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ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
ADP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
CDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
GDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
IDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
TDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
UTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
UDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
?
-
penultimate enzyme of biotin biosynthesis
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
ATP + diaminobiotin + CO2
ADP + phosphate + biotin
CTP + 7,8-diaminononanoate + CO2
CDP + phosphate + dethiobiotin
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
CO2 in form of HCO3-
-
-
?
ATP + diaminobiotin + CO2
ADP + phosphate + biotin
-
-
-
?
ATP + diaminobiotin + CO2
ADP + phosphate + biotin
-
37% of the activity relative to 7,8-diaminononanoate
-
-
?
CTP + 7,8-diaminononanoate + CO2
CDP + phosphate + dethiobiotin
-
-
-
-
?
CTP + 7,8-diaminononanoate + CO2
CDP + phosphate + dethiobiotin
-
50% of the activity relative to ATP
-
-
?
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in complex with CTP, to 2.3 A resolution. The nucleoside base is stabilized in its pocket through hydrogen-bonding interactions with the protein backbone, rather than amino acid side chains. DTBS could utilise ATP, CTP, GTP, ITP, TTP, or UTP with similar Km and kcat values
crystallographic studies of complexes with substrates and a reaction intermediate
-
crystals are grown from ammonium sulfate, crystals are soaked in the crystallisation-well solution plus 50 mM 6-hydroxypyrimidin-4(3H)-one for 1 hour, 6-hydroxypyrimidin-4(3H)-one is embedded in the base binding pocket of DTBS
-
hanging-drop vapor diffusion, 0.005 ml of well solution containing 100 mM magnesium acetate or MgCl2, 9-11% polyethylene glycol 8000 and 100 mM cacodylate, pH 6.5 are mixed with 0.002 ml protein solution containing 30 mg/ml DTBS, crystals grow within a week at 20°C, crystals of DTBS complexed with diaminopelargonic acid-MgADP-AlF3 and with dethiobiotin-MgADP-phosphate, crystals diffract to 1.8 A
-
hanging-drop vapor diffusion, precipitant polyethylene glycol 8000, 100 mM cacodylate, pH 6.5, 200 mM magnesium acetate, crystals diffract to 0.97 A
-
X-ray crystallographic studies of the mutant enzymes S41A, S41C, K37Q, and K37L, show that the crystals are essentially isomorphous to that of the wild-type DTBS
-
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Characterization of biotin biosynthetic enzymes of Bacillus sphaericus: a dethiobiotin producing bacterium
Agric. Biol. Chem.
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Dethiobiotin synthetase
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348-352
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Escherichia coli
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The purification and properties of dethiobiotin synthetase
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6558-6566
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Partial purification and properties of D-desthiobiotin synthetase from Escherichia coli
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Sequence and crystallization of Escherichia coli dethiobiotin synthetase, the penultimate enzyme of biotin biosynthesis
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235
774-776
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Escherichia coli
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Dethiobiotin synthetase: the carbonylation of 7,8-diaminononanoic acid proceeds regiospecifically via the N7-carbamate
Biochemistry
34
10976-10984
1995
Escherichia coli
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Structure of ATP-dependent carboxylase, dethiobiotin synthase
Methods Enzymol.
279
376-385
1997
Escherichia coli
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Substrate binding and carboxylation by dethiobiotin synthetase - a kinetic and X-ray study
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3
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1995
Escherichia coli
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Crystal structure of an ATP-dependent carboxylase, dethiobiotin synthetase, at 1.65 A resolution
Structure
2
407-414
1994
Escherichia coli
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Baxter, R.L.; Baxter, H.C.
The mechanism of Escherichia coli dethiobiotin synthetase - a closure of the ureido ring of dethiobiotin involves formation of a carbamic-phosphate mixed anhydride
J. Chem. Soc. Chem. Commun.
1994
759-760
1994
Escherichia coli
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Mechanism of dethiobiotin synthetase - characterization of the 8-aminocarbamate of (7R,8S)-7,8-diaminononanoate as an enzyme-bound intermediate
J. Chem. Soc. Chem. Commun.
1994
559-560
1994
Escherichia coli
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Active site mutants of the Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties
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36
4751-4760
1997
Escherichia coli
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Mechanism of an ATP-dependent carboxylase, dethiobiotin synthetase, based on crystallographic studies of complexes with substrates and a reaction intermediate
Biochemistry
34
10985-10995
1995
Escherichia coli
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Structure of dethiobiotin synthetase at 0.97 A resolution
Acta Crystallogr. Sect. D
55
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1999
Escherichia coli
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Crystal structure of two quaternary complexes of dethiobiotin synthetase, enzyme-MgADP-AlF3-diaminopelargonic acid and enzyme-MgADP-dethiobiotin-phosphate; implications for catalysis
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7
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Escherichia coli
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Rational design of an inhibitor of dethiobiotin synthetase; interaction of 6-hydroxypyrimidin-4(3H)-one with the adenine base binding site
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1998
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Nucleotide triphosphate promiscuity in Mycobacterium tuberculosis dethiobiotin synthetase
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