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Information on EC 6.3.4.4 - adenylosuccinate synthase and Organism(s) Bacillus subtilis and UniProt Accession P29726

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EC Tree
     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.4 adenylosuccinate synthase
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This record set is specific for:
Bacillus subtilis
UNIPROT: P29726 not found.
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
adenylosuccinate synthetase, adssl1, adss1, adenylosuccinate synthase, pfadss, adss2, ampss, succino-amp synthetase, adenylosuccinate synthetase 1, as-synthetase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Adenylosuccinate synthase
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Adenylosuccinate synthetase
AMPSase
IMP--aspartate ligase
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IMP-aspartate ligase
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-
-
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Succino-AMP synthetase
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-
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Succinoadenylic kinosynthetase
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amination
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SYSTEMATIC NAME
IUBMB Comments
IMP:L-aspartate ligase (GDP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9023-57-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP + IMP + L-Asp
GDP + phosphate + adenylosuccinate
show the reaction diagram
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-
-
?
GTP + IMP + L-aspartate
GDP + phosphate + adenylosuccinate
show the reaction diagram
GTP + IMP + L-aspartate
GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + IMP + L-aspartate
GDP + phosphate + adenylosuccinate
show the reaction diagram
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first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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overview
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.68
Asp
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0.0044
GTP
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pH 7.7, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.47
GTP
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pH 7.7, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
107
GTP
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pH 7.7, 30°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0062
GDP
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pH 7.7, 30°C
0.007
ppGpp
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pH 7.7, 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P242N
about 50% of wild-type activity. Mutant partially reduces the flux towards AMP derived from IMP and increases the riboflavin synthesis precursor GTP
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
expression of mutant P242N in an inosine-producing strain results in an approximately 4.66fold increase in inosine production, to 0.41 g/L, in minimal medium without hypoxanthine accumulation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Stayton, M.M.; Rudolph, F.B.; Fromm, H.J.
Regulation, genetics, and properties of adenylosuccinate synthetase
Curr. Top. Cell. Regul.
22
103-141
1983
Azotobacter vinelandii, Bacillus subtilis, Gallus gallus, Oryctolagus cuniculus, Escherichia coli, Homo sapiens, Leishmania donovani, Rattus norvegicus, Schizosaccharomyces pombe, Sus scrofa, Triticum aestivum, Trypanosoma cruzi
Manually annotated by BRENDA team
Honzatko, R.B.; Stayton, M.M.; Fromm, H.J.
Adenylosuccinate synthetase: Recent developments
Adv. Enzymol. Relat. Areas Mol. Biol.
73
57-102
1999
Azotobacter vinelandii, Acidithiobacillus ferrooxidans, Arabidopsis thaliana, Bacillus subtilis, Oryctolagus cuniculus, Dictyostelium discoideum, Escherichia coli, Haemophilus influenzae, Homo sapiens, Leishmania donovani, Methanocaldococcus jannaschii, Mus musculus, Pyrococcus sp., Rattus norvegicus, Schizosaccharomyces pombe, Triticum aestivum, Trypanosoma cruzi, Zea mays, Pyrococcus sp. ST700
Manually annotated by BRENDA team
Nomura, Y.; Nozawa, A.; Tozawa, Y.
Biochemical analyses of ppGpp effect on adenylosuccinate synthetases, key enzymes in purine biosynthesis in rice
Biosci. Biotechnol. Biochem.
78
1022-1025
2014
Bacillus subtilis, Bacillus subtilis 168, Escherichia coli, Oryza sativa
Manually annotated by BRENDA team
Wang, X.; Wang, G.; Li, X.; Fu, J.; Chen, T.; Wang, Z.; Zhao, X.
Directed evolution of adenylosuccinate synthetase from Bacillus subtilis and its application in metabolic engineering
J. Biotechnol.
231
115-121
2016
Bacillus subtilis (P29726), Bacillus subtilis, Bacillus subtilis 168 (P29726)
Manually annotated by BRENDA team