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Information on EC 7.4.2.5 - bacterial ABC-type protein transporter and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WGP5

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IUBMB Comments
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. This entry stands for a family of bacterial enzymes that are dedicated to the secretion of one or several closely related proteins belonging to the toxin, protease and lipase families. Examples from Gram-negative bacteria include alpha-hemolysin, cyclolysin, colicin V and siderophores, while examples from Gram-positive bacteria include bacteriocin, subtilin, competence factor and pediocin.
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This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WGP5
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The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
pept1, peptide transporter, pept2, seca2, abc transport, seca protein, peptide transporter 1, abcb10, seca1, seca atpase, more
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
transmembrane transport
-
hydrolysis of phosphoric ester
transmembrane transport
hydolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (ABC-type, peptide-exporting)
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. This entry stands for a family of bacterial enzymes that are dedicated to the secretion of one or several closely related proteins belonging to the toxin, protease and lipase families. Examples from Gram-negative bacteria include alpha-hemolysin, cyclolysin, colicin V and siderophores, while examples from Gram-positive bacteria include bacteriocin, subtilin, competence factor and pediocin.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
ATP + H2O + Msmeg1704 in
ADP + phosphate + Msmeg1704 out
show the reaction diagram
-
-
-
ir
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
ATP + H2O + Msmeg1704 in
ADP + phosphate + Msmeg1704 out
show the reaction diagram
-
-
-
ir
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
ATPase activity assay
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
role for the SecA2 pathway in the cell wall localization of solute binding proteins that work with ABC transporters to import solutes. Additionally, SecA2 has a profound effect on the cell wall localization of the Mce1 and Mce4 lipid transporters, which contribute to Mycobacterium tuberculosis virulence. A relationship exists between SecA2 and the hypoxia-induced DosR regulon associated with Mycobacterium tuberculosis latency. Nearly half of the transcriptionally controlled DosR regulon of cytoplasmic proteins are detected at higher levels in the secA2 mutant versus wild-type
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
106000
monomer, predicted molecular mass, determined by SDS-PAGE
85400
monomer, predicted molecular mass, determined by SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
subunit SecA1 forms homodimers with an apparent dimer dissociation constant of 65 nM at 30 mM KCl, microscale thermophoresis technique. No binding is observed at 300 mM KCl. Heterodimerization of subunit SecA1 and SecA2 is observed with an apparent Kd of 378 nM while the experiment in high salt buffer shows no interaction. SecA1/SecA2 heterodimers have a significantly lower affinity than the SecA1 or SecA2 homodimer
dimer
subunit SecA1 forms homodimers with an apparent dimer dissociation constant of Kd of 161 nM at 30 mM KCl, and a Kd of 618 nM at 150 mM KCl, microscale thermophoresis technique. No binding is observed at 300 mM KCl. Heterodimerization of subunit SecA1 and SecA2 is observed with an apparent Kd of 378 nM while the experiment in high salt buffer shows no interaction. SecA1/SecA2 heterodimers have a significantly lower affinity than the SecA1 or SecA2 homodimer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K115A
mutant, substitution of the conserved lysine in the Walker A motif eliminates ATP binding and affects the biological activity
K115R
mutant, substitution of the conserved lysine in the Walker A motif eliminates ATP binding and affects the biological activity
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using a Blue-Sepharose CL-6B column
using a Blue-Sepharose CL-6B column
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
into the pET29a vector for expression in Escherichia coli BL21DE3 cells
expression in Escherichia coli
into the pET41b vector for expression in Escherichia coli BL21DE3 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kurtz, S.; McKinnon, K.P.; Runge, M.S.; Ting, J.P.; Braunstein, M.
The SecA2 secretion factor of Mycobacterium tuberculosis promotes growth in macrophages and inhibits the host immune response
Infect. Immun.
74
6855-6864
2006
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Gibbons, H.S.; Wolschendorf, F.; Abshire, M.; Niederweis, M.; Braunstein, M.
Identification of two Mycobacterium smegmatis lipoproteins exported by a SecA2-dependent pathway
J. Bacteriol.
189
5090-5100
2007
Mycolicibacterium smegmatis (A0QYG9), Mycolicibacterium smegmatis, Mycobacterium tuberculosis (P9WGP3), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WGP3)
Manually annotated by BRENDA team
Hou, J.M.; DLima, N.G.; Rigel, N.W.; Gibbons, H.S.; McCann, J.R.; Braunstein, M.; Teschke, C.M.
ATPase activity of Mycobacterium tuberculosis SecA1 and SecA2 proteins and its importance to SecA2 function in macrophages
J. Bacteriol.
190
4880-4887
2008
Escherichia coli (P10408), Escherichia coli, Mycobacterium tuberculosis (P9WGP3), Mycobacterium tuberculosis (P9WGP5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WGP3), Mycobacterium tuberculosis H37Rv (P9WGP5)
Manually annotated by BRENDA team
Rigel, N.W.; Gibbons, H.S.; McCann, J.R.; McDonough, J.A.; Kurtz, S.; Braunstein, M.
The Accessory SecA2 System of Mycobacteria Requires ATP Binding and the Canonical SecA1
J. Biol. Chem.
284
9927-9936
2009
Mycolicibacterium smegmatis (A0QYG9), Mycolicibacterium smegmatis (P71533), Mycolicibacterium smegmatis, Mycobacterium tuberculosis (A0QYG9), Mycobacterium tuberculosis (P71533), Mycobacterium tuberculosis
Manually annotated by BRENDA team
Feltcher, M.E.; Gunawardena, H.P.; Zulauf, K.E.; Malik, S.; Griffin, J.E.; Sassetti, C.M.; Chen, X.; Braunstein, M.
Label-free quantitative proteomics reveals a role for the Mycobacterium tuberculosis SecA2 pathway in exporting solute binding proteins and Mce transporters to the cell wall
Mol. Cell. Proteomics
14
1501-1516
2015
Mycobacterium tuberculosis (P9WGP3), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WGP3)
Manually annotated by BRENDA team
Prabudiansyah, I.; Kusters, I.; Driessen, A.J.
In vitro interaction of the housekeeping SecA1 with the accessory SecA2 protein of Mycobacterium tuberculosis
PLoS ONE
10
e0128788
2015
Mycobacterium tuberculosis (P9WGP3), Mycobacterium tuberculosis (P9WGP5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WGP3), Mycobacterium tuberculosis H37Rv (P9WGP5)
Manually annotated by BRENDA team