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EC Tree
IUBMB Comments An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. The enzyme, found in bacteria, interacts with an extracytoplasmic substrate binding protein and mediates the import of lactose, melibiose and raffinose.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
+
oligosaccharide-[oligosaccharide-binding protein][side 1]
=
+
+
oligosaccharide[side 2]
+
[oligosaccharide-binding protein][side 1]
Synonyms
hlyb nbd,
more
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ABC ATPase domain of HlyB transporter
ABC-type (ATP-binding cassette-type) ATPase
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ATP phosphohydrolase (disaccharide-importing)
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ATP-binding cassette ATPase domain of the haemolysin B transporter
oligosaccharide-transporting ATPase
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ABC ATPase domain of HlyB transporter
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ABC ATPase domain of HlyB transporter
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ATP-binding cassette ATPase domain of the haemolysin B transporter
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ATP-binding cassette ATPase domain of the haemolysin B transporter
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HlyB NBD
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MsmX
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ATP + H2O + oligosaccharide-[oligosaccharide-binding protein][side 1] = ADP + phosphate + oligosaccharide[side 2] + [oligosaccharide-binding protein][side 1]
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hydrolysis of phosphoric ester
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ATP phosphohydrolase (ABC-type, oligosaccharide-importing)
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. The enzyme, found in bacteria, interacts with an extracytoplasmic substrate binding protein and mediates the import of lactose, melibiose and raffinose.
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8-azido-ATP + H2O + ?
8-azido-ADP + phosphate + ?
ATP + H2O + ?
ADP + phosphate + ?
ATP + H2O + arabinan-[arabinan-binding protein][side 1]
ADP + phosphate + arabinan[side 2] + [arabinan-binding protein][side 1]
ATP + H2O + cellobiose/out
ADP + phosphate + cellobiose/in
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two separate uptake systems for cellobiose and maltose, the ATP-binding component MsiK assists in cellobiose and maltose transport
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?
ATP + H2O + D-glucose-[D-glucose-binding protein][side 1]
ADP + phosphate + D-glucose[side 2] + [D-glucose-binding protein][side 1]
ATP + H2O + galactan-[galactan-binding protein][side 1]
ADP + phosphate + galactan[side 2] + [galactan-binding protein][side 1]
ATP + H2O + galacturonic acid-[galacturonic acid-binding protein][side 1]
ADP + phosphate + galacturonic acid[side 2] + [galacturonic acid-binding protein][side 1]
ATP + H2O + isomaltotriose/out
ADP + phosphate + isomaltotriose/in
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
ATP + H2O + maltose/out
ADP + phosphate + maltose/in
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two separate uptake systems for cellobiose and maltose, the ATP-binding component MsiK assists in cellobiose and maltose transport
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?
ATP + H2O + melibiose/out
ADP + phosphate + melibiose/in
ATP + H2O + pectin-[pectin-binding protein][side 1]
ADP + phosphate + pectin[side 2] + [pectin-binding protein][side 1]
ATP + H2O + polygalacturonan-[polygalacturonan-binding protein][side 1]
ADP + phosphate + polygalacturonan[side 2] + [polygalacturonan-binding protein][side 1]
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?
ATP + H2O + raffinose/out
ADP + phosphate + raffinose/in
ATP + H2O + rhamnogalacturonan-[rhamnogalacturonan-binding protein][side 1]
ADP + phosphate + rhamnogalacturonan[side 2] + [rhamnogalacturonan-binding protein][side 1]
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?
8-azido-ATP + H2O + ?
8-azido-ADP + phosphate + ?
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?
8-azido-ATP + H2O + ?
8-azido-ADP + phosphate + ?
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?
ATP + H2O + ?
ADP + phosphate + ?
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?
ATP + H2O + ?
ADP + phosphate + ?
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?
ATP + H2O + arabinan-[arabinan-binding protein][side 1]
ADP + phosphate + arabinan[side 2] + [arabinan-binding protein][side 1]
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?
ATP + H2O + arabinan-[arabinan-binding protein][side 1]
ADP + phosphate + arabinan[side 2] + [arabinan-binding protein][side 1]
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?
ATP + H2O + D-glucose-[D-glucose-binding protein][side 1]
ADP + phosphate + D-glucose[side 2] + [D-glucose-binding protein][side 1]
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low activity
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?
ATP + H2O + D-glucose-[D-glucose-binding protein][side 1]
ADP + phosphate + D-glucose[side 2] + [D-glucose-binding protein][side 1]
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low activity
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?
ATP + H2O + galactan-[galactan-binding protein][side 1]
ADP + phosphate + galactan[side 2] + [galactan-binding protein][side 1]
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?
ATP + H2O + galactan-[galactan-binding protein][side 1]
ADP + phosphate + galactan[side 2] + [galactan-binding protein][side 1]
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-
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?
ATP + H2O + galacturonic acid-[galacturonic acid-binding protein][side 1]
ADP + phosphate + galacturonic acid[side 2] + [galacturonic acid-binding protein][side 1]
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?
ATP + H2O + galacturonic acid-[galacturonic acid-binding protein][side 1]
ADP + phosphate + galacturonic acid[side 2] + [galacturonic acid-binding protein][side 1]
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-
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?
ATP + H2O + isomaltotriose/out
ADP + phosphate + isomaltotriose/in
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?
ATP + H2O + isomaltotriose/out
ADP + phosphate + isomaltotriose/in
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the enzyme system is responsible for the uptake of melibiose, raffinose and isomaltotriose
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?
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
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?
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
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lactose transport
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?
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
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?
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
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lactose transport
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?
ATP + H2O + melibiose/out
ADP + phosphate + melibiose/in
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?
ATP + H2O + melibiose/out
ADP + phosphate + melibiose/in
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the enzyme system is responsible for the uptake of melibiose, raffinose and isomaltotriose
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?
ATP + H2O + pectin-[pectin-binding protein][side 1]
ADP + phosphate + pectin[side 2] + [pectin-binding protein][side 1]
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?
ATP + H2O + pectin-[pectin-binding protein][side 1]
ADP + phosphate + pectin[side 2] + [pectin-binding protein][side 1]
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?
ATP + H2O + raffinose/out
ADP + phosphate + raffinose/in
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?
ATP + H2O + raffinose/out
ADP + phosphate + raffinose/in
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the enzyme system is responsible for the uptake of melibiose, raffinose and isomaltotriose
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?
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ATP + H2O + isomaltotriose/out
ADP + phosphate + isomaltotriose/in
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the enzyme system is responsible for the uptake of melibiose, raffinose and isomaltotriose
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?
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
ATP + H2O + melibiose/out
ADP + phosphate + melibiose/in
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the enzyme system is responsible for the uptake of melibiose, raffinose and isomaltotriose
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?
ATP + H2O + raffinose/out
ADP + phosphate + raffinose/in
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the enzyme system is responsible for the uptake of melibiose, raffinose and isomaltotriose
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?
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
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lactose transport
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?
ATP + H2O + lactose/out
ADP + phosphate + lactose/in
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lactose transport
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?
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Mg2+
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100% activity at pH 8.0 and with 10 mM KCl
Mn2+
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125% activity at pH 8.0 and with 10 mM KCl
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Ca2+
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3% activity at pH 8.0 and 10 mM KCl
Co2+
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47% activity at pH 8.0 and 10 mM KCl
orthovanadate
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0.005-0.05 mM, pH 8.0, IC50: 0.016 mM
Zn2+
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1% activity at pH 8.0 and 10 mM KCl
additional information
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the activity is reversibly inhibited by high salt concentrations in the physiologically range accompanied by proportional decreased binding of 8-azido-ATP (3-5% residual activity at 200-300 mM salt)
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0.65
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 10 mM NaCl
1.09
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 50 mM NaCl
1.5
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 100 mM NaCl
2.33
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 200 mM NaCl
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0.11
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 200 mM NaCl
0.2
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 100 mM NaCl
0.31
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 50 mM NaCl
0.33
ATP
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KM-value with 50% of the enzyme being substrate-saturated, in the presence of 10 mM NaCl
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0.016
orthovanadate
Escherichia coli
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0.005-0.05 mM, pH 8.0, IC50: 0.016 mM
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8
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in the presence of 3 mM MgCl2 and 10 mM KCl
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brenda
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brenda
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brenda
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brenda
strain DH5alpha
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brenda
strain DH5alpha
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brenda
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brenda
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brenda
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brenda
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A0A0B6BRG5_BACTU
280
6
31288
TrEMBL
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A0A243H5I0_BACTU
366
0
41436
TrEMBL
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A0A2X0R954_LACHE
368
0
41286
TrEMBL
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A0A1Y0TKP9_BACTU
433
8
48360
TrEMBL
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G9AGM5_RHIFH
Rhizobium fredii (strain HH103)
377
0
41152
TrEMBL
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27500
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HlyB NBD in the presence of 100 mM KCl and ATP, ultracentrifugation
27700
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HlyB NBD in the presence of 10 mM KCl and in the absence of ATP, ultracentrifugation
28000
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His-tagged HlyB ABC domain, gel filtration
30800
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HlyB NBD in the presence of 10 mM KCl and ATP, ultracentrifugation
35700
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HlyB NBD in the absence of KCl and ATP, ultracentrifugation
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?
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x * 40000, His-tagged enzyme, SDS-PAGE
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x * 40000, His-tagged enzyme, SDS-PAGE
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dimer
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2 * 28000, gel filtration, small amounts of dimers (apparently when stabilized by 8-azido-ATP) are detected even in the presence of salt
dimer
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2 * 28000, gel filtration, small amounts of dimers (apparently when stabilized by 8-azido-ATP) are detected even in the presence of salt
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monomer
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1 * 28000, gel filtration and ultracentrifugation, over a wide range of protein or NaCl or KCl concentrations the enzyme is only detected as monomer
monomer
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1 * 28000, gel filtration and ultracentrifugation, over a wide range of protein or NaCl or KCl concentrations the enzyme is only detected as monomer
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additional information
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lac operon encodes a binding-protein-dependent lactose transport system and beta-galactosidase: 1. lactose binding protein, lacE, MW 42992 calculated from amino acid sequence, 2. integral membrane protein LacF, 3. integral membrane protein lacG, 4. an ATP-binding protein, lacK, MW 39299 calculated from nucleotide sequence, 5. beta galactosidase, lacZ
additional information
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lac operon encodes a binding-protein-dependent lactose transport system and beta-galactosidase: 1. lactose binding protein, lacE, MW 42992 calculated from amino acid sequence, 2. integral membrane protein LacF, 3. integral membrane protein lacG, 4. an ATP-binding protein, lacK, MW 39299 calculated from nucleotide sequence, 5. beta galactosidase, lacZ
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additional information
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the proteins specified by the msm operon includes: 1. alpha-galactosidase, aga, MW 32050 Da determined by calculation from nucleotide sequence, 2. a periplasmic-like sugar-binding protein, msmE, MW 31900 Da determined by calculation from nucleotide sequence, 3.+ 4. two membrane proteins, msmF and msmG, MW for both is 28500 DA as determined by SDS-PAGE, MW 31615 Da for msmG determined by calculation from nucleotide sequence, 5. sucrose phosphorylase, gtfA, 6. an ATP-binding protein, msmK, MW 41960 Da determined by calculation from nucleotide sequence, MW 35000 Da determined by SDS-PAGE and 7. a dextran glucosidase, dexB, MW 48000 Da determined by SDS-PAGE. The operon is controlled by the regulatory gene msmR, MW 26000 Da, determined by SDS-PAGE
additional information
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two separate uptake systems for cellobiose and maltose, the ATP-binding component MsiK assists in cellobiose and maltose transport
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proteolytic modification
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LacE is synthesized as a precursor protein of 422 amino acids, the mature protein is formed by cleavage of the 28 amino acid-N-terminal region
proteolytic modification
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LacE is synthesized as a precursor protein of 422 amino acids, the mature protein is formed by cleavage of the 28 amino acid-N-terminal region
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proteolytic modification
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the msmE gene encodes a proteins of 420 amino acids, the first 22 specifying a signal peptide
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7
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75% activity in the presence of 3 mM MgCl2 and 10 mM KCl
667495
7.5
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90% activity in the presence of 3 mM MgCl2 and 10 mM KCl
667495
8
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100% activity in the presence of 3 mM MgCl2 and 10 mM KCl
667495
8.5
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85% activity in the presence of 3 mM MgCl2 and 10 mM KCl
667495
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-80°C, 50 mM phosphate buffer pH 8, 50 mM KCl and 10% glycerol, 15 mg/ml HlyB ABC domain, indefinite storage duration, no loss of activity
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-80°C, phosphate or Tris buffer pH 8, in the absence of ATP, up to 25 mg/ml His-tagged HlyB ABC domain, indefinite storage duration, no loss of activity
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25°C, 50 mM phosphate buffer pH 8, 50 mM KCl and 10% glycerol, 15 mg/ml HlyB ABC domain, 24h, 20% loss of activity
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4°C, 50 mM phosphate buffer pH 8, 50 mM KCl and 10% glycerol, 15 mg/ml HlyB ABC domain, 2 days, no loss of activity
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4°C, phosphate or Tris buffer pH 8, in the absence of ATP, up to 25 mg/ml His-tagged HlyB ABC domain, at least 2 days, no loss of activity
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HlyB ABC domain, Ni2+-chelating Sepharose 16/20 fast flow column chromatography, His-tagged HlyB ABC domain, immobilized metal-affinity chromatography
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expression in Escherichia coli
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Russell, R.R.B.; Aduse-Opoku, J.; Sutcliffe, I.C.; Tao, L.; Ferretti, J.J.
A binding protein-dependent transport system in Streptococcus mutans responsible for multiple sugar metabolism
J. Biol. Chem.
267
4631-4637
1992
Streptococcus mutans
brenda
Williams, S.G.; Greenwood, J.A.; Jones, C.W.
Molecular analysis of the lac operon encoding the binding-protein-dependent lactose transport system and beta-galactosidase in Agrobacterium radiobacter
Mol. Microbiol.
6
1755-1768
1992
Agrobacterium tumefaciens, Agrobacterium tumefaciens AR50
brenda
Schloesser, A.; Kampers, T.; Schrempf, H.
The Streptomyces ATP-binding component MsiK assists in cellobiose and maltose transport
J. Bacteriol.
179
2092-2095
1997
Streptomyces sp.
brenda
Benabdelhak, H.; Schmitt, L.; Horn, C.; Jumel, K.; Blight, M.A.; Holland, I.B.
Positive co-operative activity and dimerization of the isolated ABC ATPase domain of HlyB from Escherichia coli
Biochem. J.
386
489-495
2005
Escherichia coli, Escherichia coli DH5-alpha
brenda
Ferreira, M.; Mendes, A.; Sa-Nogueira, I.
The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
PLoS ONE
12
e0189483
2017
Bacillus subtilis, Bacillus subtilis 168T+
brenda
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