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EC Tree
The expected taxonomic range for this enzyme is: Psychrobacter arcticus
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Mpl
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-N-acetylmuramate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
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L-alanyl-gamma-D-glutamyl-meso-diaminopimelate:UDP-N-acetylmuramate ligase (ADP-forming)
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ATP + L-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
ATP + L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-L-lysyl-D-alanine
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
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additional information
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ATP + L-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
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ATP + L-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
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ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
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ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
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ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
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ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
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additional information
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substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala
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additional information
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substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala
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additional information
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substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
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0.0117
L-alanine
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.023
L-alanyl-gamma-D-glutamate
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.0042
L-alanyl-gamma-D-glutamyl-L-lysine
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.0017
L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
4.33
L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.098
L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.088
L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
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gene mpl
UniProt
brenda
gene mpl
UniProt
brenda
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brenda
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brenda
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evolution
Mpl and MurC-F enzymes have three domains that are classified in Pfam families, PF01225 Mur ligase catalytic domain, PF08245 Mur ligase middle domain, and PF02875 Mur ligase glutamate binding/C-terminal domain, structure comparisons, overview
evolution
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Mpl and MurC-F enzymes have three domains that are classified in Pfam families, PF01225 Mur ligase catalytic domain, PF08245 Mur ligase middle domain, and PF02875 Mur ligase glutamate binding/C-terminal domain, structure comparisons, overview
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physiological function
in Gram-negative bacteria, 30-60% of the bacterial cell wall is recycled during each generation. Part of this recycling process involves the murein peptide ligase, which attaches the breakdown product, the tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate, to UDP-MurNAc
physiological function
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in Gram-negative bacteria, 30-60% of the bacterial cell wall is recycled during each generation. Part of this recycling process involves the murein peptide ligase, which attaches the breakdown product, the tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate, to UDP-MurNAc
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additional information
unique sequence-structure relationships of Mpl proteins, overview, Residues Arg357, Arg358, Phe374, Ala375, His376, His377, Glu402, Pro403, Arg404, Ser405, Asn406, Thr407, Ser483, Asn484 and Gly485 may be important in binding tri, tetra, and pentapeptide substrates
additional information
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unique sequence-structure relationships of Mpl proteins, overview, Residues Arg357, Arg358, Phe374, Ala375, His376, His377, Glu402, Pro403, Arg404, Ser405, Asn406, Thr407, Ser483, Asn484 and Gly485 may be important in binding tri, tetra, and pentapeptide substrates
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Q4FVQ2_PSYA2
Psychrobacter arcticus (strain DSM 17307 / VKM B-2377 / 273-4)
505
0
54888
TrEMBL
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57000
x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
57187
x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
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x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
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x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
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additional information
PaMpl can be divided into 3 distinct domains: the N-terminal UDPMurNAc-binding domain (residues 1-102), the middle ATP-binding domain (residues 103-357) and the C-terminal tripeptide-binding domain (residues 358-505)
additional information
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PaMpl can be divided into 3 distinct domains: the N-terminal UDPMurNAc-binding domain (residues 1-102), the middle ATP-binding domain (residues 103-357) and the C-terminal tripeptide-binding domain (residues 358-505)
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purified enzyme, X-ray diffraction structure determination and analysis at 1.65 A resolution
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recombinant His-tagged enzyme from Escherichia coli strain MLD2502 by nickel and cobalt affinity chromatography
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gene mpl, chromosomal mpl gene, DNA and mino acid sequence determination and analysis, expression of His-tagged enzyme in an Escherichia coli chromosomal mpl gene-deficient mutant strain MLD2502
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Das, D.; Herve, M.; Feuerhelm, J.; Farr, C.; Chiu, H.; Elsliger, M.; Knuth, M.; Klock, H.; Miller, M.; Godzik, A.; Lesley, S.; Deacon, A.; Mengin-Lecreulx, D.; Wilson, I.
Structure and function of the first full-length murein peptide ligase (Mpl) cell wall recycling protein
PLoS ONE
6
e17624
2011
Psychrobacter arcticus (Q4FVQ2), Psychrobacter arcticus 273-4 (Q4FVQ2), Psychrobacter arcticus 273-4
brenda
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