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Information on EC 6.3.2.B19 - [LysW]-C-terminal-L-glutamate ligase
for references in articles please use BRENDA:EC6.3.2.B19
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Please check here for current data: EC unknown
Please check here for current data: EC unknown
preliminary BRENDA-supplied EC number
EC Tree 6 Ligases
6.3 Forming carbon-nitrogen bonds
6.3.2 Acid—amino-acid ligases (peptide synthases)
6.3.2.B19 [LysW]-C-terminal-L-glutamate ligase
The expected taxonomic range for this enzyme is: Archaea, Eukaryota
Reaction Schemes
showSynonyms
ArgX, Saci_1621, StArgX, STK_15050, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ArgX
Saci_1621
StArgX
STK_15050
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate = ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
[LysW]-C-terminal-L-glutamate:L-glutamate ligase (ADP-forming)
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
Substrates: arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: -
Products: -
Products: -
?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Substrates: -
Products: -
Products: -
?
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
malfunction
no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible
malfunction
-
no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible
-
malfunction
-
no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible
-
malfunction
-
no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible
-
malfunction
-
no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible
-
malfunction
-
no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible
-
physiological function
arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
physiological function
-
arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
-
physiological function
-
arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
-
physiological function
-
arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
-
physiological function
-
arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
-
physiological function
-
arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ARGX_SULAC
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
282
0
31715
Swiss-Prot
-
ARGX_SULTO
Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
282
0
31534
Swiss-Prot
other Location (Reliability: 5)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
X-ray diffraction with resolution of 1.8 A of the enzyme complexed with LysW, L-glutamate, ADP, Mg2+, Zn2+ and sulfate. X-ray diffraction with resolution of 1.87 A of the enzyme complexed with ADP
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ouchi, T.; Tomita, T.; Horie, A.; Yoshida, A.; Takahashi, K.; Nishida, H.; Lassak, K.; Taka, H.; Mineki, R.; Fujimura, T.; Kosono, S.; Nishiyama, C.; Masui, R.; Kuramitsu, S.; Albers, S.V.; Kuzuyama, T.; Nishiyama, M.
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus
Nat. Chem. Biol.
9
277-283
2013
Sulfolobus acidocaldarius (Q4J8E7), Sulfurisphaera tokodaii (Q970U6), Sulfurisphaera tokodaii 7 (Q970U6), Sulfolobus acidocaldarius DSM 639 (Q4J8E7)
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