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Information on EC 6.4.1.7 - 2-oxoglutarate carboxylase

for references in articles please use BRENDA:EC6.4.1.7

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EC Tree

6 Ligases

6.4 Forming carbon-carbon bonds

6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)

6.4.1.7 2-oxoglutarate carboxylase

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The enzyme appears in viruses and cellular organisms

Reaction Schemes

Synonyms

carboxylating factor for ICDH, CFI, OGC, oxalosuccinate synthetase, show all synonyms

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

carboxylating factor for ICDH

2 entries

CFI

2 entries

OGC

2 entries

oxalosuccinate synthetase

2 entries

carboxylating factor for ICDH

Hydrogenobacter thermophilus

incorrect

670306

carboxylating factor for ICDH

Hydrogenobacter thermophilus TK-6 / IAM 12695

incorrect

CFI

Hydrogenobacter thermophilus

670306

CFI

Hydrogenobacter thermophilus TK-6 / IAM 12695

OGC

Hydrogenobacter thermophilus

670306

OGC

Hydrogenobacter thermophilus TK-6 / IAM 12695

oxalosuccinate synthetase

Hydrogenobacter thermophilus

670306

oxalosuccinate synthetase

Hydrogenobacter thermophilus TK-6 / IAM 12695

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

ATP + 2-oxoglutarate + HCO3- = ADP + phosphate + oxalosuccinate

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Go to Pathway Search

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PATHWAY SOURCE

PATHWAYS

MetaCyc

reductive TCA cycle II

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

5 entries

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus

670306, 670325

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus

first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41

670325

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus TK-6 / IAM 12695

670306, 670325

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus TK-6 / IAM 12695

670325

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Rattus norvegicus

667453

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

4 entries

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus

670306

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus

670325

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus TK-6 / IAM 12695

670306

ATP + 2-oxoglutarate + HCO3-

ADP + phosphate + oxalosuccinate

Hydrogenobacter thermophilus TK-6 / IAM 12695

670325

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

biotin

Hydrogenobacter thermophilus

enzyme contains biotin

670306, 670325

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mg2+

Hydrogenobacter thermophilus

required

670306

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

Avidin

Hydrogenobacter thermophilus

670306

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.03

2-oxoglutarate

Hydrogenobacter thermophilus

pH 8.5, 20°C

670325

0.788

ATP

Hydrogenobacter thermophilus

pH 8.5, 20°C

670325

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

30.6

2-oxoglutarate

Hydrogenobacter thermophilus

pH 8.5, 20°C

670325

31.7

ATP

Hydrogenobacter thermophilus

pH 8.5, 20°C

670325

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Hydrogenobacter thermophilus

670325

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

70 - 80

Hydrogenobacter thermophilus

670325

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pI VALUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

4.9

Hydrogenobacter thermophilus

isoelectric focusing

670325

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Hydrogenobacter thermophilus

TK-6

670306, 670325

brenda

Hydrogenobacter thermophilus TK-6 / IAM 12695

TK-6

brenda

brenda

show all columns Go to Source Tissue Search

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

show all columns Go to Localization Search

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

Show AA Sequence and Transmembrane Helices (637 entries)
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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

49000

Hydrogenobacter thermophilus

x * 72000 + x * 49000, likely subunit structure alpha8beta8, SDS-PAGE

670306

72000

Hydrogenobacter thermophilus

x * 72000 + x * 49000, likely subunit structure alpha8beta8, SDS-PAGE

670306

998000

Hydrogenobacter thermophilus

gel filtration

670306

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 entries

Hydrogenobacter thermophilus

x * 72000 + x * 49000, likely subunit structure alpha8beta8, SDS-PAGE

670306

Hydrogenobacter thermophilus TK-6 / IAM 12695

x * 72000 + x * 49000, likely subunit structure alpha8beta8, SDS-PAGE

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Hydrogenobacter thermophilus

inactivation above

670325

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Go to Storage Stability Search

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

4°C, stable for at least 1 month

Hydrogenobacter thermophilus

670325

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Hydrogenobacter thermophilus

670306

recombinant

Hydrogenobacter thermophilus

670325

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

a fragment containing alpha and beta subunit genes is engineered for heterologous expression in Escherichia coli

Hydrogenobacter thermophilus

670325

expression in Escherichia coli

Hydrogenobacter thermophilus

670306

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

667453

Keech, D.B.; Mattoo, A.K.; Carabott, M.J.J.; Wallace, J.C.

The ATP-dependent reductive carboxylation of 2-oxoglutarate using cytosol from rat liver

Biochem. Biophys. Res. Commun.

712-718

1976

Rattus norvegicus

183779

brenda

670306

Aoshima, M.; Ishii, M.; Igarashi, Y.

A novel biotin protein required for reductive carboxylation of 2-oxoglutarate by isocitrate dehydrogenase in Hydrogenobacter thermophilus TK-6

Mol. Microbiol.

791-798

2004

Hydrogenobacter thermophilus, Hydrogenobacter thermophilus TK-6 / IAM 12695

14731279

brenda

670325

Aoshima, M.; Igarashi, Y.

A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6

Mol. Microbiol.

748-759

2006

Hydrogenobacter thermophilus, Hydrogenobacter thermophilus TK-6 / IAM 12695

17076668

brenda

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EXTERNAL LINKS (specific for EC-Number 6.4.1.7)

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

IUBMB Enzyme Nomenclature

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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