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Information on EC 7.4.2.5 - bacterial ABC-type protein transporter and Organism(s) Bacillus subtilis and UniProt Accession P28366

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IUBMB Comments
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. This entry stands for a family of bacterial enzymes that are dedicated to the secretion of one or several closely related proteins belonging to the toxin, protease and lipase families. Examples from Gram-negative bacteria include alpha-hemolysin, cyclolysin, colicin V and siderophores, while examples from Gram-positive bacteria include bacteriocin, subtilin, competence factor and pediocin.
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This record set is specific for:
Bacillus subtilis
UNIPROT: P28366
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
pept1, peptide transporter, pept2, seca2, abc transport, seca protein, peptide transporter 1, abcb10, seca1, seca atpase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
peptide-transporting ATPase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transmembrane transport
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hydolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (ABC-type, peptide-exporting)
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. This entry stands for a family of bacterial enzymes that are dedicated to the secretion of one or several closely related proteins belonging to the toxin, protease and lipase families. Examples from Gram-negative bacteria include alpha-hemolysin, cyclolysin, colicin V and siderophores, while examples from Gram-positive bacteria include bacteriocin, subtilin, competence factor and pediocin.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + subtilisin/in
ADP + phosphate + subtilisin/out
show the reaction diagram
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + subtilisin/in
ADP + phosphate + subtilisin/out
show the reaction diagram
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-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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SpaF, ATP-binding protein, membrane spanning domain located at C-terminus
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22300
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SpaG, nucleotide sequence
50200
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SpaF, nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
X-ray diffraction reveals that the dimeric SecA ATPase dissociates in monomers during protein translocation
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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large in-frame deletion of SpaT, not translocation is interrupted, but proteolytic cleavage of the leader segment while the precurser resides in the cell wall
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain Bl21DE3 as a recombinant C-terminal His6-tagged protein
subtilin gene
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Klein, C.; Entian, K.D.
Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633
Appl. Environ. Microbiol.
60
2793-2801
1994
Bacillus subtilis
Manually annotated by BRENDA team
Izaguirre, G.; Hansen, J.N.
Use of alkaline phosphatase as a reporter polypeptide to study the role of the subtilin leader segment and the SpaT transporter in the posttranslational modifications and secretion of subtilin in Bacillus subtilis 168
Appl. Environ. Microbiol.
63
3965-3971
1997
Bacillus subtilis, Bacillus subtilis 168
Manually annotated by BRENDA team
Chung, Y.J.; Steen, M.T.; Hansen, J.N.
The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport protein
J. Bacteriol.
174
1417-1422
1992
Bacillus subtilis
Manually annotated by BRENDA team
Zimmer, J.; Nam, Y.; Rapoport, T.A.
Structure of a complex of the ATPase SecA and the protein-translocation channel
Nature
455
936-943
2008
Thermotoga maritima, Bacillus subtilis (P28366)
Manually annotated by BRENDA team