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Information on EC 6.3.4.13 - phosphoribosylamine-glycine ligase and Organism(s) Homo sapiens and UniProt Accession P22102

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EC Tree
     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.13 phosphoribosylamine-glycine ligase
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: P22102 not found.
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Word Map
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  • 6.3.4.13
  • transformylase
  • aminoimidazole
  • formyltransferase
  • amidotransferase
  • phosphoribosylaminoimidazole
  • phosphoribosylpyrophosphate
  • medicine
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
glycinamide ribonucleotide synthetase, gar synthetase, gar-syn, phosphoribosylglycinamide synthetase, phosphoribosylamine-glycine ligase, glycinamide ribotide synthetase, phosphoribosylglycineamide synthetase, phosphoribosyl-glycinamide synthetase, 5'-phosphoribosylglycinamide synthetase, prg synthetase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trifunctional purine biosynthetic protein adenosine-3
-
2-Amino-N-ribosylacetamide 5'-phosphate kinosynthase
-
-
-
-
5'-Phosphoribosylglycinamide synthetase
-
-
-
-
GAR synthetase
-
-
-
-
GARS
-
-
-
-
GARSase
-
-
-
-
Glycinamide ribonucleotide synthetase
-
-
-
-
Glycineamide ribonucleotide synthetase
-
-
-
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Phosphoribosylglycinamide synthetase
-
-
-
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Phosphoribosylglycineamide synthetase
-
-
-
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PRG synthetase
-
-
-
-
Synthetase, phosphoribosylglycinamide
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amination
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
5-phospho-D-ribosylamine:glycine ligase (ADP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9032-01-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5-phospho-D-ribosylamine + Gly
ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
-
-
-
?
ATP + 5-phosphoribosylamine + Gly
ADP + phosphate + 5-phosphoribosylglycinamide
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
second step in de novo purine biosynthesis pathway
-
?
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.228
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme displays phosphoribosylamine-glycine ligase activity, phosphoribosylformylglycinamidine cyclo-ligase activity and phosphoribosylglycinamide formyltransferase activity, cf. EC 2.1.2.2
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PUR2_HUMAN
1010
0
107767
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
112000
-
x * 112000, enzyme protein with glycinamide phosphoribosylamine-glycine ligase and aminoimidazole ribonucleotide synthetase activity, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 112000, enzyme protein with glycinamide phosphoribosylamine-glycine ligase and aminoimidazole ribonucleotide synthetase activity, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
copurification of phosphoribosylamine-glycine ligase and aminoimidazole ribonucleotide synthetase
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpressed in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
high GART expression is observed in glioma and is related to the grade of malignancy. GART overexpression is significantly associated with overall survival. Transfecting cells with GART-siRNA suppresses proliferation and enhances temozolomide-induced apoptosis in glioma cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Daubner, S.C.; Young, M.; Sammons, R.D.; Courtney, L.F.; Benkovic, S.J.
Structural and mechanistic studies on the HeLa and chicken liver proteins that catalyze glycinamide ribonucleotide synthesis and formylation and aminoimidazole ribonucleotide synthesis
Biochemistry
25
2951-2957
1986
Gallus gallus, Homo sapiens
Manually annotated by BRENDA team
Gnirke, A.; Barnes, T.S.; Patterson, D.; Schild, D.; Featherstone, T.; Olson, M.V.
Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes
EMBO J.
10
1629-1634
1991
Homo sapiens
Manually annotated by BRENDA team
Poch, M.T.; Qin, W.; Caperelli, C.A.
The human trifunctional enzyme of de novo purine biosynthesis: heterologous expression, purification, and preliminary characterization
Protein Expr. Purif.
12
17-24
1998
Homo sapiens
Manually annotated by BRENDA team
Kanai, S.; Toh, H.
Identification of new members of the GS ADP-forming family from the de novo purine biosynthesis pathway
J. Mol. Evol.
48
482-492
1999
Synechocystis sp., Arabidopsis thaliana, Archaeoglobus fulgidus, Bacillus subtilis, Saccharomyces cerevisiae, Yarrowia lipolytica, Gallus gallus, Drosophila melanogaster, Drosophila pseudoobscura, Escherichia coli, Haemophilus influenzae, Homo sapiens, Methanothermobacter thermautotrophicus, Methanocaldococcus jannaschii, Mus musculus, Pyrococcus horikoshii, Salmonella enterica subsp. enterica serovar Typhimurium, Schizosaccharomyces pombe, Chironomus tentans, Pyrococcus horikoshii OT-3
Manually annotated by BRENDA team
Liu, X.; Ding, Z.; Liu, Y.; Zhang, J.; Liu, F.; Wang, X.; He, X.; Cui, G.; Wang, D.
Glycinamide ribonucleotide formyl transferase is frequently overexpressed in glioma and critically regulates the proliferation of glioma cells
Pathol. Res. Pract.
210
256-263
2014
Homo sapiens (P22102)
Manually annotated by BRENDA team