This iron-sulfur and flavin-containing electron transport complex, isolated from the bacterium Acetobacterium woodii, couples the energy from reduction of NAD+ by ferredoxin to pumping sodium ions out of the cell, generating a gradient across the cytoplasmic membrane.
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SYSTEMATIC NAME
IUBMB Comments
ferredoxin:NAD+ oxidoreductase (Na+-transporting)
This iron-sulfur and flavin-containing electron transport complex, isolated from the bacterium Acetobacterium woodii, couples the energy from reduction of NAD+ by ferredoxin to pumping sodium ions out of the cell, generating a gradient across the cytoplasmic membrane.
the enzyme couples oxidation of reduced ferredoxin (generated by hydrogenase) with the reduction of NAD+. The Na+-translocating enzyme catalyzes electron transfer from ferredoxin to NAD+ coupled to electrogenic ion transport across the membrane
the enzyme couples oxidation of reduced ferredoxin (generated by hydrogenase) with the reduction of NAD+. The Na+-translocating enzyme catalyzes electron transfer from ferredoxin to NAD+ coupled to electrogenic ion transport across the membrane
the enzyme complex is inhibited by sodium ionophores but not protonophores, demonstrating a direct coupling of the ferredoxin:NAD+ oxidoreductase activity to Na+ transport
C4N8U0: electron transport complex subunit C, C4N8U1: electron transport complex subunit D, C4N8U2: electron transport complex subunit G, C4N8U3: electron transport complex subunit E, C4N8U4: electron transport complex subunit A, C4N8U5: electron transport complex subunit B
acetogens can be divided into two groups, the Na+-dependent ones with Acetobacterium woodii and the H+-dependent ones with Moorella thermoacetica (formerly Clostridium thermoaceticum) as model organisms
role of the enzyme Rnf complex in the Wood-Ljungdahl pathway, overview. Acetogens use the WoodLjungdahl pathway for reduction of carbon dioxide to acetate. This pathway not only allows reoxidation of reducing equivalents during heterotrophic growth but also supports chemolithoautotrophic growth on H2 +CO2. In addition to CO2, acetogens can use alternative electron acceptors, such as nitrate or caffeate. Caffeate respiration in the model acetogen Acetobacterium woodii is coupled to energy conservation via a chemiosmotic mechanism, with Na+ as coupling ion. Coupling of the Wood-Ljungdahl pathway to primary and electrogenic translocation of Na+ across the cytoplasmic membrane in Acetobacterium woodii
the enzyme couples oxidation of reduced ferredoxin (generated by hydrogenase) with the reduction of NAD+. The Na+-translocating enzyme catalyzes electron transfer from ferredoxin to NAD+ coupled to electrogenic ion transport across the membrane. The enzyme is also involved in the electron-transfer pathway in caffeate respiration
the Rnf complex may have a central role in the bioenergetics of Acetobacterium woodii. Oxidation of pyruvate (glycolysis) or H2 (chemolithoautotrophic growth) yields reduced ferredoxin. Electrons are wired to NAD+ by Rnf thereby generating a Na+-potential. Depending on the availability of electron acceptors, either CO2 or caffeate is reduced, overview
endergonic reduction of ferredoxin with NADH is driven by reverse electron transport catalyzed by the Rnf complex. Deletion of subunits RnfABCDEG results in a mutant that does not grow on H2 plus CO2, nor does it produce acetate or ATP from H2 plus CO2, and ferredoxin:NADx02 oxidoreductase activity and Na+ translocation are also completely lost. The mutant also does not grow on low-energy substrates, such as ethanol or lactate
the enzyme complex is composed of at least six different subunits, x * 35000 (subunit RnfD), x * 22800 (subunit RnfG), x * 21600 (subunit RnfE), x * 21400 (subunit RnfA), x * 36600 (subunit RnfB), calculated from sequence
the enzyme complex is composed of at least six different subunits, x * 35000 (subunit RnfD), x * 22800 (subunit RnfG), x * 21600 (subunit RnfE), x * 21400 (subunit RnfA), x * 36600 (subunit RnfB), calculated from sequence
the enzyme complex is composed of at least six different subunits, x * 35000 (subunit RnfD), x * 22800 (subunit RnfG), x * 21600 (subunit RnfE), x * 21400 (subunit RnfA), x * 36600 (subunit RnfB), calculated from sequence
the enzyme complex is composed of at least six different subunits, x * 35000 (subunit RnfD), x * 22800 (subunit RnfG), x * 21600 (subunit RnfE), x * 21400 (subunit RnfA), x * 36600 (subunit RnfB), calculated from sequence
the enzyme complex is composed of at least six different subunits, x * 35000 (subunit RnfD), x * 22800 (subunit RnfG), x * 21600 (subunit RnfE), x * 21400 (subunit RnfA), x * 36600 (subunit RnfB), calculated from sequence
the enzyme complex is composed of at least six different subunits, x * 35000 (subunit RnfD), x * 22800 (subunit RnfG), x * 21600 (subunit RnfE), x * 21400 (subunit RnfA), x * 36600 (subunit RnfB), calculated from sequence
Westphal, L.; Wiechmann, A.; Baker, J.; Minton, N.; Mueller, V.
The Rnf complex is an energy-coupled transhydrogenase essential to reversibly link cellular NADH and ferredoxin pools in the acetogen Acetobacterium woodii
J. Bacteriol.
200
e00357
2018
Acetobacterium woodii (H6LC28 and H6LC27 and H6LC32 and H6LC31 and H6LC29 and H6LC30), Acetobacterium woodii, Acetobacterium woodii DSM 1030 (H6LC28 and H6LC27 and H6LC32 and H6LC31 and H6LC29 and H6LC30)