Information on EC 6.3.4.20 - 7-cyano-7-deazaguanine synthase

for references in articles please use BRENDA:EC6.3.4.20
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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
6.3.4.20
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RECOMMENDED NAME
GeneOntology No.
7-cyano-7-deazaguanine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
preQ0 biosynthesis
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tetrahydrofolate metabolism
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Folate biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
7-carboxy-7-carbaguanine:ammonia ligase (ADP-forming)
Binds Zn2+. The reaction is part of the biosynthesis pathway of queuosine.
CAS REGISTRY NUMBER
COMMENTARY hide
1256460-80-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
gene queC, renamed from ybaX
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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the enzyme belongs to GAT-QueC, a two-domain family with an N-terminal glutamine amidotransferase class-II domain fused to a domain homologous to QueC, the enzyme that produces preQ0. Phylogenetic distribution of aTGT, ArcS, GAT-QueC, and QueF-like in the two archaeal phyla, overview. Most crenarchaeal genomes encode a fused GAT-QueC protein or a QueF-like protein
malfunction
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the expression of QueC from a plasmid-borne copy confers a Q+ phenotype to enzyme-deficient mutant strain Escherichia coli B105
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-carboxy-7-carbaguanine + NH3 + ATP
7-cyano-7-carbaguanine + ADP + phosphate + H2O
show the reaction diagram
7-carboxy-7-carbaguanine + NH3 + ATP
7-cyano-7-carbaguanine + AMP + diphosphate + H2O
show the reaction diagram
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7-carboxy-7-carbaguanine + NH3 + ATP
7-cyano-7-carbaguanine + ADP + phosphate + H2O
show the reaction diagram
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?
7-carboxy-7-carbaguanine + NH3 + ATP
7-cyano-7-carbaguanine + AMP + diphosphate + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer or tetramer
the biologically relevant unit is likely to be a QueC homodimer, crystal structure. Monomer structure, overview. The Rossmann fold of the N-terminal part of QueC, in combination with the C-terminal zinc-binding motif, forms a cavity in QueC of substantial size, which is likely to be the location of substrate binding
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified QueC, vapor diffusion method, 10 mg/ml protein is mixed with crystallization solution containing 25% PEG 6000, 100 mM NaCl, 10 mM 1,6 hexanediol, and 100 mM MES, pH 5.5, 1 week, X-ray diffraction structure determination and analysis at 2.95 A resolution
molecular modeling of QueC-AMP complex. Residues Q39, T119, R97, and N98 are most likely involved in ATP binding. CDG binding likely involves residues Y129 or Y187 for pi-stacking of the CDG substrate, and D125 or D131 in binding the primary and secondary amines of the substrate pyrimidine
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
82
melting temperature, in the presence of ATP and substrate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged QueC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant QueC from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and another different step of anion exchange chromatography, followed by gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of His-tagged QueC and expression in Escherichia coli strain BL21(DE3)
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expression in Escherichia coli strain BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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