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Information on EC 6.3.2.B2 - murein peptide ligase for references in articles please use BRENDA:EC6.3.2.B2
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EC Tree
The expected taxonomic range for this enzyme is: Psychrobacter arcticus
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Mpl
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-N-acetylmuramate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
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L-alanyl-gamma-D-glutamyl-meso-diaminopimelate:UDP-N-acetylmuramate ligase (ADP-forming)
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ATP + L-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
ATP + L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-L-lysyl-D-alanine
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
Q4FVQ2
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additional information
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ATP + L-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
Q4FVQ2
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ATP + L-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
Q4FVQ2
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additional information
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Q4FVQ2
substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala
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additional information
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substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala
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additional information
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Q4FVQ2
substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
Q4FVQ2
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ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
Q4FVQ2
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0.0117
L-alanine
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.023
L-alanyl-gamma-D-glutamate
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.0042
L-alanyl-gamma-D-glutamyl-L-lysine
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.0017
L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
4.33
L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.098
L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.088
L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
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gene mpl
Q4FVQ2
UniProt
brenda
gene mpl
Q4FVQ2
UniProt
brenda
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Q4FVQ2
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brenda
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brenda
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evolution
Q4FVQ2
Mpl and MurC-F enzymes have three domains that are classified in Pfam families, PF01225 Mur ligase catalytic domain, PF08245 Mur ligase middle domain, and PF02875 Mur ligase glutamate binding/C-terminal domain, structure comparisons, overview
evolution
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Mpl and MurC-F enzymes have three domains that are classified in Pfam families, PF01225 Mur ligase catalytic domain, PF08245 Mur ligase middle domain, and PF02875 Mur ligase glutamate binding/C-terminal domain, structure comparisons, overview
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physiological function
Q4FVQ2
in Gram-negative bacteria, 30-60% of the bacterial cell wall is recycled during each generation. Part of this recycling process involves the murein peptide ligase, which attaches the breakdown product, the tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate, to UDP-MurNAc
physiological function
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in Gram-negative bacteria, 30-60% of the bacterial cell wall is recycled during each generation. Part of this recycling process involves the murein peptide ligase, which attaches the breakdown product, the tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate, to UDP-MurNAc
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additional information
Q4FVQ2
unique sequence-structure relationships of Mpl proteins, overview, Residues Arg357, Arg358, Phe374, Ala375, His376, His377, Glu402, Pro403, Arg404, Ser405, Asn406, Thr407, Ser483, Asn484 and Gly485 may be important in binding tri, tetra, and pentapeptide substrates
additional information
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unique sequence-structure relationships of Mpl proteins, overview, Residues Arg357, Arg358, Phe374, Ala375, His376, His377, Glu402, Pro403, Arg404, Ser405, Asn406, Thr407, Ser483, Asn484 and Gly485 may be important in binding tri, tetra, and pentapeptide substrates
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57000
Q4FVQ2
x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
57187
Q4FVQ2
x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
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Q4FVQ2
x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
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x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
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additional information
Q4FVQ2
PaMpl can be divided into 3 distinct domains: the N-terminal UDPMurNAc-binding domain (residues 1-102), the middle ATP-binding domain (residues 103-357) and the C-terminal tripeptide-binding domain (residues 358-505)
additional information
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PaMpl can be divided into 3 distinct domains: the N-terminal UDPMurNAc-binding domain (residues 1-102), the middle ATP-binding domain (residues 103-357) and the C-terminal tripeptide-binding domain (residues 358-505)
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purified enzyme, X-ray diffraction structure determination and analysis at 1.65 A resolution
Q4FVQ2
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recombinant His-tagged enzyme from Escherichia coli strain MLD2502 by nickel and cobalt affinity chromatography
Q4FVQ2
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gene mpl, chromosomal mpl gene, DNA and mino acid sequence determination and analysis, expression of His-tagged enzyme in an Escherichia coli chromosomal mpl gene-deficient mutant strain MLD2502
Q4FVQ2
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Das, D.; Herve, M.; Feuerhelm, J.; Farr, C.; Chiu, H.; Elsliger, M.; Knuth, M.; Klock, H.; Miller, M.; Godzik, A.; Lesley, S.; Deacon, A.; Mengin-Lecreulx, D.; Wilson, I.
Structure and function of the first full-length murein peptide ligase (Mpl) cell wall recycling protein
PLoS ONE
6
e17624
2011
Psychrobacter arcticus (Q4FVQ2), Psychrobacter arcticus 273-4, Psychrobacter arcticus 273-4 (Q4FVQ2)
brenda
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