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Information on EC 6.3.3.3 - dethiobiotin synthase
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6.3.3.3 dethiobiotin synthaseIUBMB Comments
CTP has half the activity of ATP.
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Word Map
- 6.3.3.3
-
7,8-diaminopelargonic
- aminotransferase
-
dapas
-
penultimate
-
ureido
- carbamate
-
diaminopelargonic
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
dethiobiotin synthetase, dethiobiotin synthase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
BioD
Desthiobiotin synthetase
-
-
-
-
Dethiobiotin synthase
-
-
-
-
Dethiobiotin synthetase
DTB synthetase
-
-
-
-
DTBS
Synthetase, dethiobiotin
-
-
-
-
Dethiobiotin synthetase
-
-
-
-
DTBS
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
the final intermediate is the phosphoric acid anhydride of the carbamate
-
ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
mechanistic model in which binding of Mg2+ and the triphosphate element of ATP to the enzyme creates the appropriate geometry for substrate 7,8-diaminononanoate-binding and carbamate formation
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ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
8-aminocarbamate of (7R,8S)-7,8 diaminononanoate is an enzyme-bound intermediate
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ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
the carbonylation of 7,8-diaminononanoic acid proceeds regiospecifically via the N7-carbamate
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ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
enzyme reaction involves three steps: carbamylation of theN7 position of 7,8-diaminononanoate, formation of a carbamate-phosphoric anhydride intermediate, and, closure of the ureido ring to form dethiobiotin and release of the inorganic phosphate
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ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
carboxylation
-
-
-
-
heteroatomic ring closure
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
7,8-diaminononanoate:carbon-dioxide cyclo-ligase (ADP-forming)
CTP has half the activity of ATP.
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CAS REGISTRY NUMBER
COMMENTARY
37259-75-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
ADP + phosphate + dethiobiotin + Na+ + OH-
ATP + 7,8-diaminononanoate + CO2
?
-
penultimate enzyme of biotin biosynthesis
-
-
?
CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
CDP + phosphate + dethiobiotin + Na+ + OH-
GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
GDP + phosphate + dethiobiotin + Na+ + OH-
GTP + 7,8-diaminononanoate + CO2
GDP + phosphate + dethiobiotin
Pseudomonas graveolens
-
-
-
-
?
ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
IDP + phosphate + dethiobiotin + Na+ + OH-
TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
TDP + phosphate + dethiobiotin + Na+ + OH-
UTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
UDP + phosphate + dethiobiotin + Na+ + OH-
ADP + 7,8-diaminononanoate + CO2
AMP + phosphate + dethiobiotin
-
5% of the activity relative to ATP
-
-
?
ADP + 7,8-diaminononanoate + CO2
AMP + phosphate + dethiobiotin
-
5% of the activity relative to ATP
-
-
?
ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
ADP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
ADP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
ADP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
third step in the biosynthesis of biotin, overview
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
CO2 in form of HCO3-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
CO2 in form of HCO3-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
CO2 in form of HCO3-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
Pseudomonas graveolens
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
Pseudomonas graveolens
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
Saccharomyces kloeckerianus
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
Sporobolomyces coprophilus
-
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
-
-
?
ATP + diaminobiotin + CO2
ADP + phosphate + biotin
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37% of the activity relative to 7,8-diaminononanoate
-
-
?
CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
CDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
CDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
CDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
CTP + 7,8-diaminononanoate + CO2
CDP + phosphate + dethiobiotin
-
50% of the activity relative to ATP
-
-
?
CTP + 7,8-diaminononanoate + CO2
CDP + phosphate + dethiobiotin
Pseudomonas graveolens
-
-
-
-
?
GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
GDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
GDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
GDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
IDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
IDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
IDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
ITP + 7,8-diaminononanoate + CO2
IDP + phosphate + dethiobiotin
-
8.6% of the activity relative to ATP
-
-
?
ITP + 7,8-diaminononanoate + CO2
IDP + phosphate + dethiobiotin
-
8.6% of the activity relative to ATP
-
-
?
ITP + 7,8-diaminononanoate + CO2
IDP + phosphate + dethiobiotin
Pseudomonas graveolens
-
-
-
-
?
TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
TDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
TDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
TDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
UTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
UDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
UTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3
UDP + phosphate + dethiobiotin + Na+ + OH-
-
-
-
?
UTP + 7,8-diaminononanoate + CO2
UDP + phosphate + dethiobiotin
-
7% of the activity relative to ATP
-
-
?
UTP + 7,8-diaminononanoate + CO2
UDP + phosphate + dethiobiotin
-
7% of the activity relative to ATP
-
-
?
UTP + 7,8-diaminononanoate + CO2
UDP + phosphate + dethiobiotin
Pseudomonas graveolens
-
-
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 7,8-diaminononanoate + CO2
?
-
penultimate enzyme of biotin biosynthesis
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
third step in the biosynthesis of biotin, overview
-
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
ATP + 7,8-diaminononanoate + CO2
ADP + phosphate + dethiobiotin
-
penultimate step in biotin biosynthesis
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Tuberculosis
Inhibition of Mycobacterium tuberculosis Dethiobiotin Synthase (MtDTBS): Toward Next-Generation Antituberculosis Agents.
Tuberculosis
Nucleotide triphosphate promiscuity in Mycobacterium tuberculosis dethiobiotin synthetase.
Tuberculosis
Precipitant-ligand exchange technique reveals the ADP binding mode in Mycobacterium tuberculosis dethiobiotin synthetase.
Tuberculosis
Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.015
(7R,8S)-7,8-diaminopelargonic acid
mutant G169N, pH 7.5, 37°C
0.03
(7R,8S)-7,8-diaminopelargonic acid
wild-type, pH 7.5, 37°C
0.002
7,8-diaminononanoate
-
pH 7.5, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.9
(7R,8S)-7,8-diaminopelargonic acid
mutant G169N, pH 7.5, 37°C
13.3
(7R,8S)-7,8-diaminopelargonic acid
wild-type, pH 7.5, 37°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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