-
tetraphenylphosphonium
-
-
vanadate
-
vanadate-sensitive ATPase activity
verapamil
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
lipid A
-
stimulates ATPase activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at, ATPase activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at, ATPase activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain IL1403
Uniprot
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
63000
1 * 63000 + 1 * 74000, YdaG +YdbA, a stable heterodimer, SDS-PAGE
74000
1 * 63000 + 1 * 74000, YdaG +YdbA, a stable heterodimer, SDS-PAGE
64613
-
x * 64613, calculation from nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
1 * 63000 + 1 * 74000, YdaG +YdbA, a stable heterodimer, SDS-PAGE
?
-
x * 64613, calculation from nucleotide sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-purification of YdaG and YdbA
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ydaG and ydbA genes, separate expression in Lactococcus lactis NZ9000 using the nisin-inducible system
expression in Escherichia coli
-
lmrA gene, expression in Escherichia coli WD2
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Van Veen, H.W.; Konings, W.N.
The ABC family of multidrug transporters in microorganisms
Biochim. Biophys. Acta
1365
31-36
1998
Bacillus subtilis, Saccharomyces cerevisiae, Caenorhabditis elegans, Candida albicans, Cricetulus griseus, Escherichia coli, Entamoeba histolytica, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Levilactobacillus brevis, Lactococcus lactis, Leishmania donovani, Leishmania tarentolae, Staphylococcus aureus, Mycoplasma genitalium, Plasmodium falciparum
Manually annotated by BRENDA team
Van Veen, H.W.; Venema, K.; Bolhuis, H.; Oussenko, I.; Kok, J.; Poolman, B.; Driessen, A.J.M.; Konings, W.N.
Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1
Proc. Natl. Acad. Sci. USA
93
10668-10672
1996
Lactococcus lactis
Manually annotated by BRENDA team
Reuter, G.; Janvilisri, T.; Venter, H.; Shahi, S.; Balakrishnan, L.; van Veen, H.W.
The ATP binding cassette multidrug transporter LmrA and lipid transporter MsbA have overlapping substrate specificities
J. Biol. Chem.
278
35193-35198
2003
Lactococcus lactis, Lactococcus lactis NZ9000
Manually annotated by BRENDA team
Lubelski, J.; Mazurkiewicz, P.; van Merkerk, R.; Konings, W.N.; Driessen, A.J.M.
ydaG and ydbA of Lactococcus lactis encode a heterodimeric ATP-binding cassette-type multidrug transporter
J. Biol. Chem.
279
34449-34455
2004
Lactococcus lactis (P97046), Lactococcus lactis, Lactococcus lactis MG1363 (P97046)
Manually annotated by BRENDA team