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ATP + H2O + phosphatidylcholine/in
ADP + phosphate + phosphatidylcholine/out
-
-
-
?
ATP + H2O + phosphatidylethanolamine/in
ADP + phosphate + phosphatidylethanolamine/out
-
-
-
?
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
ATP + H2O + 1-palmitoyl-2-(6-(7-nitro-2-1,3-benzoxadiazole)-aminocaproyl)-phosphatidylcholine/out
ADP + phosphate + 1-palmitoyl-2-(6-(7-nitro-2-1,3-benzoxadiazole)-aminocaproyl)-phosphatidylcholine/in
-
internalization through the plasma membrane by the Dnf1p-Lem3p complex
-
-
?
ATP + H2O + 1-palmitoyl-2-(6-(7-nitro-2-1,3-benzoxadiazole)-aminocaproyl)-phosphatidylethanolamine/out
ADP + phosphate + 1-palmitoyl-2-(6-(7-nitro-2-1,3-benzoxadiazole)-aminocaproyl)-phosphatidylethanolamine/in
-
internalization through the plasma membrane by the Dnf1p-Lem3p complex
-
-
?
ATP + H2O + aminophospholipid[side 1]
ADP + phosphate + aminophospholipid[side 2]
-
-
-
-
?
ATP + H2O + glycerophospholipid[side 1]
ADP + phosphate + glycerophospholipid[side 2]
-
-
-
-
?
ATP + H2O + lysophosphatidylcholine/out
ADP + phosphate + lysophosphatidylcholine/in
-
lysophosphatidylcholine is a bona fide biological substrate transported by the yeast plasma membrane ATPases, Dnf1p and Dnf2p, in consort with a second protein Lem3p
-
-
?
ATP + H2O + lysophosphatidylethanolamine/out
ADP + phosphate + lysophosphatidylethanolamine/in
-
lysophosphatidylethanolamine is a bona fide biological substrate transported by the yeast plasma membrane ATPases, Dnf1p and Dnf2p, in consort with a second protein Lem3p
-
-
?
ATP + H2O + phosphatidylcholine/in
ADP + phosphate + phosphatidylcholine/out
ATP + H2O + phosphatidylcholine[side 1]
ADP + phosphate + phosphatidylcholine[side 2]
-
-
-
-
?
ATP + H2O + phosphatidylethanolamine/in
ADP + phosphate + phosphatidylethanolamine/out
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
ATP + H2O + phosphatidylethanolamine[side 1]
ADP + phosphate + phosphatidylethanolamine[side 2]
-
-
-
-
?
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
ATP + H2O + phosphatidylserine[side 1]
ADP + phosphate + phosphatidylserine[side 2]
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
ATP + H2O + phospholipid[side 1]
ADP + phosphate + phospholipid[side 2]
-
-
-
-
?
ATP + H2O + sphingomyelin[side 1]
ADP + phosphate + sphingomyelin[side 2]
-
low activity
-
-
?
Man5GlcNAc2-diphosphate-dolichol/out
Man5GlcNAc2-diphosphate-dolichol/in
-
-
-
-
?
phospholipid/in
phospholipid/out
additional information
?
-
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
-
-
-
?
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
preferred substrate of Drs2
-
-
?
ATP + H2O + phosphatidylcholine/in
ADP + phosphate + phosphatidylcholine/out
-
palmitoyl-(NBD-hexanoyl)-PC , selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry
-
-
?
ATP + H2O + phosphatidylcholine/in
ADP + phosphate + phosphatidylcholine/out
preferred substrate of Dnf1
-
-
?
ATP + H2O + phosphatidylethanolamine/in
ADP + phosphate + phosphatidylethanolamine/out
-
-
-
?
ATP + H2O + phosphatidylethanolamine/in
ADP + phosphate + phosphatidylethanolamine/out
-
selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry
-
-
?
ATP + H2O + phosphatidylethanolamine/in
ADP + phosphate + phosphatidylethanolamine/out
-
palmitoyl-(NBD-hexanoyl)-PE , selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry
-
-
?
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
-
?
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
-
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
-
-
?
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
-
transport from the luminal leaflet of the membrane to the cytosolic leaflet
-
-
?
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
-
7-nitro-2-1,3-benzoxadiazol-4-yl-fluorescent-labeled substrate, transport from the luminal leaflet of the membrane to the cytosolic leaflet
-
-
?
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
-
-
-
?
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
-
-
-
?
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
-
selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry
-
-
?
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
-
palmitoyl-(NBD-hexanoyl)-PS , selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry
-
-
?
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
-
-
-
-
?
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
-
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
-
-
?
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
-
transport from the luminal leaflet of the membrane to the cytosolic leaflet
-
-
?
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
-
7-nitro-2-1,3-benzoxadiazol-4-yl-fluorescent-labeled substrate, transport from the luminal leaflet of the membrane to the cytosolic leaflet
-
-
?
ATP + H2O + phosphatidylserine[side 1]
ADP + phosphate + phosphatidylserine[side 2]
-
-
-
-
?
ATP + H2O + phosphatidylserine[side 1]
ADP + phosphate + phosphatidylserine[side 2]
-
very low activity
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
-
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
-
-
-
ir
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
Dnf1p and Dnf2p are necessary for the internalization phospholipids across the plasma membrane of Saccharomyces cerevisiae, overview. ATP hydrolysis is not sufficient for phospholipid flip in the absence of the proton electrochemical gradient across the plasma membrane, the proton electrochemical gradient is required for phospholipid flip across the plasma membrane of yeast
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
P4-ATPases Drs2p and Dnf1p cycle between the exocytic and endocytic pathways, and maintain a steady-state localization to internal organelles requiring endocytosis signals, Drs2p is an essential endocytic cargo in cells compromised for Ub-dependent endocytosis
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
phospholipid translocases are involved in the generation of phospholipid asymmetry in membrane bilayers
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
the enzyme is involved in protein trafficking, Drs2p acts in complex with the allelic CDC50 protein in ATP-dependent phosphatidylserine translocation playing a role in restricting the substrate to the cytosolic leaflet of the Golgi and the plasma membrane, overview
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases
-
-
ir
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
ATP-dependent transport of phospholipids from outer to the inner monolayer of the cell membrane to maintain lipid asymmetry, the C-terminal and N-terminal NPFXD motifs ar functionally required by Drs2p and Drf1p, respectively, overview
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
ATP-dependent transport of phospholipids from outer to the inner monolayer of the cell membrane to maintain lipid asymmetry, usage of fluorescent labeled 7-nitrobenz-2-oxa-1,3-diazol-4-yl-labeled, NBD-labeled, phospholipid substrate. ATP hydrolysis is not sufficient for phospholipid flip in the absence of the proton electrochemical gradient across the plasma membrane, the proton electrochemical gradient is required for phospholipid flip across the plasma membrane of yeast
-
-
?
phospholipid/in
phospholipid/out
-
-
-
-
ir
phospholipid/in
phospholipid/out
-
transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases
-
-
ir
additional information
?
-
-
The enzyme is essential for inward translocation of NBD-labeled phosphatidylethanolamine, phosphatidylserine and phosphatidylcholine across the plasmamembrane.
-
-
?
additional information
?
-
-
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane, especially Neo1p is important, Drs2p is involved in vesicle formation in the Golgi and thus influences the plasma membrane distribution of aminophospholipids despite its localization the Golgi membrane, mechanism
-
-
?
additional information
?
-
-
the Lem3p-Dnf1p complex can substitute for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments
-
-
?
additional information
?
-
-
Dnf1p forms a complex with Lem3p acting as aminophospholipid translocase
-
-
?
additional information
?
-
-
the enzyme depends on ATP
-
-
?
additional information
?
-
-
endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway, overview. The homologous proteins Cdc50p, Lem3p, and Crf1p are potential noncatalytic subunits of Drs2p, Dnf1p and Dnf2p, and Dnf3p, respectively, these putative heteromeric PLTs share an essential function for cell growth, overview
-
-
?
additional information
?
-
-
functional co-dependence between Drs2p and the AP-1 clathrin adaptor in protein sorting at the trans-Golgi network and early endosomes of Saccharomyces cerevisiae, Drs2p and AP-1 operate in the same pathway and that AP-1 requires Drs2p for function, Drs2p physically interacts with AP-1, overview
-
-
?
additional information
?
-
-
loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, overview
-
-
?
additional information
?
-
-
Cdc50 family proteins form complexes with Drs2 family proteins, overview
-
-
?
additional information
?
-
-
complex formation of Drs2p with CDC50, overview
-
-
?
additional information
?
-
-
no transport of (NBD-hexanoyl)-sphingosine-1-phosphocholine
-
-
?
additional information
?
-
-
shape change of giant unilamellar vesicles can be used as a tool to study the occurrence and time scale of flippase-mediated transbilayer movement of unlabeled phospholipids, overview
-
-
?
additional information
?
-
-
isozyme Drs2p interacts with the guanine nucleotide exchange factor Gea2p involved in activation of ADP-ribosylation factors
-
-
?
additional information
?
-
-
isozyme Neo1p interacts with the guanine nucleotide exchange factor Ysl2p/Mon2p and the Arf Arl1p, and cooperation between these three proteins is important for recruitment of clathrin adaptors
-
-
?
additional information
?
-
wild type Dnf1 cannot translocate NBD-SM or NBD-PA
-
-
?
additional information
?
-
wild type Dnf1 cannot translocate NBD-SM or NBD-PA
-
-
?
additional information
?
-
-
wild type Dnf1 cannot translocate NBD-SM or NBD-PA
-
-
?
additional information
?
-
-
no activity with phosphatidic acid
-
-
?
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ATP + H2O + aminophospholipid[side 1]
ADP + phosphate + aminophospholipid[side 2]
-
-
-
-
?
ATP + H2O + glycerophospholipid[side 1]
ADP + phosphate + glycerophospholipid[side 2]
-
-
-
-
?
ATP + H2O + phosphatidylethanolamine/in
ADP + phosphate + phosphatidylethanolamine/out
-
selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry
-
-
?
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
ATP + H2O + phosphatidylserine/in
ADP + phosphate + phosphatidylserine/out
-
selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry
-
-
?
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
ATP + H2O + phospholipid[side 1]
ADP + phosphate + phospholipid[side 2]
-
-
-
-
?
phospholipid/in
phospholipid/out
-
transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases
-
-
ir
additional information
?
-
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
-
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
-
-
?
ATP + H2O + phosphatidylethanolamine/out
ADP + phosphate + phosphatidylethanolamine/in
-
transport from the luminal leaflet of the membrane to the cytosolic leaflet
-
-
?
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
-
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
-
-
?
ATP + H2O + phosphatidylserine/out
ADP + phosphate + phosphatidylserine/in
-
transport from the luminal leaflet of the membrane to the cytosolic leaflet
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
-
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
Dnf1p and Dnf2p are necessary for the internalization phospholipids across the plasma membrane of Saccharomyces cerevisiae, overview. ATP hydrolysis is not sufficient for phospholipid flip in the absence of the proton electrochemical gradient across the plasma membrane, the proton electrochemical gradient is required for phospholipid flip across the plasma membrane of yeast
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
P4-ATPases Drs2p and Dnf1p cycle between the exocytic and endocytic pathways, and maintain a steady-state localization to internal organelles requiring endocytosis signals, Drs2p is an essential endocytic cargo in cells compromised for Ub-dependent endocytosis
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
phospholipid translocases are involved in the generation of phospholipid asymmetry in membrane bilayers
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
the enzyme is involved in protein trafficking, Drs2p acts in complex with the allelic CDC50 protein in ATP-dependent phosphatidylserine translocation playing a role in restricting the substrate to the cytosolic leaflet of the Golgi and the plasma membrane, overview
-
-
?
ATP + H2O + phospholipid/in
ADP + phosphate + phospholipid/out
-
transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases
-
-
ir
additional information
?
-
-
The enzyme is essential for inward translocation of NBD-labeled phosphatidylethanolamine, phosphatidylserine and phosphatidylcholine across the plasmamembrane.
-
-
?
additional information
?
-
-
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane, especially Neo1p is important, Drs2p is involved in vesicle formation in the Golgi and thus influences the plasma membrane distribution of aminophospholipids despite its localization the Golgi membrane, mechanism
-
-
?
additional information
?
-
-
the Lem3p-Dnf1p complex can substitute for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments
-
-
?
additional information
?
-
-
endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway, overview. The homologous proteins Cdc50p, Lem3p, and Crf1p are potential noncatalytic subunits of Drs2p, Dnf1p and Dnf2p, and Dnf3p, respectively, these putative heteromeric PLTs share an essential function for cell growth, overview
-
-
?
additional information
?
-
-
functional co-dependence between Drs2p and the AP-1 clathrin adaptor in protein sorting at the trans-Golgi network and early endosomes of Saccharomyces cerevisiae, Drs2p and AP-1 operate in the same pathway and that AP-1 requires Drs2p for function, Drs2p physically interacts with AP-1, overview
-
-
?
additional information
?
-
-
loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, overview
-
-
?
additional information
?
-
-
isozyme Drs2p interacts with the guanine nucleotide exchange factor Gea2p involved in activation of ADP-ribosylation factors
-
-
?
additional information
?
-
-
isozyme Neo1p interacts with the guanine nucleotide exchange factor Ysl2p/Mon2p and the Arf Arl1p, and cooperation between these three proteins is important for recruitment of clathrin adaptors
-
-
?
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F511L
the mutant has a specific activity that is 35% that of wild type Drs2
F511Y
the mutant retains wild type activity, the substitution in Drs2 specifically abrogates phosphatidylserine recognition by this flippase causing phosphatidylserine exposure on the outer leaflet of the plasma membrane without disrupting phospatidylethanolamine asymmetry
D256E
-
the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme
F213S
-
the mutant shows increased activity with phosphatidylcholine, phosphatidylserine, and sphingomyelin compared to the wild type enzyme
I1235F
mutation increases the overall activity of Dnf1 for all substrates and causes partial loss of specificity for glycerophospholipid
I615M
-
the mutant shows about wild type activity
I615S
-
the mutation significantly increases sphingomyelin and phosphatidylserine transport compared to the wild type enzyme
I615T
-
the mutant shows about wild type activity
L242S
-
the mutant shows reduced activity with phosphatidylcholine and wild type activity with sphingomyelin
N220C
-
the mutant shows wild type activity with phosphatidylcholine and increased activity with sphingomyelin
N220S
-
the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme
N220S/L242S
-
the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme
N220T
-
the mutant shows increased activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme
N550S
-
the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme
T254A
-
the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme
Y618F
acquisition of the phosphatidylserine substrate maps to a Tyr618Phe substitution in transmembrane segment 4 of Dnf1. The rate of 7-nitrobenz-2oxa-1,3-diazol-4-yl phospholipid uptake by Dnf1 Y618F is comparable to wild type Dnf1
additional information
generation of chimeras between isoforms Drs2 and Dnf1 by swapping of transmembrane domains. The mechanistic coupling of the entry and the exit gates seems to be conserved for Drs2. The residues that determine headgroup specificity for phospholipid transport cluster at two interfacial regions flanking transmembrane segments 1-4 and lie outside of the canonical substrate binding site operating in cation pumps. Two substrate-selecting gates act sequentially on opposite sides of the membrane: an entry gate, where phospholipid is initially selected from the extracellular leaflet, and an exit gate at the cytosolic leaflet. The entry and exit gates act cooperatively but imperfectly For example, the entry gate QQ to GA mutation causes a specific loss of phosphatidylserine asymmetry that can be restored by the N445S exit gate mutation
additional information
generation of chimeras between isoforms Drs2 and Dnf1 by swapping of transmembrane domains. The mechanistic coupling of the entry and the exit gates seems to be conserved for Drs2. The residues that determine headgroup specificity for phospholipid transport cluster at two interfacial regions flanking transmembrane segments 1-4 and lie outside of the canonical substrate binding site operating in cation pumps. Two substrate-selecting gates act sequentially on opposite sides of the membrane: an entry gate, where phospholipid is initially selected from the extracellular leaflet, and an exit gate at the cytosolic leaflet. The entry and exit gates act cooperatively but imperfectly For example, the entry gate QQ to GA mutation causes a specific loss of phosphatidylserine asymmetry that can be restored by the N445S exit gate mutation
additional information
-
lem3 mutants are not affected in Lem3p-Dnf1p complex formation, but show a synthetic growth defect with the null mutation CDC50, overview
additional information
-
simultaneous inactivation of Dnf2p and Dnf1p abolishes the ATP-dependent influx of phosphatidylserine and phosphatidylethanolamine into the cell
additional information
-
a conditional allele that inactivates Drs2p phospholipid translocase activity disrupts its own transport in this AP-1 pathway, missorting of Drs2p to the plasma membrane of AP-1 mutants, cell surface accumulation of GFP-Drs2p is less pronounced in apm1DELTA and nearly absent in apm2DELTA cells treated with latrunculin A, overview
additional information
-
construction of deletion mutants of phosphilipid translocases and CDC50 proteins, phenotypes, detailed overview
additional information
-
construction of yeast knockout strains lacking Drs2p and/or Drf1p activity, the pan1-20 temperature-sensitive mutant is constitutively defective for Ub-dependent endocytosis but is not defective for NPFXD-dependent endocytosis at the permissive growth temperature. To sustain viability of pan1-20, Drs2p must be endocytosed through the NPFXD/Sla1p pathway
additional information
-
deletion mutants of drs2 and cdc50, DELTAdrs2 and DELTAcdc50, exhibit a clathrin-deficient phenotype with delayed transport of carboxypeptidase Y to the vacuole, mislocation of resident TGN enzymes and the accumulation of aberrant membrane structures, the mutations cause a loss in plasma membrane phosphatidylserine asymmetry leading to disruption of protein trafficking, phenotypes, overview
additional information
-
loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, removal of P4 ATPases Dnf1p and Dnf2p from budding yeast abolishes inward translocation of 6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl] (NBD)-labeled phosphatidylserine, phosphatidylethanolamine, and phosphatidylcholine across the plasma membrane and causes cell surface exposure of endogenous phosphatidylethanolamine, overview
additional information
generation of chimeras between isoforms Drs2 and Dnf1 by swapping of transmembrane domains. Within the entry gate, residues specifically involved in the phosphatidylcholine preference of Dnf1 are Ile234, Phe235, Pro240, Gly241, Tyr575, Glu586, Phe587, Gly588, and Ile590, and involved in suppression of phosphatidylserine recognition are Gly230, Ala231, and K578. Within the exit gate, residues responsible for phosphatidylcholine preference are Ile545 and Glu622 and for suppression of phosphatidylserine transport are Tyr618, Asn550, Val621, and Glu622
additional information
generation of chimeras between isoforms Drs2 and Dnf1 by swapping of transmembrane domains. Within the entry gate, residues specifically involved in the phosphatidylcholine preference of Dnf1 are Ile234, Phe235, Pro240, Gly241, Tyr575, Glu586, Phe587, Gly588, and Ile590, and involved in suppression of phosphatidylserine recognition are Gly230, Ala231, and K578. Within the exit gate, residues responsible for phosphatidylcholine preference are Ile545 and Glu622 and for suppression of phosphatidylserine transport are Tyr618, Asn550, Val621, and Glu622
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Pomorski, T.; Lombardi, R.; Riezman, H.; Devaux, P.F.; van Meer, G.; Holthuis, J.C.M.
Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis
Mol. Biol. Cell
14
1240-1254
2003
Saccharomyces cerevisiae
brenda
Saito, K.; Fujimura-Kamada, K.; Furuta, N.; Kato, U.; Umeda, M.; Tanaka, K.
Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae
Mol. Biol. Cell
15
3418-3432
2004
Saccharomyces cerevisiae
brenda
Noji, T.; Yamamoto, T.; Saito, K.; Fujimura-Kamada, K.; Kondo, S.; Tanaka, K.
Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast
Biochem. Biophys. Res. Commun.
344
323-331
2006
Saccharomyces cerevisiae
brenda
Devaux, P.F.; Lopez-MOntero, I.; Bryde, S.
Proteins involved in lipid translocation in eukaryotic cells
Chem. Phys. Lipids
141
119-132
2006
Bos taurus, Saccharomyces cerevisiae, Homo sapiens, Leishmania infantum, Rattus norvegicus
brenda
Natarajan, P.; Wang, J.; Hua, Z.; Graham, T.R.
Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function
Proc. Natl. Acad. Sci. USA
101
10614-10619
2004
Saccharomyces cerevisiae
brenda
Papadopulos, A.; Vehring, S.; Lopez-Montero, I.; Kutschenko, L.; Stoeckl, M.; Devaux, P.F.; Kozlov, M.; Pomorski, T.; Herrmann, A.
Flippase activity detected with unlabeled lipids by shape changes of giant unilamellar vesicles
J. Biol. Chem.
282
15559-15568
2007
Saccharomyces cerevisiae, Saccharomyces cerevisiae sec61
brenda
Stevens, H.C.; Nichols, J.W.
The proton electrochemical gradient across the plasma membrane of yeast is necessary for phospholipid flip
J. Biol. Chem.
282
17563-17567
2007
Saccharomyces cerevisiae, Saccharomyces cerevisiae LMY65
brenda
Alder-Baerens, N.; Lisman, Q.; Luong, L.; Pomorski, T.; Holthuis, J.C.
Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles
Mol. Biol. Cell
17
1632-1642
2006
Saccharomyces cerevisiae, Saccharomyces cerevisiae EHY227
brenda
Furuta, N.; Fujimura-Kamada, K.; Saito, K.; Yamamoto, T.; Tanaka, K.
Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway
Mol. Biol. Cell
18
295-312
2007
Saccharomyces cerevisiae
brenda
Liu, K.; Hua, Z.; Nepute, J.A.; Graham, T.R.
Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway
Mol. Biol. Cell
18
487-500
2007
Saccharomyces cerevisiae
brenda
Liu, K.; Surendhran, K.; Nothwehr, S.F.; Graham, T.R.
P4-ATPase requirement for AP-1/clathrin function in protein transport from the trans-Golgi network and early endosomes
Mol. Biol. Cell
19
3525-3535
2008
Saccharomyces cerevisiae
brenda
Chen, S.; Wang, J.; Muthusamy, B.; Liu, K.; Zare, S.; Andersen, R.J.; Graham, T.R.
Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane
Traffic
7
1503-1517
2006
Saccharomyces cerevisiae, Saccharomyces cerevisiae CCY 2811
brenda
Sanyal, S.; Frank, C.G.; Menon, A.K.
Distinct flippases translocate glycerophospholipids and oligosaccharide diphosphate dolichols across the endoplasmic reticulum
Biochemistry
47
7937-7946
2008
Saccharomyces cerevisiae, Saccharomyces cerevisiae YCF40
brenda
Riekhof, W.R.; Voelker, D.R.
The yeast plasma membrane P4-ATPases are major transporters for lysophospholipids
Biochim. Biophys. Acta
179
620-627
2009
Saccharomyces cerevisiae
brenda
Poulsen, L.R.; Lopez-Marques, R.L.; Palmgren, M.G.
Flippases: still more questions than answers
Cell. Mol. Life Sci.
65
3119-3125
2008
Saccharomyces cerevisiae
brenda
Muthusamy, B.P.; Raychaudhuri, S.; Natarajan, P.; Abe, F.; Liu, K.; Prinz, W.A.; Graham, T.R.
Control of protein and sterol trafficking by antagonistic activities of a P4-ATPase and oxysterol binding protein homologue
Mol. Biol. Cell
20
2920-2931
2009
Saccharomyces cerevisiae
brenda
Takagi, K.; Iwamoto, K.; Kobayashi, S.; Horiuchi, H.; Fukuda, R.; Ohta, A.
Involvement of Golgi-associated retrograde protein complex in the recycling of the putative Dnf aminophospholipid flippases in yeast
Biochem. Biophys. Res. Commun.
417
490-494
2012
Saccharomyces cerevisiae
brenda
Sebastian, T.T.; Baldridge, R.D.; Xu, P.; Graham, T.R.
Phospholipid flippases: Building asymmetric membranes and transport vesicles
Biochim. Biophys. Acta
1821
1068-1077
2012
Arabidopsis thaliana, Saccharomyces cerevisiae, Saccharomyces cerevisiae (P32660), Saccharomyces cerevisiae (P39524), Saccharomyces cerevisiae (Q12674), Homo sapiens, Caenorhabditis elegans (C7U324), Caenorhabditis elegans (G5EBH1), Caenorhabditis elegans (O18182), Caenorhabditis elegans (P91203), Caenorhabditis elegans (Q7YXV5)
brenda
Baldridge, R.D.; Graham, T.R.
Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases
Proc. Natl. Acad. Sci. USA
109
E290-E298
2012
Saccharomyces cerevisiae (P32660), Saccharomyces cerevisiae (P39524), Saccharomyces cerevisiae
brenda
Baldridge, R.D.; Graham, T.R.
Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases
Proc. Natl. Acad. Sci. USA
110
E358-E367
2013
Saccharomyces cerevisiae (P32660), Saccharomyces cerevisiae (P39524)
brenda
Roland, B.; Graham, T.
Decoding P4-ATPase substrate interactions
Crit. Rev. Biochem. Mol. Biol.
51
513-527
2016
Saccharomyces cerevisiae
brenda
Yamamoto, T.; Fujimura-Kamada, K.; Shioji, E.; Suzuki, R.; Tanaka, K.
Cfs1p, a novel membrane protein in the PQ-loop family, is involved in phospholipid flippase functions in yeast
G3 (Bethesda)
7
179-192
2017
Saccharomyces cerevisiae
brenda
Takatsu, H.; Baba, K.; Shima, T.; Umino, H.; Kato, U.; Umeda, M.; Nakayama, K.; Shin, H.
ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent
J. Biol. Chem.
286
38159-38167
2015
Saccharomyces cerevisiae
brenda
Yamagami, K.; Yamamoto, T.; Sakai, S.; Mioka, T.; Sano, T.; Igarashi, Y.; Tanaka, K.
Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants
PLoS ONE
10
e0120108
2015
Saccharomyces cerevisiae, Saccharomyces cerevisiae YEF473
brenda
Roland, B.P.; Graham, T.R.
Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase
Proc. Natl. Acad. Sci. USA
113
E4460-E4466
2016
Saccharomyces cerevisiae
brenda