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ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
ATP + H2O + Cd2+/out
ADP + phosphate + Cd2+/in
-
-
-
-
?
ATP + H2O + Pb2+/in
ADP + phosphate + Pb2+/out
-
-
-
?
ATP + H2O + Zn2+/in
ADP + phosphate + Zn2+/out
additional information
?
-
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
complementation experiments suggest that Pb2+ and Zn2+ are not transported by the enzyme
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
complementation experiments suggest that Pb2+, Zn2+, Co2+ and Ni2+ are not transported by the enzyme
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
the N-terminus of Pca1 contains a metal-responding degradation signal encompassing amino acids 250-350. Seven cysteine residues within the Pca1 regulatory domain are required for metal sensing
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
the N-terminus of Pca1 contains a metal-responding degradation signal encompassing amino acids 250-350. Seven cysteine residues within the Pca1 regulatory domain are required for metal sensing
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Zn2+/in
ADP + phosphate + Zn2+/out
-
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Zn2+/in
ADP + phosphate + Zn2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Zn2+/in
ADP + phosphate + Zn2+/out
-
-
-
?
additional information
?
-
involved in Cd2+, Pb2+, and Zn2+ resistance
-
-
?
additional information
?
-
involved in Cd2+, Pb2+, and Zn2+ resistance
-
-
?
additional information
?
-
-
mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway
-
-
?
additional information
?
-
-
mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway
-
-
?
additional information
?
-
involved in Cd2+, Pb2+, and Zn2+ resistance, not involved in Hg2+ resistance
-
-
?
additional information
?
-
-
cadmium and zinc lead to the upregulation of genes encoded on the pSymA or pSymB plasmids, expression of the efflux pumps SMc01095, i.e. mexF1, and SMb20345 is enhanced 41fold and 4fold, respectively, overview
-
-
?
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ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
ATP + H2O + Zn2+/in
ADP + phosphate + Zn2+/out
additional information
?
-
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Zn2+/in
ADP + phosphate + Zn2+/out
-
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
ATP + H2O + Zn2+/in
ADP + phosphate + Zn2+/out
active transport of Cd2+ out of the cell to maintain metal homeostasis
-
-
?
additional information
?
-
involved in Cd2+, Pb2+, and Zn2+ resistance
-
-
?
additional information
?
-
involved in Cd2+, Pb2+, and Zn2+ resistance
-
-
?
additional information
?
-
-
mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway
-
-
?
additional information
?
-
-
mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway
-
-
?
additional information
?
-
involved in Cd2+, Pb2+, and Zn2+ resistance, not involved in Hg2+ resistance
-
-
?
additional information
?
-
-
cadmium and zinc lead to the upregulation of genes encoded on the pSymA or pSymB plasmids, expression of the efflux pumps SMc01095, i.e. mexF1, and SMb20345 is enhanced 41fold and 4fold, respectively, overview
-
-
?
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C354A
-
transmembrane segment 6, participation in Cd2+ binding, inactive in transport of Cd2+, no ATPase activity
C356A
-
transmembrane segment 6, participation in Cd2+ binding, inactive in transport of Cd2+
D398A
-
no enzymic activity
D692A
-
transmembrane segment 8, participation in Cd2+ binding, inactive in transport of Cd2+, no ATPase activity
E164A
-
transmembrane segment 4, reduced ATPase activity
M149A
-
transmembrane segment 3, participation in Cd2+ binding, reduced ATPase activity
P355A
-
transmembrane segment 6, reduced ATPase activity
C298A/C300A
-
site-directed mutation of the CXC motif abolishes copper resistance but not cadmium resistance
C311A/C312A
-
site-directed mutation of the CC motif abolishes cadmium resistance yet retains the ability to confer copper resistance
G970R
-
naturally occurring missense mutation presented in a number of yeast laboratory strains, loss of function, mutation is not found in wild type strains
C298A/C300A
-
site-directed mutation of the CXC motif abolishes copper resistance but not cadmium resistance
-
C311A/C312A
-
site-directed mutation of the CC motif abolishes cadmium resistance yet retains the ability to confer copper resistance
-
additional information
construction of a GFP-fussion protein
additional information
construction of a C-terminal fragment that contains heavy metal-binding domains
additional information
-
site-directed mutagenesis of conserved amino acids among P1B-type ATPases, including the CXXC motif (C421, C424), the CPX motif (C859), and the phosphorylation site (Asp-903), completely abolished cadmium resistance
additional information
-
deletion of the N-terminal extension domain results in constitutive expression of Pca1 at the cell surface even in the absence of cadmium. Pca1 is not stabilized in a yeast strain defective in endocytosis, GFP-Pca1 does not accumulate in the vacuole of the DELTApep4 strain
additional information
-
deletion of the N-terminal extension domain results in constitutive expression of Pca1 at the cell surface even in the absence of cadmium. Pca1 is not stabilized in a yeast strain defective in endocytosis, GFP-Pca1 does not accumulate in the vacuole of the DELTApep4 strain
-
additional information
-
construction of mutant strains containing mini-Tn5 transposons, analysis of mutation effects on protein expression levels in presence of high concentrations of cadmium or zinc
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Silver, S.; Nucifora, G.; Phung, L.T.
Human Menkes X-chromosome disease and the staphylococcal cadmium-resistance ATPase: a remarkable similarity in protein sequences
Mol. Microbiol.
10
7-12
1993
Staphylococcus aureus
brenda
Lebrun, M.; Audurier, A.; Cossart, P.
Plasmid-borne cadmium resistance genes in Listeria monocytogenes are similar to cadA and cadC of Staphylococcus aureus and are induced by cadmium
J. Bacteriol.
176
3040-3048
1994
Listeria monocytogenes
brenda
Tsai, K.J.; Yoon, K.P.; Lynn, A.R.
ATP-dependent cadmium transport by the cadA cadmium resistance determinat in everted membrane vesicles of Bacillus subtilis
J. Bacteriol.
174
116-121
1992
Bacillus subtilis
brenda
Nucifora, G.; Chu, L.; Misra, T.K.; Silver, S.
Cadmium resistance from Staphylococcus aureus plasmid pI258 cadA gene results from a cadmium-efflux ATPase
Proc. Natl. Acad. Sci. USA
86
3544-3548
1989
Staphylococcus aureus
brenda
Rensing, C.; Sun, Y.; Mitra, B.; Rosen, B.P.
Pb(II)-translocating P-type ATPase
J. Biol. Chem.
273
32614-32617
1998
Staphylococcus aureus
brenda
Lee, S.W.; Glickmann, E.; Cooksey, D.A.
Chromosomal locus for cadmium resistance in Pseudomonas putida consisting of a cadmium-transporting ATPase and a MerR family response regulator
Appl. Environ. Microbiol.
67
1437-1444
2001
Pseudomonas putida (Q93TP6), Pseudomonas putida 6909 (Q93TP6)
brenda
Bal, N.; Wu, C.C.; Catty, P.; Guillain, F.; Mintz, E.
Cd2+ and the N-terminal metal-binding domain protect the putative membranous CPC motif of the Cd2+-ATPase of Listeria monocytogenes
Biochem. J.
369
681-685
2003
Listeria monocytogenes
brenda
Gaballa, A.; Helmann, J.D.
Bacillus subtilis CPx-type ATPases: Characterization of Cd, Zn, Co and Cu efflux systems
BioMetals
16
497-505
2003
Bacillus subtilis, Bacillus subtilis 168 / CU1065
brenda
Gravot, A.; Lieutaud, A.; Verret, F.; Auroy, P.; Vavasseur, A.; Richaud, P.
AtHMA3, a plant P1B-ATPase, functions as a Cd/Pb transporter in yeast
FEBS Lett.
561
22-28
2004
Arabidopsis thaliana (P0CW78)
brenda
Bernard, C.; Roosens, N.; Czernic, P.; Lebrun, M.; Verbruggen, N.
A novel CPx-ATPase from the cadmium hyperaccumulator Thlaspi caerulescens
FEBS Lett.
569
140-148
2004
Noccaea caerulescens (Q70LF4)
brenda
Papoyan, A.; Kochian, L.V.
Identification of Thlaspi caerulescens genes that may be involved in heavy metal hyperaccumulation and tolerance - characterization of a novel heavy metal transporting ATPase
Plant Physiol.
136
3814-3823
2004
Noccaea caerulescens (Q69AX6)
brenda
Wu, C.C.; Bal, N.; Perard, J.; Lowe, J.; Boscheron, C.; Mintz, E.; Catty, P.
A cloned prokaryotic Cd2+ P-type ATPase increases yeast sensitivity to Cd2+
Biochem. Biophys. Res. Commun.
324
1034-1040
2004
Listeria monocytogenes
brenda
Wu, C.C.; Gardarin, A.; Catty, P.; Guillain, F.; Mintz, E.
CadA, the Cd(2+)-ATPase from Listeria monocytogenes, can use Cd(2+) as co-substrate
Biochimie
88
1687-1692
2006
Listeria monocytogenes
brenda
Wu, C.C.; Gardarin, A.; Martel, A.; Mintz, E.; Guillain, F.; Catty, P.
The cadmium transport sites of CadA, the Cd(2+)-ATPase from Listeria monocytogenes
J. Biol. Chem.
281
29533-29541
2006
Listeria monocytogenes
brenda
Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.C.; Miras, R.; Bal, N.; Mintz, E.; Catty, P.; Shokes, J.E.; Scott, R.A.
Structural basis for metal binding specificity: the N-terminal cadmium binding domain of the P1-type ATPase CadA
J. Mol. Biol.
356
638-650
2006
Escherichia coli, Listeria monocytogenes (Q60048)
brenda
Perez, J.M.; Pradenas, G.A.; Navarro, C.A.; Henriquez, D.R.; Pichuantes, S.E.; Vasquez, C.C.
Geobacillus stearothermophilus LV cadA gene mediates resistance to cadmium, lead and zinc in zntA mutants of Salmonella enterica serovar Typhimurium
Biol. Res.
39
661-668
2006
Salmonella enterica (Q8ZLE5), Geobacillus stearothermophilus (Q93GJ9), Geobacillus stearothermophilus LV (Q93GJ9)
brenda
Adle, D.J.; Sinani, D.; Kim, H.; Lee, J.
A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae
J. Biol. Chem.
282
947-955
2007
Saccharomyces cerevisiae
brenda
Rossbach, S.; Mai, D.J.; Carter, E.L.; Sauviac, L.; Capela, D.; Bruand, C.; de Bruijn, F.J.
Response of Sinorhizobium meliloti to elevated concentrations of cadmium and zinc
Appl. Environ. Microbiol.
74
4218-4221
2008
Sinorhizobium meliloti
brenda
Adle, D.J.; Lee, J.
Expressional control of a cadmium-transporting P1B-type ATPase by a metal sensing degradation signal
J. Biol. Chem.
283
31460-31468
2008
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
brenda
Kwong, R.W.; Niyogi, S.
Cadmium transport in isolated enterocytes of freshwater rainbow trout: interactions with zinc and iron, effects of complexation with cysteine, and an ATPase-coupled efflux
Comp. Biochem. Physiol. C
155
238-246
2012
Oncorhynchus mykiss
brenda
Migocka, M.; Papierniak, A.; Kosatka, E.; Klobus, G.
Comparative study of the active cadmium efflux systems operating at the plasma membrane and tonoplast of cucumber root cells
J. Exp. Bot.
62
4903-4916
2011
Cucumis sativus
brenda
Miyadate, H.; Adachi, S.; Hiraizumi, A.; Tezuka, K.; Nakazawa, N.; Kawamoto, T.; Katou, K.; Kodama, I.; Sakurai, K.; Takahashi, H.; Satoh-Nagasawa, N.; Watanabe, A.; Fujimura, T.; Akagi, H.
OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles
New Phytol.
189
190-199
2011
Oryza sativa
brenda
Chien, C.; Huang, C.; Lin, Y.
Characterization of a heavy metal translocating P-type ATPase gene from an environmental heavy metal resistance Enterobacter sp. isolate
Appl. Biochem. Biotechnol.
169
1837-1846
2013
Enterobacter sp. (M9NX10), Enterobacter sp. CD01 (M9NX10)
brenda
Liu, H.; Zhao, H.; Wu, L.; Liu, A.; Zhao, F.J.; Xu, W.
Heavy metal ATPase 3 (HMA3) confers cadmium hypertolerance on the cadmium/zinc hyperaccumulator Sedum plumbizincicola
New Phytol.
215
687-698
2017
Sedum plumbizincicola (A0A1W6EUG1), Sedum plumbizincicola
brenda
Maynaud, G.; Brunel, B.; Yashiro, E.; Mergeay, M.; Cleyet-Marel, J.C.; Le Quere, A.
CadA of Mesorhizobium metallidurans isolated from a zinc-rich mining soil is a P(IB-2)-type ATPase involved in cadmium and zinc resistance
Res. Microbiol.
165
175-189
2014
Mesorhizobium metallidurans (I4IY19), Mesorhizobium metallidurans STM 2683T (I4IY19)
brenda