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nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
additional information
?
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O

beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the pyridine N and carboxyl groups are important for interaction with the enzyme
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
in the presence of Mg2+ and inorganic phosphate
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
lack of the de novo pathway for NAD+ synthesis from tryptophan, utilizes both nicotinic acid and nicotinamide as NAD+ precursors
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
NAPRT is essential for nicotinic acid to increase cellular NAD levels and thus to prevent oxidative stress of the cells
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
+ whole cell lysates, pH 7.5, 37°C, 10 mM MgCl2, 2.5 mM dithiothreitol, 1 mM ATP, 25 microgram bovine serum albumin, with or without NAD+
termination by boiling for 60 seconds, analysis by thin layer chromatography (radiolabeled nicotinic acid) or ESI-MS, no NaMN detectable in Hep-G2 cells
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
essential for the NAD salvage pathway
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
mutants in the His-219 residue do catalyze the slow formation of nicotinic acid mononucleotide in the absence of ATP similarly to the wild type enzymes
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
no phosphoribosylation of nicotinamide, quinolic acid, adenine or hypoxanthine
-
-
-
additional information

?
-
-
one mol ATP is cleaved per mol product formed
-
-
-
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
-
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
-
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
-
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
-
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
-
additional information
?
-
nicotinamide is not substrate
-
-
-
additional information
?
-
quinolinic acid is not substrate
-
-
-
additional information
?
-
-
enzyme is able to generate adenosine 5'-tetraphosphate using substrate ATP and product phosphate, reaction of EC 3.6.1.14. Accompanying a typically irreversible hydrolysis of ATP to ADP and phosphate, is a fully reversible phosphate transfer reaction between ATP and adenosine 5'-tetraphosphate
-
-
-
additional information
?
-
-
-
-
-
additional information
?
-
-
ATP and phosphoribose diphosphate compete for ATP-binding site
-
-
-
additional information
?
-
-
ATPase activity in the presence of either product and in the absence of phosphoribose diphosphate
-
-
-
additional information
?
-
molecular evolution mechanism
-
-
-
additional information
?
-
-
negligible ATPase activity in the absence of nicotinic acid
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
additional information
?
-
P39683
molecular evolution mechanism
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O

beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Q6XQN6
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
lack of the de novo pathway for NAD+ synthesis from tryptophan, utilizes both nicotinic acid and nicotinamide as NAD+ precursors
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Q6XQN6
NAPRT is essential for nicotinic acid to increase cellular NAD levels and thus to prevent oxidative stress of the cells
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Q8CC86
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Q8CC86
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Q8CC86
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Q6XQN1
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
P39683
essential for the NAD salvage pathway
-
-
r
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Arthritis, Rheumatoid
Serum gliostatin levels in patients with rheumatoid factor-negative and -positive rheumatoid arthritis and changes of these levels after surgical treatments.
Breast Neoplasms
[Measurement of PyNPase and analysis on correlation between breast cancer tissue and lymph node]
Breast Neoplasms
[Prognostic effect of PyNPase (pyrimidine nucleoside phosphorylase) activity in breast cancer]
Breast Neoplasms
[PyNPase and DPD expression potentially predict response to 5'-DFUR treatment for node-positive breast cancer patients]
Breast Neoplasms
[Significance of tissue PyNPase, TS, and DPD activities in breast cancer]
Carcinoma
Dependence of tumor cell lines and patient-derived tumors on the NAD salvage pathway renders them sensitive to NAMPT inhibition with GNE-618.
Carcinoma
Pd-ECGF positivity correlates with better survival, while iNOS has no predictive value for cervical carcinomas treated with radiotherapy.
Carcinoma
PD-ECGF, bFGF, and VEGF expression in non-small cell lung carcinomas and their association with lymph node metastasis.
Carcinoma
TGF-alpha as well as VEGF, PD-ECGF and bFGF contribute to angiogenesis of esophageal squamous cell carcinoma.
Carcinoma, Hepatocellular
Effects of 5'-DFUR and lentinan on cytokines and PyNPase against AH66 ascites hepatoma in rats.
Colonic Neoplasms
PyNPase expression in human colon cancer.
Colorectal Neoplasms
[Antitumor effect of 5'-DFUR and PyNPase activity in colorectal cancer]
Colorectal Neoplasms
[PyNPase activity in primary and metastatic colorectal cancer]
Colorectal Neoplasms
[Study on the relationship between concentrations of 5-FU and PyNPase activity in tumor tissue during oral 5'-DFUR treatment in patients with advanced colorectal cancer]
Esophageal Squamous Cell Carcinoma
TGF-alpha as well as VEGF, PD-ECGF and bFGF contribute to angiogenesis of esophageal squamous cell carcinoma.
Glioblastoma
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Glioblastoma
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Glucose Intolerance
Tissue-specific regulation of sirtuin and nicotinamide adenine dinucleotide biosynthetic pathways identified in C57Bl/6 mice in response to high-fat feeding.
Lymphoma
Expression patterns of nicotinamide phosphoribosyltransferase and nicotinic acid phosphoribosyltransferase in human malignant lymphomas.
Neoplasm Metastasis
PD-ECGF, bFGF, and VEGF expression in non-small cell lung carcinomas and their association with lymph node metastasis.
Neoplasms
A preclinical study on the rescue of normal tissue by nicotinic acid in high-dose treatment with APO866, a specific nicotinamide phosphoribosyltransferase inhibitor.
Neoplasms
Dependence of tumor cell lines and patient-derived tumors on the NAD salvage pathway renders them sensitive to NAMPT inhibition with GNE-618.
Neoplasms
Dual and Specific Inhibition of NAMPT and PAK4 By KPT-9274 Decreases Kidney Cancer Growth.
Neoplasms
Effects of introduction of dThdPase cDNA on sensitivity to 5'-deoxy-5-fluorouridine and tumor angiogenesis.
Neoplasms
Extensive regulation of nicotinate phosphoribosyltransferase (NAPRT) expression in human tissues and tumors.
Neoplasms
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Neoplasms
NAMPT and NAPRT1: novel polymorphisms and distribution of variants between normal tissues and tumor samples.
Neoplasms
Nicotinic Acid Phosphoribosyltransferase Regulates Cancer Cell Metabolism, Susceptibility to NAMPT Inhibitors, and DNA Repair.
Neoplasms
Supplementation of nicotinic acid with NAMPT inhibitors results in loss of in vivo efficacy in NAPRT1-deficient tumor models.
Neoplasms
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Neoplasms
[A change of tumor PyNPase level at intraperitoneal and intravenous administrations of paclitaxel]
Neoplasms
[Correlation between pyrimidine nucleoside phosphorylase (PyNPase)/platelet-derived endothelial cell growth factor and histological prognostic factor, and influences of 5'-deoxy-5-fluorouridine (5'-DFUR) administration on PyNPase levels. 5'-DFUR Joint Research Group in the Osaka Area for Gastric Cancer]
Neoplasms
[Effect of preoperative oral 5'-DFUR on PyNPase level in gastrointestinal malignant tumor tissues]
Neoplasms
[PyNPase expression and cancer progression in the colorectum]
Neoplasms
[Study on the relationship between concentrations of 5-FU and PyNPase activity in tumor tissue during oral 5'-DFUR treatment in patients with advanced colorectal cancer]
Neoplasms
[The alteration of PyNPase activity in breast cancers due to preoperative oral administration of 5'-DFUR]
Neuroblastoma
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Neuroblastoma
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Neurofibroma
A novel glial growth inhibitory factor, gliostatin, derived from neurofibroma.
nicotinate phosphoribosyltransferase deficiency
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Ovarian Neoplasms
Nicotinic Acid Phosphoribosyltransferase Regulates Cancer Cell Metabolism, Susceptibility to NAMPT Inhibitors, and DNA Repair.
Sarcoma
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Sarcoma
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Stomach Neoplasms
[Correlation between pyrimidine nucleoside phosphorylase (PyNPase)/platelet-derived endothelial cell growth factor and histological prognostic factor, and influences of 5'-deoxy-5-fluorouridine (5'-DFUR) administration on PyNPase levels. 5'-DFUR Joint Research Group in the Osaka Area for Gastric Cancer]
Urinary Bladder Neoplasms
IFN gamma-induced up-regulation of PD-ECGF/TP enhances the cytotoxicity of 5-fluorouracil and 5'-deoxy-5-fluorouridine in bladder cancer cells.
Urinary Bladder Neoplasms
[Enhancement effect of interferon gamma on the sensitivity of RT4 bladder cancer cells to 5'-deoxy-5-fluorouridine,and 5-fluorouracil through up-regulation of PD-ECGF/TP]
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00022 - 48
5-phospho-alpha-D-ribose 1-diphosphate
1.16
ITP
-
good alternative substrate for ATP
0.02
Mg-5-phospho-alpha-D-ribose 1-diphosphate
-
in the coupled reaction, 200fold lower than in the uncoupled reaction
0.0127 - 0.0155
nicotinic acid
0.00022
5-phospho-alpha-D-ribose 1-diphosphate

-
+/-0.07 microM, in presence of 1 mM NAD+, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification
0.00025
5-phospho-alpha-D-ribose 1-diphosphate
-
+/-0.06 microM, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification
0.013
5-phospho-alpha-D-ribose 1-diphosphate
-
reduced to 0.002 mM in the presence of ATP
0.013
5-phospho-alpha-D-ribose 1-diphosphate
-
reduced to 0.002 mM in the presence of ATP
0.022
5-phospho-alpha-D-ribose 1-diphosphate
-
pncB gene, in the coupled reaction
0.0221
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C
0.024
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.03
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.0382
5-phospho-alpha-D-ribose 1-diphosphate
-
in the presence of phosphate, pH 7.5, 37°C
0.05
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.06 - 0.1
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.06 - 0.1
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.1
5-phospho-alpha-D-ribose 1-diphosphate
-
0.005 mM in the presence of ATP
2.3 - 48
5-phospho-alpha-D-ribose 1-diphosphate
-
mutant enzymes
4.5
5-phospho-alpha-D-ribose 1-diphosphate
-
-
4.5
5-phospho-alpha-D-ribose 1-diphosphate
-
pncB gene, in the uncoupled reaction
0.07
ATP

-
-
0.0005
nicotinate

-
in the presence of ATP
0.0008 - 0.0015
nicotinate
-
-
0.0015
nicotinate
-
pncB gene, in the coupled reaction
0.024
nicotinate
-
reduced to 0.0005 mM in the presence of ATP
0.0273
nicotinate
-
in the presence of phosphate, pH 7.5, 37°C
0.0443
nicotinate
-
pH 7.5, 37°C
0.22
nicotinate
-
reduced to 0.053 mM in the presence of ATP
0.22
nicotinate
-
reduced to 0.053 mM in the presence of ATP
0.29
nicotinate
-
pncB gene, in the uncoupled reaction
0.33 - 0.4
nicotinate
-
mutant enzymes
0.0127
nicotinic acid

-
+/-3.6 microM, in presence of 1 mM NAD+, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification
0.0155
nicotinic acid
-
+/-3.4 microM, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification
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