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Information on EC 6.3.5.3 - phosphoribosylformylglycinamidine synthase and Organism(s) Thermotoga maritima and UniProt Accession Q9X0X3

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Thermotoga maritima
UNIPROT: Q9X0X3 not found.
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The taxonomic range for the selected organisms is: Thermotoga maritima
The enzyme appears in selected viruses and cellular organisms
Synonyms
fgam synthetase, fgams, fgar-at, fgarat, orf75c, formylglycinamide ribonucleotide amidotransferase, phosphoribosylformylglycinamidine synthase, fgar amidotransferase, smpurl, tmpurl, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
FGAM
FGAM is part of the de novo purine biosynthesis subsystem, where it forms a complex with purS (TM1244) and purQ (TM1245) to form formylglycinamide ribonucleotide amidotransferase (FGAR-AT)
phosphoribosylformylglycinamidine synthase II
phosphoribosylformylglycinamidine synthase II is part of the de novo purine biosynthesis subsystem, where it forms a complex with purS (TM1244) and purQ (TM1245) to form formylglycinamide ribonucleotide amidotransferase (FGAR-AT)
2-Formamido-N-ribosylacetamide 5'-phosphate:L-glutamine amido-ligase (adenosine diphosphate)
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FGAM
FGAM is part of the de novo purine biosynthesis subsystem, where it forms a complex with purS (TM1244) and purQ (TM1245) to form formylglycinamide ribonucleotide amidotransferase (FGAR-AT)
FGAM synthase
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FGAM synthetase
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FGAMS
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FGAR amidotransferase
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FGARAT
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Formylglycinamide ribonucloetide amidotransferase
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Formylglycinamide ribotide amidotransferase
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Formylglycinamide ribotide synthetase
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phosphoribosylformylglycinamidine synthase II
phosphoribosylformylglycinamidine synthase II is part of the de novo purine biosynthesis subsystem, where it forms a complex with purS (TM1244) and purQ (TM1245) to form formylglycinamide ribonucleotide amidotransferase (FGAR-AT)
Phosphoribosylformylglycinamidine synthetase
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Phosphoribosylformylglycineamidine synthetase
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Synthetase, phosphoribosylformylglycinamide
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amination
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amide group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -
SYSTEMATIC NAME
IUBMB Comments
N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide:L-glutamine amido-ligase (ADP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9032-84-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-phosphoribosylformylglycinamide + ATP + L-glutamine + H2O
phosphoribosylformylglycinamidine + L-glutamate + ADP + phosphate
show the reaction diagram
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ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O
ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O
ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
show the reaction diagram
fourth step of the purine biosynthetic pathway
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop method, enzyme in complex with formylglycinamide ribonucleotide, mutant enzyme H72A in complex with beta,gamma-methylene adenosine 5'-triphosphate, mutant enzyme H72A in complex with ADP, enzyme in complex with formylglycinamide ribonucleotide and beta,gamma-methylene adenosine 5'-triphosphate, enzyme in complex with ATP
in complex with gene products PurQ and PurS, to 3.5 A resolution. The complex has a stoichiometry of 2:1:1 for components PurS:PurQ:PurL, respectively. The flexibility of the PurS dimer is involved in the activation of the complex and the formation of an ammonia channel
nanodroplet vapor diffusion method, crystal structure of phosphoribosylformylglycinamidine synthase II (smPurL) from Thermotoga maritima at 2.15 A resolution
crystal structure of phosphoribosylformyl-glycinamidine synthase II, PurS subunit (TM1244) at 1.90 A resolution
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H32A
no synthesis of formylglycinamidine ribonucleotide detected
H32Q
no synthesis of formylglycinamidine ribonucleotide detected
H72A
mutant enzyme displays 1/20 of the wild type activity with a strongly increased Km for formylglycinamidine ribonucleotide
H72Q
mutant enzyme displayed 1/200 of the wild type activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
wild-type and mutant enzymes expression in Escherichia coli
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Morar, M.; Anand, R.; Hoskins, A.A.; Stubbe, J.; Ealick, S.E.
Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily
Biochemistry
45
14880-14895
2006
Thermotoga maritima (Q9X0X3), Thermotoga maritima
Manually annotated by BRENDA team
Ambing, E.; Canaves, J.M.; Chiu, H.; Deacon, A.M.; DiDonato, M.; Elsliger, M.; Godzik, A.; Grittini, C.; Grzechnik, S.K.; Hale, J.; Hampton, E.; Han, G.W.; Haugen, J.; Jaroszewski, L.; Klock, H.E.; Koesema, E.; Kreusch, A.; Kuhn, P.; Lesley, S.A.; Levin, I.; Miller, M.D.; Moy, K.; Nigoghossian, E.; Paulsen, J.; Quijano, K.; Reyes, R.; Spraggon, G.; Stevens, R.C.; van den Bedem, H.; Velasquez, J.; White, A.; Wolf, G.; Xu, Q.; Hodgson, K.O.; Wooley, J.; Wilson, I. A.
Crystal structure of phosphoribosylformylglycinamidine synthase II (smPurL) from Thermotoga maritima at 2.15 A resolution
Proteins Struct. Funct. Bioinform.
63
1106-1111
2006
Thermotoga maritima (Q9X0X3), Thermotoga maritima
Manually annotated by BRENDA team
Mathews, I.I.; Krishna, S.S.; Schwarzenbacher, R.; McMullan, D.; Jaroszewski, L.; Miller, M.D.; Abdubek, P.; Agarwalla, S.; Ambing, E.; Axelrod, H.L.; Canaves, J.M.; Carlton, D.; Chiu, H.; Clayton, T.; DiDonato, M.; Duan, L.; Elsliger, M.; Grzechnik, S.K.
Crystal structure of phosphoribosylformyl-glycinamidine synthase II, PurS subunit (TM1244) from Thermotoga maritima at 1.90 A resolution
Proteins Struct. Funct. Bioinform.
65
249-254
2006
Thermotoga maritima (Q9X0X1)
Manually annotated by BRENDA team
Morar, M.; Hoskins, A.A.; Stubbe, J.; Ealick, S.E.
Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: structural insights into complex formation
Biochemistry
47
7816-7830
2008
Thermotoga maritima (Q9X0X3), Thermotoga maritima
Manually annotated by BRENDA team